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WO2003004621A3 - Activite de sir2 - Google Patents

Activite de sir2 Download PDF

Info

Publication number
WO2003004621A3
WO2003004621A3 PCT/US2002/021461 US0221461W WO03004621A3 WO 2003004621 A3 WO2003004621 A3 WO 2003004621A3 US 0221461 W US0221461 W US 0221461W WO 03004621 A3 WO03004621 A3 WO 03004621A3
Authority
WO
WIPO (PCT)
Prior art keywords
sir2
activity
screening
compounds
compound
Prior art date
Application number
PCT/US2002/021461
Other languages
English (en)
Other versions
WO2003004621A2 (fr
Inventor
Leonard Guarente
Homayoun Vaziri
Shin-Ichiro Imai
Wei Gu
Original Assignee
Elixir Pharmaceuticals Inc
Leonard Guarente
Homayoun Vaziri
Shin-Ichiro Imai
Wei Gu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elixir Pharmaceuticals Inc, Leonard Guarente, Homayoun Vaziri, Shin-Ichiro Imai, Wei Gu filed Critical Elixir Pharmaceuticals Inc
Priority to CA002453195A priority Critical patent/CA2453195A1/fr
Priority to EP02749837A priority patent/EP1529115A4/fr
Priority to AU2002320325A priority patent/AU2002320325B2/en
Publication of WO2003004621A2 publication Critical patent/WO2003004621A2/fr
Publication of WO2003004621A3 publication Critical patent/WO2003004621A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4703—Regulators; Modulating activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne des procédés de criblage de composés modulant des processus cellulaires et organismiques par modification de l'activité de SIR2 et/ou de facteurs de transcription, par exemple, p53 ; notamment, des procédés de criblage de composés modifiant la durée de vie et/ou le métabolisme d'une cellule ou d'un organisme par modulation de l'activité de SIR2 et/ou de facteurs de transcription, par exemple, p53 ; et plus précisément, des procédés de criblage de composés modulant l'activité de Sir2 et/ou de facteurs de transcription, par exemple, p53. Plus précisément, l'invention concerne un procédé de criblage d'un composé consistant à utiliser un mélange d'essai renfermant un facteur de transcription de Sir2 et un cofacteur de Sir2 avec le composé, ainsi qu'à évaluer une activité d'un composant du mélange d'essai en présence du composé. L'invention concerne également des utilisations thérapeutiques desdits composés. L'invention concerne enfin un procédé de modification du statut d'acétylation d'un site de liaison de facteur de transcription sur une histone ou un ADN par élévation des concentrations locales de Sir2.
PCT/US2002/021461 2001-07-06 2002-07-08 Activite de sir2 WO2003004621A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002453195A CA2453195A1 (fr) 2001-07-06 2002-07-08 Activite de sir2
EP02749837A EP1529115A4 (fr) 2001-07-06 2002-07-08 Activite de sir2
AU2002320325A AU2002320325B2 (en) 2001-07-06 2002-07-08 SIR2 activity

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US30337001P 2001-07-06 2001-07-06
US30345601P 2001-07-06 2001-07-06
US60/303,456 2001-07-06
US60/303,370 2001-07-06
US19015902A 2002-07-05 2002-07-05
US10/190,159 2002-07-05

Publications (2)

Publication Number Publication Date
WO2003004621A2 WO2003004621A2 (fr) 2003-01-16
WO2003004621A3 true WO2003004621A3 (fr) 2005-03-17

Family

ID=32074257

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/021461 WO2003004621A2 (fr) 2001-07-06 2002-07-08 Activite de sir2

Country Status (4)

Country Link
EP (1) EP1529115A4 (fr)
AU (1) AU2002320325B2 (fr)
CA (1) CA2453195A1 (fr)
WO (1) WO2003004621A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8642284B1 (en) 1999-12-15 2014-02-04 Massachusetts Institute Of Technology Methods for identifying agents that alter NAD-dependent deacetylation activity of a SIR2 protein
US7452664B2 (en) 1999-12-15 2008-11-18 Massachusetts Institute Of Technology Methods for identifying agents which alter histone protein acetylation
US7572575B2 (en) 2000-12-13 2009-08-11 Massachusetts Institute Of Technology SIR2 activity
US7514406B2 (en) 2001-11-27 2009-04-07 Fred Hutchinson Cancer Research Center Methods for inhibiting deacetylase activity
US7351542B2 (en) * 2002-05-20 2008-04-01 The Regents Of The University Of California Methods of modulating tubulin deacetylase activity
AU2003237248A1 (en) 2002-05-23 2003-12-12 The Regents Of The University Of California Methods of modulating mitochondrial nad-dependent deacetylase
US20070099830A1 (en) 2005-04-21 2007-05-03 Massachusetts Institute Of Technology Sirt4 activities
US8518635B2 (en) 2006-06-12 2013-08-27 The J. David Gladstone Institutes Regulation of protein activity by reversible acetylation

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LANDRY ET AL.: "The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases", PNAS, vol. 97, no. 11, 23 May 2000 (2000-05-23), pages 5807 - 5811, XP002963941 *
MARKS ET AL.: "Histone deacetylase inhibitors: inducers of differentiation or apoptosis of transformed cells", JOURNAL OF THE NATIONAL CANCER INSTITUTE, vol. 92, no. 15, 2 August 2000 (2000-08-02), pages 1210 - 1216, XP001121734 *
See also references of EP1529115A4 *

Also Published As

Publication number Publication date
AU2002320325A2 (en) 2003-01-21
AU2002320325B2 (en) 2008-04-03
EP1529115A4 (fr) 2005-08-24
WO2003004621A2 (fr) 2003-01-16
EP1529115A2 (fr) 2005-05-11
CA2453195A1 (fr) 2003-01-16

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