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WO2003002766A3 - Method for detection of foot-and-mouth disease virus - Google Patents

Method for detection of foot-and-mouth disease virus Download PDF

Info

Publication number
WO2003002766A3
WO2003002766A3 PCT/GB2002/002943 GB0202943W WO03002766A3 WO 2003002766 A3 WO2003002766 A3 WO 2003002766A3 GB 0202943 W GB0202943 W GB 0202943W WO 03002766 A3 WO03002766 A3 WO 03002766A3
Authority
WO
WIPO (PCT)
Prior art keywords
seq
polynucleotide
sequence
fragment
sample
Prior art date
Application number
PCT/GB2002/002943
Other languages
French (fr)
Other versions
WO2003002766A2 (en
Inventor
Scott Maclean Reid
Soren Alexandersen
Original Assignee
Animal Health Inst
Scott Maclean Reid
Soren Alexandersen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Animal Health Inst, Scott Maclean Reid, Soren Alexandersen filed Critical Animal Health Inst
Priority to AU2002311477A priority Critical patent/AU2002311477A1/en
Publication of WO2003002766A2 publication Critical patent/WO2003002766A2/en
Publication of WO2003002766A3 publication Critical patent/WO2003002766A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides a polynucleotide having the sequence of SEQ ID NO:1 (CCTCGGGGTACCTGAAGGGCATCC) or a fragment thereof, which fragment is capable of binding specifically to the complementary sequence of SEQ ID NO: 1, a polynucleotide having the sequence of SEQ ID NO:2 (CAC{T/C}T{T/C}AAG{G/A}TGACA{T/C}TG{G/A}TACTG GTAC) and a polynucleotide having the sequence of SEQ ID NO:3 (CAGAT{C/T}CC{G/A}AGTG{T/A}C{I}C{I}TGTTA). The invention also provides a method for determining whether a sample contains FMDV comprising (a) contacting the sample with a probe comprising a polynucleotide having the sequence of SEQ ID NO:1 (CCTCGGGGTACCTGAAGGGCATCC) or a fragment thereof, which fragment is capable of binding specifically to the complementary sequence of SEQ ID NO: 1, in vitro under conditions that allow the probe to bind specifically to a target polynucleotide; and (b) determining whether the probe has bound to a target polynucleotide; thereby to determine whether the sample comprises a target polynucleotide, the presence of a target polynucleotide in a sample being indicative of presence of FMDV.
PCT/GB2002/002943 2001-06-28 2002-06-27 Method for detection of foot-and-mouth disease virus WO2003002766A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002311477A AU2002311477A1 (en) 2001-06-28 2002-06-27 Method for detection of foot-and-mouth disease virus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0115777A GB2377017A (en) 2001-06-28 2001-06-28 Detection of foot and mouth disease virus
GB0115777.5 2001-06-28

Publications (2)

Publication Number Publication Date
WO2003002766A2 WO2003002766A2 (en) 2003-01-09
WO2003002766A3 true WO2003002766A3 (en) 2003-08-28

Family

ID=9917500

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/002943 WO2003002766A2 (en) 2001-06-28 2002-06-27 Method for detection of foot-and-mouth disease virus

Country Status (3)

Country Link
AU (1) AU2002311477A1 (en)
GB (1) GB2377017A (en)
WO (1) WO2003002766A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE474933T1 (en) * 2002-12-06 2010-08-15 Hoffmann La Roche METHOD FOR DETECTING PATHOGENIC ORGANISMS
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
CN100381170C (en) * 2003-09-03 2008-04-16 上海华谊生物技术有限公司 Foot and mouth disease bivalent polypeptide vaccine and its preparation method and use
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20120122099A1 (en) 2003-09-11 2012-05-17 Rangarajan Sampath Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20070042388A1 (en) * 2005-08-12 2007-02-22 Wong Christopher W Method of probe design and/or of nucleic acids detection
US20070065814A1 (en) * 2005-09-21 2007-03-22 Engelhard Eric K Detecting foot-and-mouth disease virus
WO2009131728A2 (en) * 2008-01-29 2009-10-29 Ibis Biosciences, Inc. Compositions for use in identification of picornaviruses
CN107130014A (en) * 2016-02-29 2017-09-05 天津市农业质量标准与检测技术研究所 Method for rapidly detecting sheep-derived component content in meat product by using internal reference gene

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19732086A1 (en) * 1997-07-25 1999-01-28 Univ Leipzig Quantification of eubacteria in competitive polymerase chain reaction
WO2000011016A1 (en) * 1998-08-25 2000-03-02 The General Hospital Corporation Dysferlin mutations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19732086A1 (en) * 1997-07-25 1999-01-28 Univ Leipzig Quantification of eubacteria in competitive polymerase chain reaction
WO2000011016A1 (en) * 1998-08-25 2000-03-02 The General Hospital Corporation Dysferlin mutations

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ALEXANDERSEN S ET AL: "Development of reverse transcription-PCR (oligonucleotide probing) enzyme-linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease: a new system using simple and aqueous-phase hybridization.", JOURNAL OF CLINICAL MICROBIOLOGY. UNITED STATES DEC 2000, vol. 38, no. 12, December 2000 (2000-12-01), pages 4604 - 4613, XP002244065, ISSN: 0095-1137 *
DATABASE EMBL [online] 7 November 1985 (1985-11-07), XP002244069, retrieved from EBI Database accession no. X00871 *
REID S M ET AL: "Diagnosis of foot-and-mouth disease by real-time fluorogenic PCR assay.", THE VETERINARY RECORD. ENGLAND 17 NOV 2001, vol. 149, no. 20, 17 November 2001 (2001-11-17), pages 621 - 623, XP009012159, ISSN: 0042-4900 *
REID S M ET AL: "Diagnosis of foot-and-mouth disease by RT-PCR: use of phylogenetic data to evaluate primers for the typing of viral RNA in clinical samples.", ARCHIVES OF VIROLOGY. AUSTRIA DEC 2001, vol. 146, no. 12, December 2001 (2001-12-01), pages 2421 - 2434, XP002244067, ISSN: 0304-8608 *
REID S M ET AL: "Primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction.", JOURNAL OF VIROLOGICAL METHODS. NETHERLANDS SEP 2000, vol. 89, no. 1-2, September 2000 (2000-09-01), pages 167 - 176, XP002244066, ISSN: 0166-0934 *
REID SCOTT M ET AL: "Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay.", JOURNAL OF VIROLOGICAL METHODS. NETHERLANDS AUG 2002, vol. 105, no. 1, August 2002 (2002-08-01), pages 67 - 80, XP002244068, ISSN: 0166-0934 *

Also Published As

Publication number Publication date
GB0115777D0 (en) 2001-08-22
WO2003002766A2 (en) 2003-01-09
AU2002311477A1 (en) 2003-03-03
GB2377017A (en) 2002-12-31

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