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WO2003002759A3 - Nachweis spezifischer dinukleotide in dna-proben durch fluoreszenzresonanztransfer (fret) - Google Patents

Nachweis spezifischer dinukleotide in dna-proben durch fluoreszenzresonanztransfer (fret) Download PDF

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Publication number
WO2003002759A3
WO2003002759A3 PCT/DE2002/002432 DE0202432W WO03002759A3 WO 2003002759 A3 WO2003002759 A3 WO 2003002759A3 DE 0202432 W DE0202432 W DE 0202432W WO 03002759 A3 WO03002759 A3 WO 03002759A3
Authority
WO
WIPO (PCT)
Prior art keywords
fret
dna
energy transfer
resonance energy
fluorescence resonance
Prior art date
Application number
PCT/DE2002/002432
Other languages
English (en)
French (fr)
Other versions
WO2003002759A2 (de
Inventor
David Guetig
Original Assignee
Epigenomics Ag
David Guetig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag, David Guetig filed Critical Epigenomics Ag
Priority to US10/482,339 priority Critical patent/US20040248120A1/en
Priority to EP02747242A priority patent/EP1412524A2/de
Publication of WO2003002759A2 publication Critical patent/WO2003002759A2/de
Publication of WO2003002759A3 publication Critical patent/WO2003002759A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Es wird ein Verfahren zur Detektion spezifischer Dinukleotide in einer DNA-Probe beschrieben. Eine Polymerase-Kettenreaktion (PCR) wird unter Verwendung a) eines Nukleotids, das Teil des zu detektierenden Dinukleotids ist, von dem eine ausreichende Menge durch ein Donor-Fluorphor markiert und b) ein anderes Nukleotid, das Teil des zu detektierenden Dinukleotids ist, von dem eine ausreichende Menge mit einem Akzeptor-Fluorophor markiert ist, durchgeführt. Das Verfahren bestimmt oder quantifiziert die Anwesenheit des Dinukleotids durch Messung des Ausmaßes des Fluoreszenzresonanzenergietransfers (FRET) zwischen dem Donor- und Akzeptor-Fluorophor.
PCT/DE2002/002432 2001-06-27 2002-06-27 Nachweis spezifischer dinukleotide in dna-proben durch fluoreszenzresonanztransfer (fret) WO2003002759A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/482,339 US20040248120A1 (en) 2001-06-27 2002-06-27 Detection of specific dinucleotides in dna-samples by fluorescence resonance energy transfer (fret)
EP02747242A EP1412524A2 (de) 2001-06-27 2002-06-27 Nachweis spezifischer dinukleotide in dna-proben durch fluoreszenzresonanztransfer (fret)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10132211.9 2001-06-27
DE10132211A DE10132211A1 (de) 2001-06-27 2001-06-27 Nachweis spezifischer Dinukleotide in DNA-Proben durch Fluoreszenzresonanzenergietransfer (FRET)

Publications (2)

Publication Number Publication Date
WO2003002759A2 WO2003002759A2 (de) 2003-01-09
WO2003002759A3 true WO2003002759A3 (de) 2003-05-30

Family

ID=7690450

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/002432 WO2003002759A2 (de) 2001-06-27 2002-06-27 Nachweis spezifischer dinukleotide in dna-proben durch fluoreszenzresonanztransfer (fret)

Country Status (4)

Country Link
US (1) US20040248120A1 (de)
EP (1) EP1412524A2 (de)
DE (1) DE10132211A1 (de)
WO (1) WO2003002759A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10347400B4 (de) * 2003-10-09 2005-08-04 Epigenomics Ag Verbesserte Bisulfit-Umwandlung von DNA durch kurzzeitige Temperaturerhöhungen
US20060134643A1 (en) * 2000-06-19 2006-06-22 Kurt Berlin Bisulfite conversion of DNA
DE10347396B4 (de) * 2003-10-09 2005-06-23 Epigenomics Ag Optimierte Bisulfit-Umwandlung von DNA durch Einsatz von Dioxan
DE10347399B4 (de) * 2003-10-09 2005-09-15 Epigenomics Ag Bisulfit-Umwandlung zum Nachweis von Cytosin-Methylierungen in DNA mittels optimierter Aufreinigung
DE10347397B4 (de) * 2003-10-09 2005-08-04 Epigenomics Ag Optimierte Bisulfit-Umwandlung durch Zusatz von n-Alkylenglykol-Verbindungen
DE10304219B3 (de) * 2003-01-30 2004-08-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
CA3137726A1 (en) * 2019-04-22 2020-10-29 Behavioral Diagnostics, Llc Compositions and methods for correcting for cellular admixture in epigenetic analyses

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229943A2 (de) * 1985-12-23 1987-07-29 Molecular Biosystems, Inc. Fluoreszierende stokessche Shift-Sonden für Polynucleotidhybridisationsproben
WO1997022719A1 (en) * 1995-12-18 1997-06-26 Washington University Method for nucleic acid analysis using fluorescence resonance energy transfer
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
WO2000070090A1 (en) * 1999-05-14 2000-11-23 University Of Southern California Process for high throughput dna methylation analysis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3807975C2 (de) * 1988-03-10 2002-03-07 Karl Otto Greulich Verfahren zur optischen Charakterisierung von Nukleinsäuren und Oligonukleotiden
JP4540754B2 (ja) * 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
CN1251609A (zh) * 1997-02-12 2000-04-26 尤金·Y·查恩 分析聚合物的方法和产品
US6642001B1 (en) * 1999-07-13 2003-11-04 Whitehead Institute For Biomedical Research Generic SBE-FRET protocol
DE19935772C2 (de) * 1999-07-26 2002-11-07 Epigenomics Ag Verfahren zur relativen Quantifizierung der Methylierung von Cytosin Basen in DNA-Proben

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229943A2 (de) * 1985-12-23 1987-07-29 Molecular Biosystems, Inc. Fluoreszierende stokessche Shift-Sonden für Polynucleotidhybridisationsproben
WO1997022719A1 (en) * 1995-12-18 1997-06-26 Washington University Method for nucleic acid analysis using fluorescence resonance energy transfer
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
WO2000070090A1 (en) * 1999-05-14 2000-11-23 University Of Southern California Process for high throughput dna methylation analysis

Also Published As

Publication number Publication date
US20040248120A1 (en) 2004-12-09
WO2003002759A2 (de) 2003-01-09
DE10132211A1 (de) 2003-01-16
EP1412524A2 (de) 2004-04-28

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