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WO2003093795A3 - Device and method for analytical cell imaging - Google Patents

Device and method for analytical cell imaging Download PDF

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Publication number
WO2003093795A3
WO2003093795A3 PCT/US2003/013842 US0313842W WO03093795A3 WO 2003093795 A3 WO2003093795 A3 WO 2003093795A3 US 0313842 W US0313842 W US 0313842W WO 03093795 A3 WO03093795 A3 WO 03093795A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
immunomagnetically
chamber
enriched
tumor
Prior art date
Application number
PCT/US2003/013842
Other languages
French (fr)
Other versions
WO2003093795A2 (en
Inventor
Michael Trainer
Original Assignee
Immunivest Corp
Michael Trainer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunivest Corp, Michael Trainer filed Critical Immunivest Corp
Priority to JP2004501911A priority Critical patent/JP2005524833A/en
Priority to CA002484730A priority patent/CA2484730A1/en
Priority to EP03728685A priority patent/EP1501917A4/en
Priority to AU2003234459A priority patent/AU2003234459A1/en
Publication of WO2003093795A2 publication Critical patent/WO2003093795A2/en
Publication of WO2003093795A3 publication Critical patent/WO2003093795A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Signal Processing (AREA)
  • Optics & Photonics (AREA)
  • Hospice & Palliative Care (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

In patients having carcinomas tumor cells shed into the blood, enumeration and characterization of these cells offers the opportunity to obtain a 'real time' biopsy of the tumor and may improve the management of the disease. The frequency of circulating tumor cells is rare (<1cell/ml) and technology is needed that has sufficient sensitivity and specificity to enumerate and characterize these cells. The present system was developed to provide an immunophenotype, based on fluorescence waveforms as well as images of immunomagnetically-enriched cells. Blood volumes ranging from 7.5-30ml are immunomagnetically enriched for epithelial cells. The sample volume is reduced to 320ul and inserted into an analysis chamber. Upon introduction of the chamber in a magnetic field, the immunomagnetically tagged cells rise out of the sample and align between nickel lines (period 30µm, space 15µm) that are present on the viewing surface of the chamber.
PCT/US2003/013842 2002-05-03 2003-05-05 Device and method for analytical cell imaging WO2003093795A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2004501911A JP2005524833A (en) 2002-05-03 2003-05-05 Devices and methods for analytical cell imaging
CA002484730A CA2484730A1 (en) 2002-05-03 2003-05-05 Device and method for analytical cell imaging
EP03728685A EP1501917A4 (en) 2002-05-03 2003-05-05 Device and method for analytical cell imaging
AU2003234459A AU2003234459A1 (en) 2002-05-03 2003-05-05 Device and method for analytical cell imaging

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37786802P 2002-05-03 2002-05-03
US60/377,868 2002-05-03

Publications (2)

Publication Number Publication Date
WO2003093795A2 WO2003093795A2 (en) 2003-11-13
WO2003093795A3 true WO2003093795A3 (en) 2004-10-21

Family

ID=29401578

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/013842 WO2003093795A2 (en) 2002-05-03 2003-05-05 Device and method for analytical cell imaging

Country Status (5)

Country Link
EP (1) EP1501917A4 (en)
JP (1) JP2005524833A (en)
AU (1) AU2003234459A1 (en)
CA (1) CA2484730A1 (en)
WO (1) WO2003093795A2 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8986966B2 (en) 2002-09-27 2015-03-24 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US9174222B2 (en) 2005-04-05 2015-11-03 The General Hospital Corporation Devices and method for enrichment and alteration of cells and other particles

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4041458B2 (en) * 2003-12-12 2008-01-30 日立ソフトウエアエンジニアリング株式会社 Fluorescent bead detection method and fluorescent bead detection device
CA2576510C (en) 2004-08-06 2014-10-14 Compucyte Corporation Multiple-color monochromatic light absorption and quantification of light absorption in a stained sample
EP1810021A1 (en) * 2004-10-22 2007-07-25 Fermiscan Australia Pty Limited Analytical method and apparatus
US9134237B2 (en) 2005-09-20 2015-09-15 Janssen Diagnotics, LLC High sensitivity multiparameter method for rare event analysis in a biological sample
EP3211086B1 (en) 2005-09-20 2021-06-09 Menarini Silicon Biosystems S.p.A. Methods and composition to generate unique sequence dna probes, labeling of dna probes and the use of these probes
FI20051329A0 (en) 2005-12-27 2005-12-27 Wallac Oy Apparatus and method for optical measurement of samples
CA2652027C (en) * 2006-05-12 2017-06-06 Immunivest Corporation A laser illumination system in fluorescent microscopy
EP2029779A4 (en) 2006-06-14 2010-01-20 Living Microsystems Inc Use of highly parallel snp genotyping for fetal diagnosis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
WO2008012724A2 (en) * 2006-07-20 2008-01-31 Koninklijke Philips Electronics N.V. Optical tracking and position determination for detection methods and systems
US7782452B2 (en) * 2007-08-31 2010-08-24 Kla-Tencor Technologies Corp. Systems and method for simultaneously inspecting a specimen with two distinct channels
US8143600B2 (en) * 2008-02-18 2012-03-27 Visiongate, Inc. 3D imaging of live cells with ultraviolet radiation
JP5119114B2 (en) * 2008-09-30 2013-01-16 富士フイルム株式会社 Light source device and photographing device
JP4805418B1 (en) * 2010-03-31 2011-11-02 古河電気工業株式会社 Cell identification device and identification method
KR20150023022A (en) 2011-03-24 2015-03-04 앤팩 바이오-메디컬 사이언스 시오., 엘티디. Micro devices for disease detection
CN106929401B (en) * 2011-05-05 2020-06-09 安派科生物医学科技有限公司 Tumor cell detector
WO2013006828A1 (en) * 2011-07-07 2013-01-10 Scripps Health Method of analyzing cardiovascular disorders and uses thereof
US9511152B2 (en) * 2012-04-05 2016-12-06 The Board Of Regents Of The University Of Texas System Multicolored pH-activatable fluorescence nanoplatform
CN104914078A (en) * 2015-04-16 2015-09-16 南京昊控软件技术有限公司 Large-range multi-point fluid concentration measuring system
KR102392976B1 (en) * 2016-03-07 2022-04-29 엑스-젤 인코포레이티드 Compositions and methods for the identification of rare cells
FR3051556A1 (en) * 2016-05-18 2017-11-24 Biocytex USE OF FLUORESCENT MARKERS FOR THE DETECTION OF BIOLOGICAL TARGET IN A SAMPLE

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034613A (en) * 1989-11-14 1991-07-23 Cornell Research Foundation, Inc. Two-photon laser microscopy
US5127730A (en) * 1990-08-10 1992-07-07 Regents Of The University Of Minnesota Multi-color laser scanning confocal imaging system
US5479252A (en) * 1993-06-17 1995-12-26 Ultrapointe Corporation Laser imaging system for inspection and analysis of sub-micron particles
US5691839A (en) * 1993-04-15 1997-11-25 Kowa Company Ltd. Laser scanning optical microscope
US5698397A (en) * 1995-06-07 1997-12-16 Sri International Up-converting reporters for biological and other assays using laser excitation techniques

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034613A (en) * 1989-11-14 1991-07-23 Cornell Research Foundation, Inc. Two-photon laser microscopy
US5127730A (en) * 1990-08-10 1992-07-07 Regents Of The University Of Minnesota Multi-color laser scanning confocal imaging system
US5691839A (en) * 1993-04-15 1997-11-25 Kowa Company Ltd. Laser scanning optical microscope
US5479252A (en) * 1993-06-17 1995-12-26 Ultrapointe Corporation Laser imaging system for inspection and analysis of sub-micron particles
US5698397A (en) * 1995-06-07 1997-12-16 Sri International Up-converting reporters for biological and other assays using laser excitation techniques

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8986966B2 (en) 2002-09-27 2015-03-24 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US9174222B2 (en) 2005-04-05 2015-11-03 The General Hospital Corporation Devices and method for enrichment and alteration of cells and other particles
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US9273355B2 (en) 2006-06-14 2016-03-01 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags
US8296076B2 (en) 2008-09-20 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuoploidy by sequencing
US8682594B2 (en) 2008-09-20 2014-03-25 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9353414B2 (en) 2008-09-20 2016-05-31 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9404157B2 (en) 2008-09-20 2016-08-02 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing

Also Published As

Publication number Publication date
WO2003093795A2 (en) 2003-11-13
AU2003234459A8 (en) 2003-11-17
EP1501917A2 (en) 2005-02-02
CA2484730A1 (en) 2003-11-13
AU2003234459A1 (en) 2003-11-17
EP1501917A4 (en) 2006-04-19
JP2005524833A (en) 2005-08-18

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