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WO2003068978A3 - Combinaisons de vecteurs et leurs hotes pour propager et exprimer des genes recombinants sans recourir a des antibiotiques - Google Patents

Combinaisons de vecteurs et leurs hotes pour propager et exprimer des genes recombinants sans recourir a des antibiotiques Download PDF

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Publication number
WO2003068978A3
WO2003068978A3 PCT/FR2003/000481 FR0300481W WO03068978A3 WO 2003068978 A3 WO2003068978 A3 WO 2003068978A3 FR 0300481 W FR0300481 W FR 0300481W WO 03068978 A3 WO03068978 A3 WO 03068978A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression
recourse
hosts
antibiotics
vectors
Prior art date
Application number
PCT/FR2003/000481
Other languages
English (en)
Other versions
WO2003068978A2 (fr
Inventor
Philippe Marliere
Volker Doring
Original Assignee
Evologic Sa
Philippe Marliere
Volker Doring
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evologic Sa, Philippe Marliere, Volker Doring filed Critical Evologic Sa
Priority to AU2003226483A priority Critical patent/AU2003226483A1/en
Publication of WO2003068978A2 publication Critical patent/WO2003068978A2/fr
Publication of WO2003068978A3 publication Critical patent/WO2003068978A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention se rapporte à de nouveaux systèmes eubactériens d'expression de protéines, utilisant le gène dapA comme marqueur de sélection, ainsi qu'à leur utilisation pour la production de protéines recombinantes.
PCT/FR2003/000481 2002-02-14 2003-02-14 Combinaisons de vecteurs et leurs hotes pour propager et exprimer des genes recombinants sans recourir a des antibiotiques WO2003068978A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003226483A AU2003226483A1 (en) 2002-02-14 2003-02-14 Combinations of vectors and the hosts thereof for the propagation and expression of recombinant genes without recourse to antibiotics

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0201835A FR2835848A1 (fr) 2002-02-14 2002-02-14 Combinaisons de vecteurs et leurs hotes pour propager et exprimer des genes recombinants sans recourir a des antibiotiques
FR02/01835 2002-02-14

Publications (2)

Publication Number Publication Date
WO2003068978A2 WO2003068978A2 (fr) 2003-08-21
WO2003068978A3 true WO2003068978A3 (fr) 2004-03-25

Family

ID=27620207

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2003/000481 WO2003068978A2 (fr) 2002-02-14 2003-02-14 Combinaisons de vecteurs et leurs hotes pour propager et exprimer des genes recombinants sans recourir a des antibiotiques

Country Status (3)

Country Link
AU (1) AU2003226483A1 (fr)
FR (1) FR2835848A1 (fr)
WO (1) WO2003068978A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201021795D0 (en) * 2010-12-21 2011-02-02 Msd Biolog Uk Ltd Expression Process

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0435132A1 (fr) * 1989-12-27 1991-07-03 Forschungszentrum Jülich Gmbh Procédé de la production fermentative d'aminoacides, en particulier de L-lysine, micro-organismes propres à cela et ADN recombinant
WO2002059292A2 (fr) * 2000-10-11 2002-08-01 Washington University Systemes d'hotes-vecteurs a stabilisation letale fonctionnelle

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0435132A1 (fr) * 1989-12-27 1991-07-03 Forschungszentrum Jülich Gmbh Procédé de la production fermentative d'aminoacides, en particulier de L-lysine, micro-organismes propres à cela et ADN recombinant
WO2002059292A2 (fr) * 2000-10-11 2002-08-01 Washington University Systemes d'hotes-vecteurs a stabilisation letale fonctionnelle

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CIRILLO J D ET AL: "GENETIC DETERMINATION OF THE MESO-DIAMINOPIMELATE BIOSYNTHETIC PATHWAY OF MYCOBACTERIA", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 176, no. 14, July 1994 (1994-07-01), pages 4424 - 4429, XP002907358, ISSN: 0021-9193 *
NAKAYAMA K ET AL: "CONSTRUCTION OF AN ASD+ EXPRESSION-CLONING VECTOR: STABLE MAINTAINANCE AND HIGH LEVEL EXPRESSION OF CLONED GENES IN A SALMONELLA VACCINE STRAIN", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 6, no. 6, 1 June 1988 (1988-06-01), pages 693 - 697, XP000578379, ISSN: 0733-222X *

Also Published As

Publication number Publication date
WO2003068978A2 (fr) 2003-08-21
AU2003226483A1 (en) 2003-09-04
FR2835848A1 (fr) 2003-08-15
AU2003226483A8 (en) 2003-09-04

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