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WO2003054208A2 - Procede permettant de moduler la liberation de saccharides et utilisations dudit procede - Google Patents

Procede permettant de moduler la liberation de saccharides et utilisations dudit procede Download PDF

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Publication number
WO2003054208A2
WO2003054208A2 PCT/CA2002/001917 CA0201917W WO03054208A2 WO 2003054208 A2 WO2003054208 A2 WO 2003054208A2 CA 0201917 W CA0201917 W CA 0201917W WO 03054208 A2 WO03054208 A2 WO 03054208A2
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Prior art keywords
effect
glucosamine
saccharides
monosaccharides
oligomers
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PCT/CA2002/001917
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English (en)
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WO2003054208A3 (fr
Inventor
Isabelle Boucher
Serge Brunet
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Ism Biopolymer Inc.
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Publication date
Application filed by Ism Biopolymer Inc. filed Critical Ism Biopolymer Inc.
Priority to US10/498,002 priority Critical patent/US20050070500A1/en
Priority to EP02787250A priority patent/EP1455835A2/fr
Priority to AU2002351576A priority patent/AU2002351576A1/en
Priority to CA002507870A priority patent/CA2507870A1/fr
Publication of WO2003054208A2 publication Critical patent/WO2003054208A2/fr
Publication of WO2003054208A3 publication Critical patent/WO2003054208A3/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/737—Sulfated polysaccharides, e.g. chondroitin sulfate, dermatan sulfate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
    • A61K31/728—Hyaluronic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A61K47/555—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound pre-targeting systems involving an organic compound, other than a peptide, protein or antibody, for targeting specific cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders

Definitions

  • the invention relates to a method and composition for delayed delivery of chondroprotective, chondrosynthesis stimulating agents.
  • glycosaminoglycans GAG
  • proteoglycans PG
  • GAG glycosaminoglycans
  • PG proteoglycans
  • NAG chondrodegenerative diseases
  • GS and in a lesser manner NAG have been found to offer substantial physical and emotional benefit against the ailments caused by the degradation of joint cartilage and mucosal tissues. It has been speculated that the onset of chondrodegenerative diseases may be caused by one or many factors involving environmental, nutritional, normal aging, genetic and stress factors.
  • chondroprotective agents so as to supply the necessary building blocks for cartilage and mucosal membrane regeneration in an exogenous way or to viscosupplement the joints.
  • Such agents are hyaluronic acid (HY), collagen (CO), chondroitin (CH), alone or in their salt forms.
  • French Patent No. 2,473,887 discloses the use of biochemical precursors of glucosamineglycans for the treatment of vascular disorders of functional or organic origin in which there is insufficient blood flow to the limbs, for asphyxic hypoxydotic symptoms, and in cosmetology, for skin defects caused by insufficient circulation to the skin.
  • the precursors which include N-acetylglucosamine, increase the elasticity of perivascular tissue, resulting in an increase in arterio-capillary blood flow, without having a vasodilatating action.
  • U.S. Patent No. 4,006,224 discloses the treatment of ulcerative colitis or regional enteritis in a mammal by administering monomers of D- glucosamine, or one of its salts. Equal or superior results to the conventional treatments of the two conditions are obtained.
  • the dose is 20-300 mg/kg of
  • D-glucosamine, HC1 daily.
  • a patient with Crohn's Disease that was not affected by ACTH or PrednisoneTM was given D-glucosamine- HC1 subcutaneously. The symptoms stopped after several weeks of treatment.
  • N-Acetylglucosamine and glucosamine units were considered for use in the prevention and treatment of degenerative joint diseases and cartilage loss, and were found to increase the glycosaminoglycans present in the cartilage to restore cartilage.
  • glucosamine may also be considered in connection with arthritis and oral and injected glucosamine may be partially useful for arthrosic patients.
  • chitin and chitosan are important potential sources of NAG and GS.
  • Chitin a polysaccharide, is a bi ⁇ polymer that occurs in nature in greater quantities than any other biopolymer except cellulose. Its most common occurrence is in the shells of crustaceans, e.g.
  • chitin is a polymer of the N-acetyl glucosamine monomer unit, although some of the monomer units in its structure are devoid of the acetyl substituent and deacetylated chitin could more properly be said to be a copolymer of N-acetyl glucosamine and glucosamine monomers, with the latter constituting a variable proportion, generally from 1 to 20 percent of the monomer units, usually substantially 5 to 10 percent.
  • Chitosan is derived from chitin by removal of most of the acetyl substituents on the copolymer, usually by hydrolysis, to leave a copolymer having generally from 5 to 20 percent of N-acetyl glucosamine monomer units and correspondingly from 80 to 95 percent of glucosamine units in its structure. Even if administration, mostly oral, of chitin, chitosan or monomers of their units, namely NAG and GS, has been performed, no application allowed yet an equilibrated exchange between the rate of the body to catalyze its reservoir of important biological molecules and the modulation of its capability to use it for different needs.
  • One object of the present invention is to provide a method of modulating release of monosaccharides in an animal which comprises the steps of: treating a source of polysaccharides to produce oligomers of saccharides of desired length; and administrating at least one of the oligomers as obtained from step a) or physiologically acceptable chemically, biochemically or biologically modified form of the oligomers to an animal in an amount sufficient to allow a modulated lasting release of the monosaccharides and obtain a physiological effect, wherein the lasting release lasts for a period of time proportional to the length of the oligomers, wherein more long is the oligomers, more long is the release of monosaccharides to be assimilated by the animal.
  • the polysaccharide source may be selected from the group of chitin, chitosan, hyaluronan, chondroitin, dermatan, keratan, and derivatives thereof.
  • the monosaccharide can be selected from the group consisting of a monomer of glucosamine, an N-acetylglucosamine (NAG), a galactosamine, an N-acetyl-galactosamine, a galactose, a glucuronate, and an iduronate, or salts derivative thereof.
  • oligomers of saccharides that can be qualified as being based prodrug, can be a tandem of at least one of glucosamine or N- acetylglucosamine.
  • the treatment for preparing the oligomers of saccharides is performed with at least one of enzymatic, biological, chemical, mechanical, or radioactive treatment.
  • the desired length of the oligomers of saccharides consists of between about 2 to 100 saccharides.
  • An oral administration, an intravenous administration, an intramuscular administration, an intraarticular administration, an intrasynovial administration, or a cutaneous application may perform the administrating of the oligomers of saccharides.
  • the physiological effect of glucosamine prodrugs is at least one of chondroregenerative or chondroprotective effect, prebiotic effect, probiotic effect, food additive effect, nutraceutical effect, wounding effect, immunomodulatory effect, systemic anti-inflammatory effect, bacteriostatic effect, anti-fungic effect, hypolipidemic effect, hypoglycemic effect, hypocholesterolemic effect, or anti-oxidant effect.
  • Another object of the present invention is to provide the use of an oligomer of saccharides in the manufacture of a medicament for the treatment of inflammation, or osteoarthritis.
  • composition comprising an oligomer of saccharides having physiological effect selected from the group consisting of a chondroregenerative or a chondroprotective effect, a prebiotic effect, a probiotic effect, a food additive effect, a nutraceutical effect, a wounding effect, a immunomodulatory effect, an systemic anti-inflammatory effect, a bacteriostatic effect, an anti-fungic effect, a hypolipidemic effect, a hypoglycemic effect, a hypocholesterolemic effect, or an anti-oxidant effect, in association with a pharmaceutical or nutraceutical carrier.
  • physiological effect selected from the group consisting of a chondroregenerative or a chondroprotective effect, a prebiotic effect, a probiotic effect, a food additive effect, a nutraceutical effect, a wounding effect, a immunomodulatory effect, an systemic anti-inflammatory effect, a bacteriostatic effect, an anti-fungic effect, a hypolipidemic effect, a hypoglycemic
  • the composition generally comprises a monosaccharide that may be selected from the group consisting of a monomer of a glucosamine, an N- acetylglucosamine (NAG), a galactosamine, an N-acetyl-galactosamine, a galactose, a glucuronate, and an iduronate, or salts derivative thereof.
  • a monosaccharide that may be selected from the group consisting of a monomer of a glucosamine, an N- acetylglucosamine (NAG), a galactosamine, an N-acetyl-galactosamine, a galactose, a glucuronate, and an iduronate, or salts derivative thereof.
  • Another object of the present invention is to provide a prodrug for modulating the in vivo release of a monosaccharide consisting of an oligomer of saccharides units consisting of between about 2 to 100 monosaccharides.
  • Fig. 1 illustrates mean ( ⁇ standard deviation) serum glucosamine levels after oral capsule administration of 21.4 mg/kg nominal dose of glucosamine, Product X (composed of 2 up to 9 saccharides), and Product Y(composed of 2 up to 12 saccharides) in the acute post-dose period (0-2 hours) ;
  • Fig. 2 illustrates mean ( ⁇ standard deviation) serum glucosamine levels after oral capsule administration of 214 mg/kg nominal dose of Product X, and Product Y in the acute post-dose period (0-2 hours);
  • Fig. 3 illustrates mean (+ standard deviation) serum glucosamine levels after intravenous administration of 21.4 mg/kg nominal dose of Product X, and Product Y in the acute post-dose period (0-2 hours);
  • an oral administration of the GAG components as for example, but not limited to, glucosamine, N-acetyl-glucosamine, galactosamine, N-acetyl-galactosamine, glucuronate, iduronate, galactose, alone or mixed, extracted from polysaccharides or biopolymers, such as chitin, chitosan, chondroitin, dermatan, keratan, hyaluronan, in their oligomer form, is advantageous for allowing a lasting release of monosaccharides and stimulating the growth and repairing connective tissue and articular cartilage.
  • the oligosaccharide format of the said components is easily absorbed through the gastrointestinal tract and uptaken through the circulatory system.
  • the oligomers of oligosaccharide of the present invention provide an advantage as being the delivery vehicle for sustained or slow release of GAG components. Another advantage is that the oligomers of monosaccharides are metabolized more slowly compared to the monomers of monosaccharides. In this regard, the uptake of the raw components is reduced since the metabolic losses through urine, feces, breath and perspiration is less significant. There are multiple enzyme activities in the body fluids that can biodegrade the oligomers of monosaccharides and provide a sustained or slow release format for monomers to the different connective tissues and articular cartilage. The molecular ranges and sizes of the molecule uptake are important for the bioavailability and delivery kinetics.
  • a method of delivery into organism monomers of NAG and/or GS by the administration of short tandems containing between 2 to 100 units of NAG, monosaccharides, and/or GS in different proportions It is intended with the invention to obtain targeted physiological effects by administering selected length of tandems of monosaccharides.
  • the oligomers of the present invention comprise between about 2 to 25 saccharides.
  • other units of saccharides may consists of galactosamine, N-acetyl-galactosamine, galactose, glucuronate and iduronate, all products deriving from polysaccharides comprising among others, hyaluronan, chondroitin, dermatan and keratan.
  • One embodiment of the present invention is that administration or topical application of selected lengths of glucosamine or saccharide prodrugs may results in several targeted remedi health effects. Among these effects may be included a regenerative effect, most particularly for articular cartilage. Also, specific lengths of oligomers of monosaccharides may have a beneficial effect on mucosal membranes by inhibiting the inflammatory processes, as in the case of Crohn's disease, ulcerative colitis, or elsewhere by immunomodulating specific pathways of the immune system. Other length of oligomers of saccharides may have static or lethal effects on microorganisms, including bacteria and fungi.
  • oligomers of saccharides of the present invention is intended to allow modulated release of GS and/or NAG at a rate proportional to the length of the oligomers.
  • endogenous enzymes lysosyme, chitinase, N-acetyl-D-glucosaminidase of a treated organism, a human for example, the units of GS and/or NAG, or very small repetitions of GS and/or NAG comprising, for example but not limited to, 2 to 5 units, may be released as the oligosaccharides are digested.
  • Another embodiment of the invention is a mean by which GS (and in a lesser means NAG) supplementation helps to reduce and stop the degenerative aspects of the ailments.
  • GS used for biosupplementation and chondrogenesis stimulation is normally supplied in the salt forms with the chloride or sulfate ions and administered orally by way of tablets, capsules or powders.
  • the glucosamine monomer active represents approximately 83% and 63% respectively of the total weight of the dried content.
  • the usually recommended daily dosage ranges from 1.5 to 3.0 grams/day (3 to 6 capsules of 500mg).
  • NAG is as a source of amino sugar for the synthesis of molecules such as glycoproteins and glycosaminoglycans, which are rich in NAG and the synthesis of which is stimulated by NAG.
  • Another embodiment of the present invention is that an advantageous prolonged and sustained or slow release of monomers or short oligosaccharide chains can be achieved during in vivo or in situ digestion of oligomers of monosaccharides administered into or topically applied on a body.
  • compositions containing the oligomers of the present invention may contain excipients adapted to all the forms used in creams or gels in pots or in tubes, milks, body emulsions, lotions in glass or plastic bottles and optionally in measuring bottles or also in jars, liquid soaps or bars.
  • Another object of the invention is to provide a dietary food supplement containing polysaccharides derivative oligosaccharides and an appetite modulating substance together with a nutriceutical to burn or better metabolize whatever fat the body does consume.
  • the polysaccharide sources of the invention may be characterized as to the proportion of N-acetyl-D-glucosamine units and D-glucosamine units, and such is expressed as the degree of deacetylation of the fully acetylated precursor.
  • the degree of deacetylation ranges preferably from 60% to 100%, more preferably from 90 to 100%, meaning that the proportion of N-acetyl-D-glucosamine units and D-glucosamine units in the oligomer is 0% to 40% of N-acetyl-D-glucosamine units and 60% to 100% of D-glucosamine units.
  • oligosaccharides are the deacetylated chitin and its derivative chitosan, other sources as hyaluronan, chondroitin, dermatan, keratan and derivatives thereof, may be considered in the present invention.
  • the physiologically functional derivatives also include prodrugs of the compounds according to the invention.
  • Such prodrugs can be metabolized in vivo to give a compound according to the invention.
  • One embodiment of the present invention is to provide a method to release biologically active units of glucosamine including them as part of a soluble prodrug or oligomer of it.
  • Oligomer prodrugs include chemical native or derivatives of a biologically active sub-unit, which, upon administration, eventually liberate the sub-unit in vivo.
  • Oligomer prodrugs of the present invention allow modifying the onset and/or duration of action of a sub-unit in vivo and can modify the transportation, distribution or solubility of a drug, the sub-unit, in the body.
  • oligomer prodrug formulations often reduce the toxicity and/or otherwise overcome difficulties encountered when administering pharmaceutical preparations.
  • Oligomer prodrugs are often biologically inert or substantially inactive forms of the parent or active compound.
  • a total of nine (9) male Beagle dogs were used during this study.
  • the animals were assigned to three treatment groups of 3 animals/group.
  • Group 1 received low dose Products X, Y and Glucosamine by oral administration on Days 1, 8 and 15, respectively.
  • Group 2 animals received high dose Products X and Y by oral administration on Days 1 and 8, respectively, and
  • Group 3 animals received low dose Products X and Y by intravenous injection on Days 1 and 8, respectively.
  • Low and high dose animals were administered nominal dose levels of 21.4 mg/kg and 214 mg/kg, respectively. These levels approximated lx and lOx the standard daily oral glucosamine consumption (1500 mg) for an average human (70 kg standard body weight).
  • the commercial glucosamine was assumed to contain 100% active ingredient.
  • a serum sample was collected from all animals in all dose groups prior to dosing.
  • glucosamine peaked at a group mean of 977 ⁇ 273 ng/mL and 2810 ⁇ 394 ng/mL in the 21.4 and 214 mg/kg dose groups, respectively, but had decreased somewhat to 761+315.5 ng/mL and 2153 ⁇ 452 ng/mL by 120 minutes post-dose (Figs. 1 and 2).
  • glucosamine administration of 21.4 mg/kg led to a peak mean serum glucosamine of 1337 ng/mL at 60 minutes and a rapid decline to 533 ng/mL by 120 minutes post-dose (Fig. 1).
  • glucosamine levels showed another pattern, with peak levels achieved anywhere from 30 to 120 minutes post-dose in the individual animals with mean levels of about 300 ng/mL and 800 mg/mL in the 21.4 and 214 mg/kg dose groups, respectively (Figs. 1 and 2).
  • the pattern of serum glucosamine levels indicate a smaller, but more sustained absorption of glucosamine into the blood in the 2 hours following administration of Product X and Y, when compared to the commercial preparation of glucosamine.
  • the review of the raw peak and AUC data shows that glucosamine levels are lower following Products X and Y, indicating less bioavailability over the first 2 hours after dosing.
  • glucosamine levels do not decrease as rapidly from peak levels following Products X and Y, indicating that higher serum levels are maintained for a longer period of time when compared to commercial glucosamine.
  • a comparison of the peak levels of serum glucosamine obtained for the low and high dose groups shows a 2.5-fold increase for both Product X and Y, despite a 10-fold increase in the dose administered.
  • This non- linearity in the bioavailability of glucosamine from oral administration of Products X and Y may be due to saturation of absorption or catabolic pathways in the gut.
  • Intravenous Administration Mean peak levels following intravenous administration were 458 ⁇
  • Oral and intravenous administration of Product X and Y were found to increase serum glucosamine levels in the acute post-dose period (0- 2 hours). Oral administration of Product X delivered about 3-fold more glucosamine than Product Y in the 2 hours immediately following dosing.
  • the data shows that oral and intravenous administration of both Products is associated with sustained elevations of serum glucosamine for up to 8-12 hours.

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Abstract

L'invention concerne un procédé de libération contrôlée de saccharides et d'oligosaccharides chez l'être humain et des animaux. Les polysaccharides sont digérés de manière à obtenir des oligomères ayant des nombres souhaités d'unités de saccharides ou de monosaccharides, notamment la glucosamine et la N-acétyl-glucosamine et des dérivés de ces dernières. Le taux de libération de monosaccharides est proportionnel à la longueur des oligomères administrés à un organisme et entraîne des effets physiologiques ciblés qui dépendent de la longueur des oligomères utilisés.
PCT/CA2002/001917 2001-12-13 2002-12-12 Procede permettant de moduler la liberation de saccharides et utilisations dudit procede WO2003054208A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US10/498,002 US20050070500A1 (en) 2001-12-13 2002-12-12 Method of modulating release of saccharides and uses thereof
EP02787250A EP1455835A2 (fr) 2001-12-13 2002-12-12 Procede permettant de moduler la liberation de saccharides et utilisations dudit procede
AU2002351576A AU2002351576A1 (en) 2001-12-13 2002-12-12 Method of modulating release of saccharides and uses thereof
CA002507870A CA2507870A1 (fr) 2001-12-13 2002-12-12 Procede permettant de moduler la liberation de saccharides et utilisations dudit procede

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US33933901P 2001-12-13 2001-12-13
US60/339,339 2001-12-13

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WO2003054208A2 true WO2003054208A2 (fr) 2003-07-03
WO2003054208A3 WO2003054208A3 (fr) 2003-10-09

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US20050070500A1 (en) 2005-03-31
EP1455835A2 (fr) 2004-09-15
WO2003054208A3 (fr) 2003-10-09
AU2002351576A1 (en) 2003-07-09
CA2507870A1 (fr) 2003-07-03
AU2002351576A8 (en) 2003-07-09

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