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WO2003052084A3 - Construits cellulaires cultives in vitro, preparation et utilisations - Google Patents

Construits cellulaires cultives in vitro, preparation et utilisations Download PDF

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Publication number
WO2003052084A3
WO2003052084A3 PCT/FR2002/004360 FR0204360W WO03052084A3 WO 2003052084 A3 WO2003052084 A3 WO 2003052084A3 FR 0204360 W FR0204360 W FR 0204360W WO 03052084 A3 WO03052084 A3 WO 03052084A3
Authority
WO
WIPO (PCT)
Prior art keywords
vitro
cells
cultured
preparation
tissue
Prior art date
Application number
PCT/FR2002/004360
Other languages
English (en)
Other versions
WO2003052084A2 (fr
Inventor
Annie Black
Jean-Louis Romette
Original Assignee
Natural Implant
Annie Black
Jean-Louis Romette
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Natural Implant, Annie Black, Jean-Louis Romette filed Critical Natural Implant
Priority to EP02804924A priority Critical patent/EP1453955A2/fr
Priority to US10/498,264 priority patent/US20050053585A1/en
Priority to IL16234302A priority patent/IL162343A0/xx
Priority to AU2002364653A priority patent/AU2002364653B2/en
Priority to BR0214860-9A priority patent/BR0214860A/pt
Priority to JP2003552951A priority patent/JP2005512541A/ja
Priority to CA002469611A priority patent/CA2469611A1/fr
Publication of WO2003052084A2 publication Critical patent/WO2003052084A2/fr
Publication of WO2003052084A3 publication Critical patent/WO2003052084A3/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3633—Extracellular matrix [ECM]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A61L27/386—Ligaments, tendons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A61L27/3865—Dental/periodontal tissues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/066—Tenocytes; Tendons, Ligaments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/10—Mineral substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/10—Mineral substrates
    • C12N2533/18—Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Botany (AREA)
  • Public Health (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Rheumatology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Rehabilitation Therapy (AREA)
  • Vascular Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un construit cellulaire cultivé in vitro caractérisé en ce qu'il comprend (i) des cellules animales cultivées in vitro dans des conditions assurant la formation d'une structure tissulaire tridimensionnelle et (ii) une matrice extra-cellulaire endogène dans laquelle lesdites cellules sont emprisonnées, et en ce qu'il comprend, parmi lesdites cellules, des cellules ayant une capacité de minéralisation et/ou d'ossification. L'invention est particulièrement utile dans le domaine des greffes ou de l'implantologie (reconstitution ou réparation tissulaire), ainsi que dans l'industrie pharmaceutique, pour l'étude des tissus et l'analyse du profil toxique ou bénéfique de molécules susceptibles d'entrer en développement pharmaceutique et/ou dans des compositions pharmaceutiques.
PCT/FR2002/004360 2001-12-14 2002-12-13 Construits cellulaires cultives in vitro, preparation et utilisations WO2003052084A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP02804924A EP1453955A2 (fr) 2001-12-14 2002-12-13 Construits cellulaires cultives in vitro, preparation et utilisations
US10/498,264 US20050053585A1 (en) 2001-12-14 2002-12-13 Cell constructs cultured in vitro, preparation and uses
IL16234302A IL162343A0 (en) 2001-12-14 2002-12-13 Cell constructs cultured in vitro, preparation anduses
AU2002364653A AU2002364653B2 (en) 2001-12-14 2002-12-13 Cell constructs cultured in vitro, preparation and uses
BR0214860-9A BR0214860A (pt) 2001-12-14 2002-12-13 Construto ou tecido celular, processo de preparação de um construto celular, utilização de um construto, método de preparação de um implante com ajuda de um construto, kit para a preparação de um construto, composição farmacêutica e implante
JP2003552951A JP2005512541A (ja) 2001-12-14 2002-12-13 インビトロ培養した細胞構築物、その調製および使用
CA002469611A CA2469611A1 (fr) 2001-12-14 2002-12-13 Construits cellulaires cultives in vitro, preparation et utilisations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FRFR0116265 2001-12-14
FR0116265A FR2833610B1 (fr) 2001-12-14 2001-12-14 Construits cellulaires cultives in vitro, preparation et utilisations

Publications (2)

Publication Number Publication Date
WO2003052084A2 WO2003052084A2 (fr) 2003-06-26
WO2003052084A3 true WO2003052084A3 (fr) 2003-12-11

Family

ID=8870550

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/004360 WO2003052084A2 (fr) 2001-12-14 2002-12-13 Construits cellulaires cultives in vitro, preparation et utilisations

Country Status (10)

Country Link
US (1) US20050053585A1 (fr)
EP (1) EP1453955A2 (fr)
CN (1) CN1604962A (fr)
AU (1) AU2002364653B2 (fr)
BR (1) BR0214860A (fr)
CA (1) CA2469611A1 (fr)
FR (1) FR2833610B1 (fr)
IL (1) IL162343A0 (fr)
PL (1) PL370703A1 (fr)
WO (1) WO2003052084A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2416801C (fr) * 2000-07-21 2012-01-03 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Cellules souches humaines, adultes, de la pulpe dentaire in vitro et in vivo
ES2540242T3 (es) * 2003-08-01 2015-07-09 Cellseed Inc. Estructura tridimensional de tejido
JP4827729B2 (ja) * 2004-04-25 2011-11-30 株式会社セルシード 培養歯根膜細胞シート、製造方法及びその利用方法
ATE499435T1 (de) * 2005-09-30 2011-03-15 Global Dental Aps Verfahren zur differenzierung von stammzellen und verwendung davon bei der behandlung von zahnleiden
RU2498808C9 (ru) 2006-03-03 2014-01-20 Огенодженесис, Инк. Способ лечения состояния ротовой полости больного (варианты)
US8097455B2 (en) * 2007-02-02 2012-01-17 The Regents Of The University Of Michigan System and method for forming skeletal muscle constructs having functional tissue interfaces
CN101134946B (zh) * 2007-08-03 2011-12-21 陆洪光 一种黑色素瘤体外模型的制作方法
WO2009020651A2 (fr) * 2007-08-08 2009-02-12 Pervasis Therapeutics, Inc. Matériaux et procédés pour traiter un dommage causé au système squelettique et favoriser une réparation et une régénération du système squelettique
WO2009066283A2 (fr) * 2007-11-19 2009-05-28 Ben Gurion University Of The Negev Research And Development Authority Effets du corail à médiation par le calcium et procédés d'utilisation correspondants
WO2009151678A1 (fr) * 2008-03-11 2009-12-17 Massachusetts Institute Of Technology Surfaces sensibles aux stimuli
DE102008049014A1 (de) * 2008-09-25 2010-04-22 Bagambisa, Frank, Dr. Dentalimplantat zur Implantation in einen menschlichen oder tierischen Kieferknochen und Verfahren zu seiner Herstellung
CN102068318B (zh) * 2010-12-21 2012-11-21 四川大学 生物牙根支架材料的构建方法
CN103611193B (zh) * 2013-11-26 2015-05-27 中山大学 一种碳酸钙微球-细胞外基质复合材料及其制备方法和应用
US20180303974A1 (en) 2015-11-03 2018-10-25 Ariel-University Research And Development Company Ltd. Compositions for regeneration and repair of neural tissue

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WO2000069355A1 (fr) * 1999-05-14 2000-11-23 Gregory Altman Ligament croise anterieur fabrique par bio-ingenierie

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WO1998022154A2 (fr) * 1996-11-21 1998-05-28 Tissue Engineering, Inc. Mousses de biopolymere pour la reparation et la reconstruction de tissus
WO2000069355A1 (fr) * 1999-05-14 2000-11-23 Gregory Altman Ligament croise anterieur fabrique par bio-ingenierie

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BHATNAGAR R.S. ET AL.: "Design of biomimetic habitats for tissue engineering with P-15, a synthetic peptide analogue of collagen", TISSUE ENGINEERING, vol. 5, no. 1, February 1999 (1999-02-01), pages 53 - 65, XP001040569 *
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Also Published As

Publication number Publication date
BR0214860A (pt) 2004-12-14
AU2002364653A1 (en) 2003-06-30
CN1604962A (zh) 2005-04-06
EP1453955A2 (fr) 2004-09-08
CA2469611A1 (fr) 2003-06-26
US20050053585A1 (en) 2005-03-10
IL162343A0 (en) 2005-11-20
FR2833610B1 (fr) 2007-01-26
PL370703A1 (en) 2005-05-30
FR2833610A1 (fr) 2003-06-20
AU2002364653B2 (en) 2007-10-18
WO2003052084A2 (fr) 2003-06-26

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