+

WO2003050501A3 - Indirect fluctuation screening - Google Patents

Indirect fluctuation screening Download PDF

Info

Publication number
WO2003050501A3
WO2003050501A3 PCT/US2002/025261 US0225261W WO03050501A3 WO 2003050501 A3 WO2003050501 A3 WO 2003050501A3 US 0225261 W US0225261 W US 0225261W WO 03050501 A3 WO03050501 A3 WO 03050501A3
Authority
WO
WIPO (PCT)
Prior art keywords
indirect
outside
cells
products
activity
Prior art date
Application number
PCT/US2002/025261
Other languages
French (fr)
Other versions
WO2003050501A2 (en
Inventor
Andrei V Gudkov
Original Assignee
Univ Illinois
Andrei V Gudkov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Illinois, Andrei V Gudkov filed Critical Univ Illinois
Priority to AU2002365031A priority Critical patent/AU2002365031A1/en
Publication of WO2003050501A2 publication Critical patent/WO2003050501A2/en
Publication of WO2003050501A3 publication Critical patent/WO2003050501A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention describes a general method of isolation genetic elements, encoding bioproducts that exert their biological activity from the outside the target cell. The method allows the effective identification of the desired gene products (and the factors induced by the activity of such products in target cells) that can act from the outside of the cells and, therefore, do not require additional delivery systems for their in vivo applications.
PCT/US2002/025261 2001-08-10 2002-08-08 Indirect fluctuation screening WO2003050501A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002365031A AU2002365031A1 (en) 2001-08-10 2002-08-08 Indirect fluctuation screening

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31166001P 2001-08-10 2001-08-10
US60/311,660 2001-08-10

Publications (2)

Publication Number Publication Date
WO2003050501A2 WO2003050501A2 (en) 2003-06-19
WO2003050501A3 true WO2003050501A3 (en) 2004-02-26

Family

ID=23207894

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/025261 WO2003050501A2 (en) 2001-08-10 2002-08-08 Indirect fluctuation screening

Country Status (2)

Country Link
AU (1) AU2002365031A1 (en)
WO (1) WO2003050501A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190382711A1 (en) * 2017-01-26 2019-12-19 Biomillenia Sas High-throughput microfluidic screening in droplets

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995023855A2 (en) * 1994-03-02 1995-09-08 Board Of Trustees Of The University Of Illinois Genes and genetic elements associated with control of neoplastic transformation in mammalian cells
US5665550A (en) * 1990-10-19 1997-09-09 Board Of Trustees Of The University Of Illinois-Urbana Genes and genetic elements associated with sensitivity to chemotherapeutic drugs
US6326488B1 (en) * 1990-10-19 2001-12-04 Board Of Trustees Of University Of Illinois Gene and genetic elements associated with sensitivity to chemotherapeutic drugs

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665550A (en) * 1990-10-19 1997-09-09 Board Of Trustees Of The University Of Illinois-Urbana Genes and genetic elements associated with sensitivity to chemotherapeutic drugs
US6326488B1 (en) * 1990-10-19 2001-12-04 Board Of Trustees Of University Of Illinois Gene and genetic elements associated with sensitivity to chemotherapeutic drugs
WO1995023855A2 (en) * 1994-03-02 1995-09-08 Board Of Trustees Of The University Of Illinois Genes and genetic elements associated with control of neoplastic transformation in mammalian cells

Also Published As

Publication number Publication date
AU2002365031A1 (en) 2003-06-23
WO2003050501A2 (en) 2003-06-19
AU2002365031A8 (en) 2003-06-23

Similar Documents

Publication Publication Date Title
WO2004024917A3 (en) Single-molecule in vitro evolution
EP1522581A3 (en) Optical sortingmethod
WO2004046160A8 (en) Amino-lna, thio-lna and alpha-l-oxy-ln
WO2003093306A3 (en) Nucleic acids and proteins from streptococcus groups a & b
WO1999036553A3 (en) Regulation of biological events using multimeric chimeric proteins
EP1801214A3 (en) In vitro sorting method
EP1300468A3 (en) TRNA synthetase
WO1999055858A3 (en) Human nucleic acid sequences obtained from pancreas tumor tissue
WO2002095051A3 (en) Mage-a3 peptides presented by hla class ii molecules
AU2002324909A1 (en) Linear dna fragments for gene expression
EP1624065A3 (en) Agarase and gene thereof
WO2000040695A3 (en) Sequence-determined dna fragments and corresponding polypeptides encoded thereby
WO2003050501A3 (en) Indirect fluctuation screening
WO2008152446A3 (en) Novel glycosylated peptide target in neoplastic cells
WO2005079367A3 (en) Schizochytrium fatty acid synthase (fas) and products and methods related thereto
WO2022056041A3 (en) Rna and dna base editing via engineered adar
WO1999039009A8 (en) Processes for isolating, amplifying and characterizing dna
WO2001021839A3 (en) Gene expression profiles
WO2002008266A3 (en) Complete nucleotide sequence of staphylococcus aureus ribosomal protein s16 gene and methods for the identification of antibacterial substances
WO2007014951A3 (en) Methods for the screening of antibacterial substances
WO2003066879A3 (en) Amino acid transporters
WO2004013608A3 (en) Methods for eliminating false data from comparative data matrices and for quantifying data matrix quality
WO2002008265A3 (en) Staphylococcus aureus ribosomal protein s20, corresponding gene and methods for the identification of antibacterial substances
WO2000066735A3 (en) Human ion channel proteins
CA2375549A1 (en) Delivery system for biological material

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载