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WO2003050265A3 - Compositions and methods for normalizing assays - Google Patents

Compositions and methods for normalizing assays Download PDF

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Publication number
WO2003050265A3
WO2003050265A3 PCT/US2002/039671 US0239671W WO03050265A3 WO 2003050265 A3 WO2003050265 A3 WO 2003050265A3 US 0239671 W US0239671 W US 0239671W WO 03050265 A3 WO03050265 A3 WO 03050265A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
assays
normalizing
compositions
enzyme activity
Prior art date
Application number
PCT/US2002/039671
Other languages
French (fr)
Other versions
WO2003050265A2 (en
Inventor
Justin Stege
Lori Preston
David Weiner
Original Assignee
Diversa Corp
Justin Stege
Lori Preston
David Weiner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diversa Corp, Justin Stege, Lori Preston, David Weiner filed Critical Diversa Corp
Priority to EP02795831A priority Critical patent/EP1453952A2/en
Priority to AU2002360565A priority patent/AU2002360565A1/en
Priority to CA002472054A priority patent/CA2472054A1/en
Priority to JP2003551287A priority patent/JP2005512526A/en
Publication of WO2003050265A2 publication Critical patent/WO2003050265A2/en
Publication of WO2003050265A3 publication Critical patent/WO2003050265A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

In one aspect, the invention provides compositions and methods for expression normalization of enzyme activity screens. Also provided are methods for method for normalizing gene expression in assays and high throughput enzymatic screens to measure enzyme activity. Also provided are novel inteins and nucleic acids encoding them.
PCT/US2002/039671 2001-12-10 2002-12-10 Compositions and methods for normalizing assays WO2003050265A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP02795831A EP1453952A2 (en) 2001-12-10 2002-12-10 Compositions and methods for normalizing assays
AU2002360565A AU2002360565A1 (en) 2001-12-10 2002-12-10 Compositions and methods for normalizing assays
CA002472054A CA2472054A1 (en) 2001-12-10 2002-12-10 Compositions and methods for normalizing assays
JP2003551287A JP2005512526A (en) 2001-12-10 2002-12-10 Compositions and methods for standardizing quantitation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33983701P 2001-12-10 2001-12-10
US60/339,837 2001-12-10

Publications (2)

Publication Number Publication Date
WO2003050265A2 WO2003050265A2 (en) 2003-06-19
WO2003050265A3 true WO2003050265A3 (en) 2004-02-19

Family

ID=23330827

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/039671 WO2003050265A2 (en) 2001-12-10 2002-12-10 Compositions and methods for normalizing assays

Country Status (5)

Country Link
EP (1) EP1453952A2 (en)
JP (1) JP2005512526A (en)
AU (1) AU2002360565A1 (en)
CA (1) CA2472054A1 (en)
WO (1) WO2003050265A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102712681A (en) * 2009-11-06 2012-10-03 谷万达公司 Intein-modified enzymes, their production and industrial applications

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2472886C (en) 2002-01-08 2013-05-28 R. Michael Raab Transgenic plants expressing civps or intein modified proteins and related method
CA2531494A1 (en) * 2003-07-11 2005-01-20 Novozymes A/S Method of screening for improved specific activity of enzymes
US9464333B2 (en) 2009-11-06 2016-10-11 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
US9249474B2 (en) 2009-11-06 2016-02-02 Agrivida, Inc. Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes
US10407742B2 (en) 2009-11-06 2019-09-10 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
US20130071884A1 (en) 2009-11-06 2013-03-21 Agrivida, Inc. Plants expressing cell wall degrading enzymes and expression vectors
US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
US10443068B2 (en) 2010-06-25 2019-10-15 Agrivida, Inc. Plants with engineered endogenous genes
CN111999272A (en) * 2020-08-13 2020-11-27 桂林医学院 Kanamycin detection method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020177691A1 (en) * 2001-03-20 2002-11-28 Scott Charles P. Trans inteins for protein domain shuffling and biopolymerization
US6562576B2 (en) * 2001-01-04 2003-05-13 Myriad Genetics, Inc. Yeast two-hybrid system and use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6562576B2 (en) * 2001-01-04 2003-05-13 Myriad Genetics, Inc. Yeast two-hybrid system and use thereof
US20020177691A1 (en) * 2001-03-20 2002-11-28 Scott Charles P. Trans inteins for protein domain shuffling and biopolymerization

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102712681A (en) * 2009-11-06 2012-10-03 谷万达公司 Intein-modified enzymes, their production and industrial applications
CN102712682B (en) * 2009-11-06 2016-07-06 谷万达公司 Intein modification enzyme and preparation thereof and industrial use
CN102712681B (en) * 2009-11-06 2016-07-06 谷万达公司 Intein modification enzyme and preparation thereof and industrial use

Also Published As

Publication number Publication date
JP2005512526A (en) 2005-05-12
CA2472054A1 (en) 2003-06-19
AU2002360565A1 (en) 2003-06-23
WO2003050265A2 (en) 2003-06-19
AU2002360565A8 (en) 2003-06-23
EP1453952A2 (en) 2004-09-08

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