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WO2003048363A1 - Complex of assigned molecule with c-end labeled protein, assigned molecule complex and methods of analyzing interaction between proteins using these complexes - Google Patents

Complex of assigned molecule with c-end labeled protein, assigned molecule complex and methods of analyzing interaction between proteins using these complexes Download PDF

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Publication number
WO2003048363A1
WO2003048363A1 PCT/JP2002/012864 JP0212864W WO03048363A1 WO 2003048363 A1 WO2003048363 A1 WO 2003048363A1 JP 0212864 W JP0212864 W JP 0212864W WO 03048363 A1 WO03048363 A1 WO 03048363A1
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WO
WIPO (PCT)
Prior art keywords
complex
assigned
molecule
interaction
proteins
Prior art date
Application number
PCT/JP2002/012864
Other languages
French (fr)
Japanese (ja)
Inventor
Etsuko Miyamoto
Hideaki Takashima
Hiroshi Yanagawa
Original Assignee
Keio University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keio University filed Critical Keio University
Priority to AU2002354116A priority Critical patent/AU2002354116A1/en
Priority to JP2003549540A priority patent/JP4030019B2/en
Publication of WO2003048363A1 publication Critical patent/WO2003048363A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A method of analyzing interaction between proteins with the use of an assigned molecule and a C-end labeled protein forming a complex, wherein the complex is formed by the interaction between the genotype/phenotype assigned molecule and the c-end labeled protein having a C-end labeling containing puromycin or its derivative, and part other than protein constituting the assigned molecule and the C-end labeling respectively have modifications required in analyzing the interaction. A method of analyzing interaction between proteins with the use of assigned molecules forming an assigned molecule complex which is a complex containing a plural number of genotype/phenotype assigned molecules and formed by the interaction among these genotype/phenotype assigned molecules and which is characterized by having modifications required in analyzing the interaction in parts other than proteins constituting the assigned molecules.
PCT/JP2002/012864 2001-12-07 2002-12-09 Complex of assigned molecule with c-end labeled protein, assigned molecule complex and methods of analyzing interaction between proteins using these complexes WO2003048363A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002354116A AU2002354116A1 (en) 2001-12-07 2002-12-09 Complex of assigned molecule with c-end labeled protein, assigned molecule complex and methods of analyzing interaction between proteins using these complexes
JP2003549540A JP4030019B2 (en) 2001-12-07 2002-12-09 Complex of mapping molecule and C-terminal labeled protein, complex of mapping molecule, and protein-protein interaction analysis method using the complex

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP2001374902 2001-12-07
JP2001-374902 2001-12-07
JP2001-374913 2001-12-07
JP2001374913 2001-12-07
JP2002172132 2002-06-12
JP2002-172132 2002-06-12

Publications (1)

Publication Number Publication Date
WO2003048363A1 true WO2003048363A1 (en) 2003-06-12

Family

ID=27347922

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/012864 WO2003048363A1 (en) 2001-12-07 2002-12-09 Complex of assigned molecule with c-end labeled protein, assigned molecule complex and methods of analyzing interaction between proteins using these complexes

Country Status (3)

Country Link
JP (1) JP4030019B2 (en)
AU (1) AU2002354116A1 (en)
WO (1) WO2003048363A1 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2003106675A1 (en) * 2002-06-12 2005-10-13 学校法人慶應義塾 Translation templates and libraries thereof, proteins and protein libraries synthesized from them, elements constituting them, and methods for producing and using them
JPWO2004113530A1 (en) * 2003-06-18 2006-08-03 三菱化学株式会社 Polynucleotide for labeling protein synthesis
JP2007282591A (en) * 2006-04-19 2007-11-01 Yamaguchi Univ Stathmin chip and method for detecting stathmin binding protein using the same
JPWO2005061706A1 (en) * 2003-11-19 2007-12-13 学校法人慶應義塾 Protein that forms a complex with c-Jun protein, nucleic acid encoding the same, and method of using the same
WO2011125833A1 (en) * 2010-03-31 2011-10-13 学校法人 慶應義塾 Constitution of tool for analyzing biomolecular interaction and analysis method using same
WO2013047785A1 (en) 2011-09-28 2013-04-04 国立大学法人東京大学 Method for detecting protein that interacts with target substance
WO2014080766A1 (en) * 2012-11-21 2014-05-30 国立大学法人埼玉大学 Nucleic acid linker

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11322781A (en) * 1998-05-15 1999-11-24 Mitsubishi Chemical Corp Protein labeling compound and protein labeling method using the compound
JP2000139468A (en) * 1998-11-11 2000-05-23 Mitsubishi Chemicals Corp Method for producing C-terminal labeled protein
JP2002176987A (en) * 2000-12-14 2002-06-25 Keio Gijuku Genotype-phenotype mapping molecules and their components, and methods for producing and using mapping molecules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11322781A (en) * 1998-05-15 1999-11-24 Mitsubishi Chemical Corp Protein labeling compound and protein labeling method using the compound
JP2000139468A (en) * 1998-11-11 2000-05-23 Mitsubishi Chemicals Corp Method for producing C-terminal labeled protein
JP2002176987A (en) * 2000-12-14 2002-06-25 Keio Gijuku Genotype-phenotype mapping molecules and their components, and methods for producing and using mapping molecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ETSUKO MIYAMOTO-SATO ET AL.: "Specific bonding of puromycin to full-length protein at the C-terminus", NUCLEIC ACIDS RESEARCH, vol. 28, no. 5, 2000, pages 1176 - 1182, XP002908838 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2003106675A1 (en) * 2002-06-12 2005-10-13 学校法人慶應義塾 Translation templates and libraries thereof, proteins and protein libraries synthesized from them, elements constituting them, and methods for producing and using them
JP4747292B2 (en) * 2002-06-12 2011-08-17 学校法人慶應義塾 Translation templates and libraries thereof, proteins and protein libraries synthesized from them, elements constituting them, and methods for producing and using them
JPWO2004113530A1 (en) * 2003-06-18 2006-08-03 三菱化学株式会社 Polynucleotide for labeling protein synthesis
JPWO2005061706A1 (en) * 2003-11-19 2007-12-13 学校法人慶應義塾 Protein that forms a complex with c-Jun protein, nucleic acid encoding the same, and method of using the same
US7838243B2 (en) 2003-11-19 2010-11-23 Keio University Protein forming complex with c-Jun protein, nucleic acid encoding the same and method of using the same
JP2007282591A (en) * 2006-04-19 2007-11-01 Yamaguchi Univ Stathmin chip and method for detecting stathmin binding protein using the same
WO2011125833A1 (en) * 2010-03-31 2011-10-13 学校法人 慶應義塾 Constitution of tool for analyzing biomolecular interaction and analysis method using same
JP5712382B2 (en) * 2010-03-31 2015-05-07 学校法人東京理科大学 Structure of biomolecular interaction analysis tool and analysis method using it
US9481882B2 (en) 2010-03-31 2016-11-01 Tokyo University Of Science Foundation Constitution of tool for analyzing biomolecular interaction and analysis method using same
WO2013047785A1 (en) 2011-09-28 2013-04-04 国立大学法人東京大学 Method for detecting protein that interacts with target substance
US9513295B2 (en) 2011-09-28 2016-12-06 Tokyo University Of Science Foundation Method for detecting protein that interacts with target substance
WO2014080766A1 (en) * 2012-11-21 2014-05-30 国立大学法人埼玉大学 Nucleic acid linker
JPWO2014080766A1 (en) * 2012-11-21 2017-01-05 国立大学法人埼玉大学 Nucleic acid linker

Also Published As

Publication number Publication date
JP4030019B2 (en) 2008-01-09
AU2002354116A1 (en) 2003-06-17
JPWO2003048363A1 (en) 2005-04-14

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