+

WO2003048344A3 - Differenciation de cellules souches embryonnaires humaines dans des embryons aviaires - Google Patents

Differenciation de cellules souches embryonnaires humaines dans des embryons aviaires Download PDF

Info

Publication number
WO2003048344A3
WO2003048344A3 PCT/IL2002/000978 IL0200978W WO03048344A3 WO 2003048344 A3 WO2003048344 A3 WO 2003048344A3 IL 0200978 W IL0200978 W IL 0200978W WO 03048344 A3 WO03048344 A3 WO 03048344A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
human embryonic
differentiation
embryonic stem
stem cells
Prior art date
Application number
PCT/IL2002/000978
Other languages
English (en)
Other versions
WO2003048344A2 (fr
Inventor
Ron Goldstein
Nissim Benvenisty
Micha Drukker
Original Assignee
Yissum Res Dev Co
Univ Bar Ilan
Ron Goldstein
Nissim Benvenisty
Micha Drukker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Res Dev Co, Univ Bar Ilan, Ron Goldstein, Nissim Benvenisty, Micha Drukker filed Critical Yissum Res Dev Co
Priority to IL16224902A priority Critical patent/IL162249A0/xx
Priority to EP02793287A priority patent/EP1458849A2/fr
Priority to US10/497,728 priority patent/US20050084960A1/en
Priority to AU2002358943A priority patent/AU2002358943A1/en
Publication of WO2003048344A2 publication Critical patent/WO2003048344A2/fr
Publication of WO2003048344A3 publication Critical patent/WO2003048344A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de préparation de cellules différenciées destinées à la transplantation à partir de cellules souches embryonnaires, par introduction de cellules souches embryonnaires humaines dans un embryon hôte aviaire. L'invention concerne également une méthode destinée à diriger la différenciation de cellules souches embryonnaires humaines par introduction de ces cellules au niveau d'une zone sélectionnée dans un embryon hôte aviaire, ce qui dicte leur modèle de différenciation. L'invention utilise des cellules humaines différenciées transplantables normales telles que des cellules progénitrices et d'autres cellules, et notamment des cellules neuronales. Ladite invention se rapporte en outre à des méthodes thérapeutiques et diagnostiques faisant appel aux cellules différenciées susmentionnées.
PCT/IL2002/000978 2001-12-07 2002-12-05 Differenciation de cellules souches embryonnaires humaines dans des embryons aviaires WO2003048344A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
IL16224902A IL162249A0 (en) 2001-12-07 2002-12-05 Differentiation of human embryonic stem cells in avian embryos
EP02793287A EP1458849A2 (fr) 2001-12-07 2002-12-05 Differenciation de cellules souches embryonnaires humaines dans des embryons aviaires
US10/497,728 US20050084960A1 (en) 2001-12-07 2002-12-05 Differentiation of human embryonic stem cells in avian embryos
AU2002358943A AU2002358943A1 (en) 2001-12-07 2002-12-05 Differentiation of human embryonic stem cells in avian embryos

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34098801P 2001-12-07 2001-12-07
US60/340,988 2001-12-07

Publications (2)

Publication Number Publication Date
WO2003048344A2 WO2003048344A2 (fr) 2003-06-12
WO2003048344A3 true WO2003048344A3 (fr) 2003-10-16

Family

ID=23335782

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2002/000978 WO2003048344A2 (fr) 2001-12-07 2002-12-05 Differenciation de cellules souches embryonnaires humaines dans des embryons aviaires

Country Status (5)

Country Link
US (1) US20050084960A1 (fr)
EP (1) EP1458849A2 (fr)
AU (1) AU2002358943A1 (fr)
IL (1) IL162249A0 (fr)
WO (1) WO2003048344A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060112438A1 (en) 2004-01-02 2006-05-25 West Michael D Novel culture systems for ex vivo development
WO2012106281A2 (fr) 2011-01-31 2012-08-09 The General Hospital Corporation Molécules trail multimodales et leurs utilisations en thérapies cellulaires
EP2877572B1 (fr) 2012-07-24 2018-11-28 The General Hospital Corporation Traitement par virus oncolytique de tumeurs résistantes
WO2014035474A1 (fr) 2012-08-30 2014-03-06 The General Hospital Corporation Compositions et méthodes de traitement du cancer
CN113558009B (zh) * 2021-07-07 2022-10-14 赛业（苏州）生物科技有限公司 一种使用es打靶技术获得的嵌合体鼠生殖遗传的挽救方法

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
EIGES RACHEL ET AL: "Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells.", CURRENT BIOLOGY, vol. 11, no. 7, 3 April 2001 (2001-04-03), pages 514 - 518, XP002228082, ISSN: 0960-9822 *
FONTAINE-PERUS JOSIANE ET AL: "Mouse-chick chimera: A developmental model of murine neurogenic cells.", DEVELOPMENT (CAMBRIDGE), vol. 124, no. 16, 1997, pages 3025 - 3036, XP002234871, ISSN: 0950-1991 *
GAJOVIC S ET AL: "DIFFERENTIATION OF THE MOUSE EMBRYOID BODIES GRAFTED ON THE CHORIOALLANTOIC MEMBRANE OF THE CHICK EMBRYO", INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, UNIVERSITY OF THE BASQUE COUNTRY PRES, LEIOA, ES, vol. 42, no. 2, March 1998 (1998-03-01), pages 225 - 228, XP001056263, ISSN: 0214-6282 *
GOLDSTEIN RONALD S ET AL: "Integration and differentiation of human embryonic stem cells transplanted to the chick embryo.", DEVELOPMENTAL DYNAMICS, vol. 225, no. 1, September 2002 (2002-09-01), September, 2002, pages 80 - 86, XP009007834, ISSN: 1058-8388 *
RATHJEN P D ET AL: "PROPERTIES AND USES OF EMBRYONIC STEM CELLS: PROSPECTS FOR APPLICATION TO HUMAN BIOLOGY AND GENE THERAPY", REPRODUCTION, FERTILITY AND DEVELOPMENT, CSIRO, EAST MELBOURNE, AU, vol. 10, no. 1, 1998, pages 31 - 47, XP000916997, ISSN: 1031-3613 *
TUAN R S ET AL: "Developmental Biology Protocols", METHODS IN MOLECULAR BIOLOGY, HUMANA PRESS INC., CLIFTON, NJ, US, vol. 1, 2000, pages 439 - 446, XP002198678 *

Also Published As

Publication number Publication date
IL162249A0 (en) 2005-11-20
US20050084960A1 (en) 2005-04-21
WO2003048344A2 (fr) 2003-06-12
EP1458849A2 (fr) 2004-09-22
AU2002358943A1 (en) 2003-06-17

Similar Documents

Publication Publication Date Title
EP2302034A3 (fr) Procédés améliorés de reprogrammation des cellules somatiques animales
NZ596425A (en) Materials and methods for regeneration and transformation of trees
WO2003085099A3 (fr) Procede de preparation de cellules souches provenant du tissu musculaire humain et du tissu adipeux, et cellules souches pouvant etre obtenues par ledit procede
EP3461327A3 (fr) Procédé et appareil permettant d'isoler globalement des tissus végétaux
WO2002064748A8 (fr) Cellules souches adultes totipotentes, sources de ces cellules, procedes d'obtention et de maintien de ces dernieres, procedes de differentiation de ces cellules, procedes d'utilisation correspondants et cellules derivees des cellules susmentionnees
PT1218509E (pt) Polipéptidos do tipo factor de crescimento de fibroblastos
GB2437027A (en) Differentiation of human embryonic stem cells and cardiomyocytes and cardiomyocyte progenitors derived therefrom
HU914086D0 (en) Method for explication of phytaze in plants
NO20092741L (no) DR6-antagonister og anvendelse derav ved behandling av neurologiske forstyrrelser
GB2407821A (en) Generation of neural stem cells from undifferentiated human embryonic stem cells
ATE539084T1 (de) Promotoren zur regulierung der genexpression in pflanzenwurzeln
Holder Positional information and pattern formation in plant morphogenesis and a mechanism for the involvement of plant hormones
WO2004018693A3 (fr) Procede de production de cetocarotenoides dans les petales de plantes
EP1865053A3 (fr) Procédé de modification de la morphologie, biochimie et physiologie des plantes comprenant l'expression d'une cytokinine oxydase des plantes
WO2003048344A3 (fr) Differenciation de cellules souches embryonnaires humaines dans des embryons aviaires
DE502005008759D1 (de) In vitro aus knochenmarkstammzellen differenzierteerwendung
WO2002098361A3 (fr) Reparation et regeneration de tissus induites par des cellules t
WO2003092611A3 (fr) Procedes pour induire la formation de vaisseaux lymphatiques fonctionnels et organises
WO2001072328A3 (fr) Methodes de traitement de maladies a l'aide de la proteine c activee
IL172499A0 (en) Method for producing retinal nerve cells from stem/progenitor cells derived from iris tissue and retinal nerve cells obtained by same method
WO2003033685A3 (fr) Procede destine a produire des lignees cellulaires beta humaines
AU2003298492A8 (en) Enzymatic method for the isolation of dna from plant tissue
DE69919989D1 (de) Verfahren zur herstellung transgener pflanzen unter verwendung von apikalspriessenspitzen
WO2004022707A3 (fr) Procedes et compositions utilises pour la transformation et la regeneration du mais
WO2006119965A3 (fr) Mutants du facteur de croissance aux attributs biologiques modifies

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 162249

Country of ref document: IL

WWE Wipo information: entry into national phase

Ref document number: 2002793287

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2002793287

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10497728

Country of ref document: US

WWW Wipo information: withdrawn in national office

Ref document number: 2002793287

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载