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WO2003040334A3 - Methods for the identification of inhibitors of 4-coumarate-coa ligase expression or activity in plants - Google Patents

Methods for the identification of inhibitors of 4-coumarate-coa ligase expression or activity in plants Download PDF

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Publication number
WO2003040334A3
WO2003040334A3 PCT/US2002/035596 US0235596W WO03040334A3 WO 2003040334 A3 WO2003040334 A3 WO 2003040334A3 US 0235596 W US0235596 W US 0235596W WO 03040334 A3 WO03040334 A3 WO 03040334A3
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WO
WIPO (PCT)
Prior art keywords
identification
activity
methods
coumarate
expression
Prior art date
Application number
PCT/US2002/035596
Other languages
French (fr)
Other versions
WO2003040334A2 (en
Inventor
Keith Davis
Adel Zayed
Robert Ascenzi
Douglas Boyes
Rao Mulpuri
Neil Hoffman
Kenneth Phillips
Jorn Gorlach
Jeffrey Woessner
Carol Hamilton
Original Assignee
Paradigm Genetics Inc
Keith Davis
Adel Zayed
Robert Ascenzi
Douglas Boyes
Rao Mulpuri
Neil Hoffman
Kenneth Phillips
Jorn Gorlach
Jeffrey Woessner
Carol Hamilton
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paradigm Genetics Inc, Keith Davis, Adel Zayed, Robert Ascenzi, Douglas Boyes, Rao Mulpuri, Neil Hoffman, Kenneth Phillips, Jorn Gorlach, Jeffrey Woessner, Carol Hamilton filed Critical Paradigm Genetics Inc
Priority to AU2002361590A priority Critical patent/AU2002361590A1/en
Publication of WO2003040334A2 publication Critical patent/WO2003040334A2/en
Publication of WO2003040334A3 publication Critical patent/WO2003040334A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present inventors have discovered that 4-coumarate-CoA ligase (4CL) is essential for plant growth. Specifically, the inhibition of 4CL gene expression in plant seedlings results in growth reduction and growth arrest. Thus, 4CL can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit 4CL expression or activity, comprising: contacting a compound with a 4CL and detecting the presence and/or absence of binding between said compound and said a 4CL, or detecting a decrease in 4CL expression or activity. The methods of the invention are useful for the identification of herbicides.
PCT/US2002/035596 2001-11-08 2002-11-06 Methods for the identification of inhibitors of 4-coumarate-coa ligase expression or activity in plants WO2003040334A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002361590A AU2002361590A1 (en) 2001-11-08 2002-11-06 Methods for the identification of inhibitors of 4-coumarate-coa ligase expression or activity in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33795501P 2001-11-08 2001-11-08
US60/337,955 2001-11-08

Publications (2)

Publication Number Publication Date
WO2003040334A2 WO2003040334A2 (en) 2003-05-15
WO2003040334A3 true WO2003040334A3 (en) 2003-11-20

Family

ID=23322753

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/035596 WO2003040334A2 (en) 2001-11-08 2002-11-06 Methods for the identification of inhibitors of 4-coumarate-coa ligase expression or activity in plants

Country Status (2)

Country Link
AU (1) AU2002361590A1 (en)
WO (1) WO2003040334A2 (en)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE PROTEIN [online] 18 August 2000 (2000-08-18), RIEGER M. ET AL., XP002963640, accession no. NCBI Database accession no. (T46131) *
EHLTING J. ET AL.: "Three 4-coumarate: coenzyme a ligases in arabidopsis thaliana represent two evolutionarily divergent classes in angiosperms", THE PLANT JOURNAL, vol. 19, no. 1, 1999, pages 9 - 20, XP002963639 *
VOO K.S. ET AL.: "4-Coumarate: coenzyme a ligase from loblolly pine xylem, isolation, characterization and complementary DNA cloning", PLANT PHYSIOLOGY, vol. 108, 1995, pages 85 - 97, XP002194496 *

Also Published As

Publication number Publication date
WO2003040334A2 (en) 2003-05-15
AU2002361590A1 (en) 2003-05-19

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