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WO2002038748A3 - Mammalian sex selection using genetic modification - Google Patents

Mammalian sex selection using genetic modification Download PDF

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Publication number
WO2002038748A3
WO2002038748A3 PCT/CA2001/001605 CA0101605W WO0238748A3 WO 2002038748 A3 WO2002038748 A3 WO 2002038748A3 CA 0101605 W CA0101605 W CA 0101605W WO 0238748 A3 WO0238748 A3 WO 0238748A3
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WO
WIPO (PCT)
Prior art keywords
meiotic
mediator
post
direct
male
Prior art date
Application number
PCT/CA2001/001605
Other languages
French (fr)
Other versions
WO2002038748A2 (en
Inventor
Steve Rothstein
Alan Wildeman
Original Assignee
Univ Guelph
Steve Rothstein
Alan Wildeman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Guelph, Steve Rothstein, Alan Wildeman filed Critical Univ Guelph
Priority to US10/416,417 priority Critical patent/US20040034879A1/en
Priority to CA002428326A priority patent/CA2428326A1/en
Priority to AU2002220392A priority patent/AU2002220392A1/en
Publication of WO2002038748A2 publication Critical patent/WO2002038748A2/en
Publication of WO2002038748A3 publication Critical patent/WO2002038748A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method of sex selection in non-human animals is disclosed which comprises transgenic manipulation of the male to modify, destroy or identify gametes having one of either the X or Y chromosome. A chimeric construct comprising a post-meiotic regulatory region in operative association with a sex selection gene is targeted to one of either a Y or X chromosome in the genome of a male animal. The post-meiotic regulatory region may be transition protein 1 promoter. The selection sequence may be a direct or indirect mediator, or a detectable marker. An example of a direct mediator is the Rnase, Barnase. An example of an indirect mediator is the herpes simplex virus thymidine kinase gene, which forms a toxic metabolite in the presence of gancyclovir. Alternatively, a transcriptional activator protein may be linked to the post-meiotic regulatory element, and the direct or indirect mediator may be linked to a promoter having a binding site specific for the transcriptional activator. According to the invention a non-human animal is formed which is capable of producing either exclusively male or exclusively female offspring, or a non-human animal is obtained from which X or Y containing gametes may be easily sorted and isolated. The invention is useful in applications which would benefit from the ability to obtain animals of a desired sex.
PCT/CA2001/001605 1999-11-09 2001-11-09 Mammalian sex selection using genetic modification WO2002038748A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/416,417 US20040034879A1 (en) 2000-11-09 2001-11-09 Mammalian sex selection using genetic modification
CA002428326A CA2428326A1 (en) 2000-11-09 2001-11-09 Mammalian sex selection using genetic modification
AU2002220392A AU2002220392A1 (en) 2000-11-09 2001-11-09 Mammalian sex selection using genetic modification

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16433399P 1999-11-09 1999-11-09
US70873400A 2000-11-09 2000-11-09
US09/708,734 2000-11-09

Publications (2)

Publication Number Publication Date
WO2002038748A2 WO2002038748A2 (en) 2002-05-16
WO2002038748A3 true WO2002038748A3 (en) 2003-08-07

Family

ID=26860460

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2001/001605 WO2002038748A2 (en) 1999-11-09 2001-11-09 Mammalian sex selection using genetic modification

Country Status (1)

Country Link
WO (1) WO2002038748A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0325140D0 (en) * 2003-10-28 2003-12-03 Babraham Inst Methods for selecting gametes and for producing genetically modified non-human animals
CN109402244B (en) * 2018-12-20 2022-05-03 广西大学 A method for identifying the sex of mammalian embryos

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5596089A (en) * 1994-02-14 1997-01-21 Universite De Montreal Oligonucleotide probe and primers specific to bovine or porcine male genomic DNA
WO2001062076A1 (en) * 2000-02-24 2001-08-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of mammals which produce progeny of a single sex

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5596089A (en) * 1994-02-14 1997-01-21 Universite De Montreal Oligonucleotide probe and primers specific to bovine or porcine male genomic DNA
WO2001062076A1 (en) * 2000-02-24 2001-08-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of mammals which produce progeny of a single sex

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
JOHNSON L A: "SEX PRESELECTION BY FLOW CYTOMETRIC SEPARATION OF X AND Y CHROMOSOME-BEARING SPERM BASED ON DNA DIFFERENCE: A REVIEW", REPRODUCTION, FERTILITY AND DEVELOPMENT, CSIRO, EAST MELBOURNE, AU, vol. 7, no. 4, 1995, pages 893 - 903, XP002915903, ISSN: 1031-3613 *
KISTLER M.K. ET AL.: "Promoter analysis of male germ cell-specific genes: nuclear transition protein 1 and histone H1t", 1996, IN: "CELLULAR AND MOLECULAR REGULATION OF TESTICULAR CELLS", C. DESJARDIN, ED., SPRINGER VERLAG (0-387-94648-9), XP008014158 *
LEE P. ET AL.: "Conditional lineage ablation to model human diseases", PROC. NATL. ACAD. SCI. USA, vol. 95, September 1998 (1998-09-01), pages 11371 - 11376, XP002233236 *
LEUCHTENBERGER S. ET AL.: "Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells.", NUCL. ACIDS RES., vol. 29, no. 16, 15 August 2001 (2001-08-15), pages e76, XP002233237, Retrieved from the Internet <URL:http://nar.oupjournals.org/cgi/reprint/29/16/e76.pdf> [retrieved on 20030303] *
YELICK P.C. ET AL.: "The mouse transition protein 1 gene contains a B1 repetitive element and is located on chromosome 1.", GENOMICS, vol. 11, 1991, pages 687 - 694, XP008014175 *

Also Published As

Publication number Publication date
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