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WO2002037077A3 - Screening methods based on transcriptional regulation analysis system using transformed cell lines - Google Patents

Screening methods based on transcriptional regulation analysis system using transformed cell lines Download PDF

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Publication number
WO2002037077A3
WO2002037077A3 PCT/KR2001/001881 KR0101881W WO0237077A3 WO 2002037077 A3 WO2002037077 A3 WO 2002037077A3 KR 0101881 W KR0101881 W KR 0101881W WO 0237077 A3 WO0237077 A3 WO 0237077A3
Authority
WO
WIPO (PCT)
Prior art keywords
screening methods
cell lines
transformed cell
analysis system
methods based
Prior art date
Application number
PCT/KR2001/001881
Other languages
French (fr)
Other versions
WO2002037077A2 (en
Inventor
Seok-Geun Lee
Mun-Seog Chang
Ho-Jin Shin
Hyune-Mo Rho
Original Assignee
Medexbio Co
Seok-Geun Lee
Mun-Seog Chang
Ho-Jin Shin
Hyune-Mo Rho
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medexbio Co, Seok-Geun Lee, Mun-Seog Chang, Ho-Jin Shin, Hyune-Mo Rho filed Critical Medexbio Co
Priority to AU2002215239A priority Critical patent/AU2002215239A1/en
Publication of WO2002037077A2 publication Critical patent/WO2002037077A2/en
Publication of WO2002037077A3 publication Critical patent/WO2002037077A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to screening methods for evaluating the activity of transcription factors or promoters using transformed cell lines. More particularly, the present invention relates to (a) screening methods for detection of substances affecting transcription activity by use of transformed cell lines, which leads to cell-based data with little artificial effects, (b) cell lines for performing the screening methods, and (c) screening methods for leading compounds for new drug.
PCT/KR2001/001881 2000-11-06 2001-11-06 Screening methods based on transcriptional regulation analysis system using transformed cell lines WO2002037077A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002215239A AU2002215239A1 (en) 2000-11-06 2001-11-06 Screening methods based on transcriptional regulation analysis system using transformed cell lines

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR2000/65656 2000-11-06
KR1020000065656A KR20020035393A (en) 2000-11-06 2000-11-06 Reporter Vectors for Evaluating the Activity of Transcription Factors or Promoters and Cell Lines Transformed with the same

Publications (2)

Publication Number Publication Date
WO2002037077A2 WO2002037077A2 (en) 2002-05-10
WO2002037077A3 true WO2002037077A3 (en) 2002-11-07

Family

ID=19697487

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2001/001881 WO2002037077A2 (en) 2000-11-06 2001-11-06 Screening methods based on transcriptional regulation analysis system using transformed cell lines

Country Status (3)

Country Link
KR (2) KR20020035393A (en)
AU (1) AU2002215239A1 (en)
WO (1) WO2002037077A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10987362B2 (en) 2004-03-12 2021-04-27 Intercept Pharmaceuticals, Inc. Treatment of fibrosis using FXR ligands
KR100711939B1 (en) * 2005-12-29 2007-05-02 한국화학연구원 Screening method using transformed cell line and recombinant virus
KR100783491B1 (en) * 2006-07-27 2007-12-07 고려대학교 산학협력단 Vector for measuring promoter activity, method for measuring promoter activity using the same and promoter selected by the method
KR101144885B1 (en) * 2007-11-09 2012-06-13 포항공과대학교 산학협력단 Dual biosensor for detecting estrogen, dioxin, and derivatives thereof and uses thereof
KR101428566B1 (en) * 2012-04-09 2014-08-11 한국생명공학연구원 Recombinant fluorescent translational reporter vector for detecting promoter strength or expression level of target protein and uses thereof
CN103305550B (en) * 2013-04-10 2015-06-17 重庆医科大学附属儿童医院 Reporter gene carrier for detecting activity of AP2alpha transcription factor
CN110885845A (en) * 2019-06-19 2020-03-17 中国农业科学院农业基因组研究所 A method for screening active enhancers using vectors based on random sequences
CN110257430A (en) * 2019-06-19 2019-09-20 中国农业科学院农业基因组研究所 A kind of active enhancer screening vector and its construction method
CN116286991B (en) * 2023-02-10 2023-10-13 中国农业科学院农业基因组研究所 Whole genome enhancer screening system, screening method and application

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997010360A1 (en) * 1995-09-13 1997-03-20 Chiron Corporation Method and construct for screening for inhibitors of transcriptional activation
WO1998003676A1 (en) * 1996-07-17 1998-01-29 Hagenmaier Hans Paul Process for detecting effectors of intra- and/or intercellular protein-protein interactions
US5976793A (en) * 1989-07-18 1999-11-02 Oncogene Science, Inc. Methods of transcriptionally modulating gene expression and of discovering chemicals capable as gene expression modulators
WO2000040720A1 (en) * 1999-01-08 2000-07-13 Sumitomo Chemical Company, Limited Cells for measuring ability to promote transcription

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976793A (en) * 1989-07-18 1999-11-02 Oncogene Science, Inc. Methods of transcriptionally modulating gene expression and of discovering chemicals capable as gene expression modulators
WO1997010360A1 (en) * 1995-09-13 1997-03-20 Chiron Corporation Method and construct for screening for inhibitors of transcriptional activation
WO1998003676A1 (en) * 1996-07-17 1998-01-29 Hagenmaier Hans Paul Process for detecting effectors of intra- and/or intercellular protein-protein interactions
WO2000040720A1 (en) * 1999-01-08 2000-07-13 Sumitomo Chemical Company, Limited Cells for measuring ability to promote transcription

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 200041, Derwent World Patents Index; AN 2000-475830/41 *

Also Published As

Publication number Publication date
KR20020035393A (en) 2002-05-11
WO2002037077A2 (en) 2002-05-10
AU2002215239A1 (en) 2002-05-15
KR20030065506A (en) 2003-08-06

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