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WO2002036815A3 - Genetic analysis of microorganisms - Google Patents

Genetic analysis of microorganisms

Info

Publication number
WO2002036815A3
WO2002036815A3 PCT/GB2001/004760 GB0104760W WO0236815A3 WO 2002036815 A3 WO2002036815 A3 WO 2002036815A3 GB 0104760 W GB0104760 W GB 0104760W WO 0236815 A3 WO0236815 A3 WO 0236815A3
Authority
WO
WIPO (PCT)
Prior art keywords
genetic analysis
microorganisms
taxonomic
linking
analysis
Prior art date
Application number
PCT/GB2001/004760
Other languages
French (fr)
Other versions
WO2002036815A2 (en
Inventor
Stephen Minter
James Ivor Prosser
Carol Jane Phillips
Original Assignee
Ncimb Ltd
Stephen Minter
James Ivor Prosser
Carol Jane Phillips
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ncimb Ltd, Stephen Minter, James Ivor Prosser, Carol Jane Phillips filed Critical Ncimb Ltd
Priority to GB0314840A priority Critical patent/GB2390159A/en
Priority to AU2002214126A priority patent/AU2002214126A1/en
Publication of WO2002036815A2 publication Critical patent/WO2002036815A2/en
Publication of WO2002036815A3 publication Critical patent/WO2002036815A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for genetic analysis of a microbial sample by linking taxonomic to functional characteristics comprises co-amplification of a taxonomic gene and a functional gene in a microbial sample and linking of the two amplified products. An analysis may then be performed for the linked product.
PCT/GB2001/004760 2000-10-28 2001-10-29 Genetic analysis of microorganisms WO2002036815A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB0314840A GB2390159A (en) 2000-10-28 2001-10-29 Genetic analysis of microorganisms
AU2002214126A AU2002214126A1 (en) 2000-10-28 2001-10-29 Genetic analysis of microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0026424.2 2000-10-28
GBGB0026424.2A GB0026424D0 (en) 2000-10-28 2000-10-28 Genetic analysis of microorganisms

Publications (2)

Publication Number Publication Date
WO2002036815A2 WO2002036815A2 (en) 2002-05-10
WO2002036815A3 true WO2002036815A3 (en) 2003-10-16

Family

ID=9902147

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/004760 WO2002036815A2 (en) 2000-10-28 2001-10-29 Genetic analysis of microorganisms

Country Status (3)

Country Link
AU (1) AU2002214126A1 (en)
GB (2) GB0026424D0 (en)
WO (1) WO2002036815A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2007081386A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP3335782B1 (en) 2006-05-11 2020-09-09 Bio-Rad Laboratories, Inc. Microfluidic devices
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
CA2775273A1 (en) * 2009-10-22 2011-04-28 Biotools Biotechnological & Medical Laboratories, S.A. Composition, method and kit for detecting bacteria by means of sequencing
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP5934657B2 (en) 2010-02-12 2016-06-15 レインダンス テクノロジーズ， インコーポレイテッド Digital specimen analysis
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
EP2673614B1 (en) 2011-02-11 2018-08-01 Raindance Technologies, Inc. Method for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US9556470B2 (en) 2011-06-02 2017-01-31 Raindance Technologies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
JP2015519900A (en) 2012-05-21 2015-07-16 フリューダイム・コーポレイション Single particle analysis method and single particle isolation method for particle population
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0628640A1 (en) * 1993-06-04 1994-12-14 Becton, Dickinson and Company Simultaneous amplification of multiple targets
US5614388A (en) * 1990-12-20 1997-03-25 Hoffmann-La Roche Inc. PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays
WO1998038298A1 (en) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Method for assembly of multiple dna fragments

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614388A (en) * 1990-12-20 1997-03-25 Hoffmann-La Roche Inc. PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays
EP0628640A1 (en) * 1993-06-04 1994-12-14 Becton, Dickinson and Company Simultaneous amplification of multiple targets
WO1998038298A1 (en) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Method for assembly of multiple dna fragments

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BLACKWOOD KYM S ET AL: "Evaluation of recA sequences for identification of Mycobacterium species.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 38, no. 8, August 2000 (2000-08-01), pages 2846 - 2852, XP002241675, ISSN: 0095-1137 *
COTTRELL MATTHEW T ET AL: "Selected chitinase genes in cultured and uncultured marine bacteria in the alpha- and gamma-subclasses of the proteobacteria.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 66, no. 3, March 2000 (2000-03-01), pages 1195 - 1201, XP002241674, ISSN: 0099-2240 *
HODSON ROBERT E ET AL: "In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 61, no. 11, 1995, pages 4074 - 4082, XP001146824, ISSN: 0099-2240 *
HORZ HANS-PETER ET AL: "Identification of major subgroups of ammonia-oxidizing bacteria in environmental samples by T-RFLP analysis of amoA PCR products.", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 39, no. 3, February 2000 (2000-02-01), pages 197 - 204, XP001146823, ISSN: 0167-7012 *
KULSKI JERZY K ET AL: "Use of a multiplex PCR to detect and identify Mycobacterium avium and M. intracellulare in blood culture fluids of AIDS patients.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 33, no. 3, 1995, pages 668 - 674, XP002241759, ISSN: 0095-1137 *
MORA DIEGO ET AL: "Identification of Pediococcus acidilactici and Pediococcus pentosaceus based on 16S rRNA and ldhD gene-targeted multiplex PCR analysis.", FEMS MICROBIOLOGY LETTERS, vol. 151, no. 2, 1997, pages 231 - 236, XP002241673, ISSN: 0378-1097 *
OSORIO C R ET AL: "Multiplex PCR assay for ureC and 16S rRNA genes clearly discriminates between both subspecies of Photobacterium damselae.", DISEASES OF AQUATIC ORGANISMS, vol. 40, no. 3, 20 April 2000 (2000-04-20), pages 177 - 183, XP009011163, ISSN: 0177-5103 *
TSENG H: "DNA CLONING WITHOUT RESTRICTION ENZYME AND LIGASE", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 27, no. 6, December 1999 (1999-12-01), pages 1240 - 1244, XP001015911, ISSN: 0736-6205 *

Also Published As

Publication number Publication date
GB2390159A (en) 2003-12-31
AU2002214126A1 (en) 2002-05-15
GB0314840D0 (en) 2003-07-30
WO2002036815A2 (en) 2002-05-10
GB0026424D0 (en) 2000-12-13

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