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WO2002036760A3 - Methods for expressing endogenous genes by restriction enzyme mediated integration - Google Patents

Methods for expressing endogenous genes by restriction enzyme mediated integration Download PDF

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Publication number
WO2002036760A3
WO2002036760A3 PCT/US2001/045045 US0145045W WO0236760A3 WO 2002036760 A3 WO2002036760 A3 WO 2002036760A3 US 0145045 W US0145045 W US 0145045W WO 0236760 A3 WO0236760 A3 WO 0236760A3
Authority
WO
WIPO (PCT)
Prior art keywords
restriction enzyme
methods
endogenous genes
expressing endogenous
enzyme mediated
Prior art date
Application number
PCT/US2001/045045
Other languages
French (fr)
Other versions
WO2002036760A2 (en
Inventor
Janine Lin
Debbie Yaver
Donald Foster
Richard Holly
Original Assignee
Novozymes Biotech Inc
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biotech Inc, Zymogenetics Inc filed Critical Novozymes Biotech Inc
Priority to AU2002227069A priority Critical patent/AU2002227069A1/en
Priority to EP01992773A priority patent/EP1352060A2/en
Publication of WO2002036760A2 publication Critical patent/WO2002036760A2/en
Publication of WO2002036760A3 publication Critical patent/WO2002036760A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to methods for producing a mutant mammalian cell by introducing into mammalian cells a nucleic acid construct and a restriction enzyme under conditions where the nucleic acid construct integrates into the genome of the mammalian cells at sites generated by the restriction enzyme.
PCT/US2001/045045 2000-10-30 2001-10-30 Methods for expressing endogenous genes by restriction enzyme mediated integration WO2002036760A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002227069A AU2002227069A1 (en) 2000-10-30 2001-10-30 Methods for expressing endogenous genes by restriction enzyme mediated integration
EP01992773A EP1352060A2 (en) 2000-10-30 2001-10-30 Methods for expressing endogenous genes by restriction enzyme mediated integration

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70217700A 2000-10-30 2000-10-30
US09/702,177 2000-10-30

Publications (2)

Publication Number Publication Date
WO2002036760A2 WO2002036760A2 (en) 2002-05-10
WO2002036760A3 true WO2002036760A3 (en) 2003-08-14

Family

ID=24820153

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/045045 WO2002036760A2 (en) 2000-10-30 2001-10-30 Methods for expressing endogenous genes by restriction enzyme mediated integration

Country Status (3)

Country Link
EP (1) EP1352060A2 (en)
AU (1) AU2002227069A1 (en)
WO (1) WO2002036760A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005078069A1 (en) * 2003-05-09 2005-08-25 Codegrok, Inc., Dba Auilix Biopharma Method for identifying cellular targets
CN105101979B (en) 2012-12-21 2021-10-08 安斯泰来再生医药协会 Method and composition for preparing platelets from pluripotent stem cells
WO2020196742A1 (en) * 2019-03-28 2020-10-01 公益財団法人実験動物中央研究所 Immunodeficient rodent

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999015650A1 (en) * 1997-09-26 1999-04-01 Athersys, Inc. Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999015650A1 (en) * 1997-09-26 1999-04-01 Athersys, Inc. Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRENNEMAN MARK ET AL: "Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 93, no. 8, 1996, 1996, pages 3608 - 3612, XP002233940, ISSN: 0027-8424 *
MANIVASAKAM PALANIYANDI ET AL: "Restriction enzymes increase efficiencies of illegitimate DNA integration but decrease homologous integration in mammalian cells.", NUCLEIC ACIDS RESEARCH, vol. 29, no. 23, 1 December 2001 (2001-12-01), December 1, 2001, pages 4826 - 4833, XP002233941, ISSN: 0305-1048 *
SHEMESH M ET AL: "Gene integration into bovine sperm genome and its expression in transgenic offspring.", MOLECULAR REPRODUCTION AND DEVELOPMENT. UNITED STATES JUN 2000, vol. 56, no. 2 Suppl, June 2000 (2000-06-01), pages 306 - 308, XP008014700, ISSN: 1040-452X *
YORIFUJI T ET AL: "CO-TRANSFER OF RESTRICTION ENDONUCLEASES AND PLASMID DNA INTO MAMMALIAN CELLS BY ELECTROPORATION EFFECTS ON STABLE TRANSFORMATION", MUTATION RESEARCH, vol. 243, no. 2, 1990, pages 121 - 126, XP008014769, ISSN: 0027-5107 *

Also Published As

Publication number Publication date
EP1352060A2 (en) 2003-10-15
WO2002036760A2 (en) 2002-05-10
AU2002227069A1 (en) 2002-05-15

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