WO2002034947A3 - Detecting specific nucleotide sequences - Google Patents
Detecting specific nucleotide sequences Download PDFInfo
- Publication number
- WO2002034947A3 WO2002034947A3 PCT/US2001/032630 US0132630W WO0234947A3 WO 2002034947 A3 WO2002034947 A3 WO 2002034947A3 US 0132630 W US0132630 W US 0132630W WO 0234947 A3 WO0234947 A3 WO 0234947A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sequence
- identifying
- specific
- representing
- nucleotide sequences
- Prior art date
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 2
- 239000013615 primer Substances 0.000 abstract 4
- 239000003155 DNA primer Substances 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 2
- 238000012408 PCR amplification Methods 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Oligonucleotide primers, each labeled with a molecular energy transfer pair, including an energy donor and an energy acceptor, are used to detect the presence of one or more identifying nucleotide sequences. Two sequence-specific oligonucleotide primers, one specific for a first identifying sequence and the other for the second identifying sequence, together with a reverse primer, are employed in a polymerase chain reaction mixture which contains a nucleic acid template. Using only its complementary identifying sequence as a template, each sequence-specific primer directs amplification. Resultant PCR products, representing the first and the second identifying sequence, are detected via PCR amplification which uses two MET-labeled primers, each specific for the PCR product representing either the first or the second identifying sequence, together with another primer that is complementary to the PCR products representing both identifying sequences.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2002213416A AU2002213416A1 (en) | 2000-10-24 | 2001-10-24 | Detecting specific nucleotide sequences |
| EP01981799A EP1354063A2 (en) | 2000-10-24 | 2001-10-24 | Detecting specific nucleotide sequences |
| JP2002537915A JP2004530410A (en) | 2000-10-24 | 2001-10-24 | Detection of specific nucleotide sequences |
| CA002426812A CA2426812A1 (en) | 2000-10-24 | 2001-10-24 | Detecting specific nucleotide sequences |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24267200P | 2000-10-24 | 2000-10-24 | |
| US60/242,672 | 2000-10-24 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2002034947A2 WO2002034947A2 (en) | 2002-05-02 |
| WO2002034947A3 true WO2002034947A3 (en) | 2003-08-14 |
Family
ID=22915737
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2001/032630 WO2002034947A2 (en) | 2000-10-24 | 2001-10-24 | Detecting specific nucleotide sequences |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1354063A2 (en) |
| JP (1) | JP2004530410A (en) |
| AU (1) | AU2002213416A1 (en) |
| CA (1) | CA2426812A1 (en) |
| WO (1) | WO2002034947A2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1419273A2 (en) * | 2001-08-21 | 2004-05-19 | The Secretary of State for the Home Department | Primers and method for the genotyping of snps by multiplex pcr |
| EP2975136A1 (en) * | 2014-07-14 | 2016-01-20 | Parlanca Limited | A method for detecting PCR amplification in a sample |
| WO2016008884A1 (en) | 2014-07-14 | 2016-01-21 | Identigen Limited | A method for detecting pcr amplification in a sample |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995013399A1 (en) * | 1993-11-12 | 1995-05-18 | The Public Health Research Institute Of The City Of New York, Inc. | Hybridization probes for nucleic acid detection, universal stems, methods and kits |
| WO1999029905A2 (en) * | 1997-12-12 | 1999-06-17 | Intergen Company | Method for detecting apoptosis using fret labeled oligonucleotides |
| US6090552A (en) * | 1996-07-16 | 2000-07-18 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
-
2001
- 2001-10-24 JP JP2002537915A patent/JP2004530410A/en active Pending
- 2001-10-24 EP EP01981799A patent/EP1354063A2/en not_active Withdrawn
- 2001-10-24 WO PCT/US2001/032630 patent/WO2002034947A2/en not_active Application Discontinuation
- 2001-10-24 AU AU2002213416A patent/AU2002213416A1/en not_active Abandoned
- 2001-10-24 CA CA002426812A patent/CA2426812A1/en not_active Abandoned
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995013399A1 (en) * | 1993-11-12 | 1995-05-18 | The Public Health Research Institute Of The City Of New York, Inc. | Hybridization probes for nucleic acid detection, universal stems, methods and kits |
| US6090552A (en) * | 1996-07-16 | 2000-07-18 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| WO1999029905A2 (en) * | 1997-12-12 | 1999-06-17 | Intergen Company | Method for detecting apoptosis using fret labeled oligonucleotides |
Non-Patent Citations (4)
| Title |
|---|
| HAWKINS J ROSS ET AL: "Miniaturized sealed-tube allele-specific PCR.", HUMAN MUTATION, vol. 19, no. 5, 2002, 2002, pages 543 - 553, XP002242293, ISSN: 1059-7794 * |
| MYAKISHEV MAXIM V ET AL: "High-throughput SNP genotyping by allele-specific PCR with universal energy-transfer-labeled primers.", GENOME RESEARCH, vol. 11, no. 1, January 2001 (2001-01-01), pages 163 - 169, XP002242292, ISSN: 1088-9051 * |
| NAZARENKO ET AL: "A CLOSED TUBE FORMAT FOR AMPLIFICATION AND DETECTION OF DNA BASED ENERGY TRANSFER", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 25, no. 12, 1997, pages 2516 - 2521, XP002094959, ISSN: 0305-1048 * |
| TYAGI S ET AL: "MOLECULAR BEACONS: PROBES THAT FLUORESCE UPON HYBRIDIZATION", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 14, 1 March 1996 (1996-03-01), pages 303 - 308, XP000196024, ISSN: 1087-0156 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002213416A1 (en) | 2002-05-06 |
| WO2002034947A2 (en) | 2002-05-02 |
| JP2004530410A (en) | 2004-10-07 |
| CA2426812A1 (en) | 2002-05-02 |
| EP1354063A2 (en) | 2003-10-22 |
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| 121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
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