WO2002034210A2 - Use of the association of at least a carotenoid and vitamin c for treating cutaneous symptoms of ageing - Google Patents

Abstract

The invention concerns the use in a composition or for preparing a composition of the association of at least a carotenoid and vitamin C, the association or the composition being designed to treat cutaneous symptoms of ageing, in particular degradation of the skin and/or of mucous membranes by inhibiting the activity and/or the expression of collagenases and by increasing collagen synthesis.

Description

Use of the combination of at least one carotenoid and vitamin C to treat the skin signs of aging

The invention relates to the use in a composition or for the preparation of a composition of the combination of at least one carotenoid and of vitamin C, the combination or the composition being intended to treat the cutaneous signs of aging. , in particular the degradation of the skin and / or mucous membranes by inhibiting the activity and / or expression of collagenases and by increasing the synthesis of collagen.

The invention also relates to a method of cosmetic treatment of the skin and / or mucous membranes.

In mammals in general, particularly in humans, the skin consists of two compartments, namely a compartment in relation to the outside, the epidemic, and n deep compartment which serves to support the epidermis, the dermis.

The epidermis is mainly composed of three types of cells which are the keratinocytes, very majority, the melanocytes and the Langerhans cells. Each of these cell types contributes by its own functions to the essential role played in the body by the skin.

The dermis provides the epidermis with solid support. It is also its nourishing element. It mainly consists of fibroblasts and an extracellular matrix itself composed mainly of collagen, elastin and a substance, known as the fundamental substance, components synthesized by the fibroblast. There are also leukocytes, mast cells or tissue macrophages, it is also crossed by blood vessels and nerve fibers. In normal skin, that is to say non-pathological or scarring, the fibroblast is in a quiescent state. ie non-proliferative, not very active from a metabolic point of view and not mobile

Collagen fibers largely ensure the solidity of the dermis. These fibers are made up of fibrils sealed to each other, thus forming more than ten different types of structures. The solidity of the dermis is largely due to the entanglement of collagen fibers packed against each other in all directions. Collagen fibers also participate in elasticity and especially in the tone of the skin and / or mucous membranes. The collagen fibers are constantly renewed, but this renewal decreases with age, which leads to thinning of the dermis. This thinning of the dermis is also due to pathological causes such as, for example, the hypersecretion of corticosteroid hormones, certain pathologies or vitamin deficiencies. It is also recognized that extrinsic factors such as ultraviolet rays, tobacco or certain treatments (Glucocorticoides, vitamin D and derivatives, for example) also have an effect on the skin and its collagen level.

However, various factors cause the degradation of collagen, with all the consequences that can be envisaged on the structure and / or firmness of the skin and / or mucous membranes.

Although very resistant, collagen fibers are sensitive to certain enzymes called collagenases. Degradation of collagen fibers results in the appearance of soft, wrinkled skin that has always been sought to combat, particularly in humans, preferring the appearance of smooth and taut skin.

Collagenases are part of a family of enzymes called metalloproteinases (MMPs) which are themselves members of a family of proteolytic enzymes (endoproteases) which have a zinc atom coordinated to 3 cysteine residues and a methionine in their active site and which degrade the macromolecular components of the extracellular matrix and basal laminae at neutral pH (collagen, etastin, etc.). Very widely distributed in the living world, these enzymes are present, but weakly expressed, in normal physiological situations such as your organ growth and tissue renewal.

Their overexpression and their activity are however linked to many processes, sometimes pathological, which involve the destruction and reshaping of the matrix. This either leads to an uncontrolled resorption of the extracellular matrix, or conversely the establishment of a state of fibrosis.

The family of metalloproteinases consists of several well-defined groups based on their similarities in terms of structure and specificity of substrate (see Woessner JF, Faseb Journal, vol. 5 1991, 2145). Among these groups, mention may be made of collagenases intended for degrading fibrillar collagens (MMP-1 or interstitial collagenase, MMP-8 or neutrophile collagenase, MMP-3 or collagenase 3), gelatinases which degrade collagen type IV or any form of denatured collagen (MMP-2 or gelatinase A (72 kDa), MMP-9 or gelatinase B (92 kDa)), stromelysins (MMP-3) whose broad spectrum of activity is address to proteins of the extracellular matrix such as glycoproteins (fibronectin, laminin), proteoglycans, etc., 5 or even membrane metalloproteinases

Prolonged exposure to ultraviolet radiation, particularly type A and / or B ultraviolet radiation, stimulates the expression of collagenases, particularly MMP-1. This is one of the components of photo-induced skin aging. t o

In addition to the menopause, the main modifications concerning the dermis are a reduction in the level of collagen and the dermal thickness, that involves in the menopausal woman a thinning of the skin and / or mucous membranes. The woman then feels a sensation of "dry skin" or of skin 15 which pulls and there is an accentuation of the wrinkles and fine lines on the surface. The skin has a rough appearance on the palpatîon. Finally, the skin has reduced flexibility.

It is then understood on reading the foregoing the importance of collagen in the structure of tissues, particularly of the skin and / or mucous membranes, and the importance of combating its degradation so as to fight against cutaneous signs of aging, whether chronological or photo-induced, and its consequences, such as, for example, thinning of the dermis and / or degradation of the collagen fibers, which cause the appearance of soft and wrinkled skin,

The expression “cutaneous signs of aging” means any modification of the external appearance of the skin due to aging, whether chronobiological and / or photo-induced, such as for example wrinkles and fine lines, withered skin, the skin

30 mole, thinned skin, dull and dull skin, lack of elasticity and / or tone of the skin, but also all internal modifications of the skin which do not systematically result in a modified external appearance, such as by example all internal degradations of the skin, particularly of collagen fibers. following exposure to ultraviolet radiation, which

35 can result in thinning of the dermis.

One of the aims of the present invention is therefore to be able to have a product which makes it possible to treat, in mammals in general, particularly in man, preventively and / or curatively, the skin signs of aging, whether chronobiological or photo-induced, particularly thinning of the dermis and / or degradation of collagen fibers, by a stimulating effect on synthesis collagen and / or a collagenase inhibiting effect and if possible no significant side effects.

Surprisingly, the Applicant has now discovered that the combination of at least one carotenoid and vitamin C makes it possible to treat, in a preventive and / or curative manner, the skin signs of aging, whether chronological or photo-induced, particularly thinning of the dermis and / or degradation of collagen fibers, by a stimulating effect on collagen synthesis and / or A collagenase inhibiting effect.

A remarkable property of the combination of the invention is that it has effects in greater proportions than those reasonably expected from the simple addition of the effects of each of these components taken separately.

An advantage of this property is to allow a use in the composition of the invention of a quantity of each of the products less than what is generally accepted to use,

To the knowledge of the applicant, the stimulatory activity on the synthesis of collagen and the inhibitory activity on the expression and / or the activity of collagenases from the combination of at least one carotenoid and vitamin C have never been described.

Thus, the first object of the invention is the use in a composition or for the preparation of a composition of the association of at least one carotenoid and of vitamin C, the association or the composition being intended to treat the signs skin of aging.

A second object of the invention is the use in a composition or for the preparation of a composition of the association of at least one carotenoid and of vitamin C, the association or the composition being intended to combat degradation of the collagen.

A third object of the invention is the use in a composition or for the preparation of a composition of the combination of at least one carotenoid and of vitamin C, the combination or the composition being intended to inhibit the expression of the proteases of the extracellular matrix, particularly metalloproteinases and even more particularly metalloproteinase type 1.

A fourth object of the invention is the use of the combination in a composition or for the preparation of a composition of the combination of at least one carotenoid and vitamin C, the combination or the composition being intended to treat skin disorders linked to aging, in particular skin disorders of menopause,

A fifth object of the invention is the use in a composition or for the preparation of a composition of the association of at least one carotenoid and of vitamin C, the association or the composition being intended to combat wrinkles and wrinkles.

The sixth object of the invention is the use in a composition or for the preparation of a composition of the association of at least one carotenoid and of vitamin C, the association or the composition being intended to fight against withered skin .

The seventh subject of the invention is the use in a composition or for the preparation of a composition of the association of at least one carotenoid and of vitamin C, the association or the composition being intended for combating soft skin .

The eighth object of the invention is the use in a composition or for the preparation of a composition of the association of at least one carotenoid and of vitamin C, the association or the composition being intended to fight against thinned skin. .

The ninth subject of the invention is the use in a composition or for the preparation of a composition of the association of at least one carotenoid and of vitamin C, the association or the composition being intended for combating dull skin and dull.

A tenth subject of the invention is the use in a composition or for the preparation of a composition of the combination of at least one carotenoid and of vitamin C, the combination or the composition being intended to combat the lack of elasticity and / or tone of the skin,

Whatever the intended use of the invention, it can be preventive and / or curative to the harm it aims to treat.

It is obvious that the invention is intended for mammals in general and particularly for human beings.

By carotenoid is meant according to the invention both a carotenoid with provitamin A activity, and a carotenoid without provitamin A activity.

Of course according to the invention, the carotenoid can be a mixture of carotenoids with provitamin A activity and carotenoids without provitamin A activity. This mixture can be in any proportion.

According to the invention, the carotenoid with provitamin A activity can be a mixture of carotenoids with provitamin A activity. This mixture can be in any proportion. Among the carotenoids with provitamin A activity, there may be mentioned by way of example β-carotene or α-carotene,

Preferably according to the invention, β-carotene is used.

According to the invention, the carotenoid without provitamin A activity can be a mixture of carotenoids without provitamin A activity. This mixture can be in any proportion. Among the carotenoids without provitamin A activity, mention may be made, for example, of zeaxanthin. cryptoxanthine, lutein or lycopene. Preferably according to the invention, lycopene is used.

Preferably according to the invention, a carotenoid without provitamin A activity is used and even more preferably lycopene.

Lycopene is a natural pigment found in ripe fruit, especially in tomatoes. It belongs to the carotenoid family and its structure is close to that of β-carotene. The role of lycopene in the ripening of fruits is known in the prior art Lycopene is used in compositions with bronzing activity for its role on the synthesis of melanin (WO 97/47278), in compositions intended for the treatment of the hair and / or acne for its activity on 5-reductases ( JP-2940964) or also as an anti-radical agent (JP-A- 8-283136.

Lycopene can be in cis or trans chemical form.

The carotenoid used according to the invention can be of natural or synthetic origin. By natural origin is meant the carotenoid, in the pure state or in solution whatever its concentration in said solution, obtained from a natural element.

According to a preferred embodiment of the invention, an extract rich in lycopene is used, such as for example a tomato extract. By synthetic origin is meant the carotenoid, in the pure state or in solution whatever its concentration in said solution, obtained by chemical synthesis.

When the carotenoid is of natural origin, it can be obtained from plant material from a whole plant grown in vivo or from in vitro culture.

The term “in vivo culture” means any culture of the conventional type, that is to say in soil in the open air or in a greenhouse, or even above ground.

The term “in vitro culture” means all of the techniques known to those skilled in the art which artificially make it possible to obtain a plant or part of a plant. The selection pressure imposed by the physicochemical conditions during the growth of plant cells in vitro makes it possible to obtain standardized plant material which is available throughout the year unlike plants cultivated in vivo.

Preferably according to the invention, a plant derived from culture in vivo is used.

Any extraction method known to those skilled in the art can be used to prepare the carotenofde used according to the invention.

The carotenoid can be in alcoholic solution, in particular ethanolic. The carotenoid can also be in lipid (oil) or lipoalcoholic solution.

By way of example, according to the invention, a tomato extract rich in lycopene is used, prepared by the company Métaphar, marketed under the name LycOMato® consisting of an oleoresin extract (fatty phase) containing 6% of pure lycopene. .

It is also possible to use, according to the invention, any preparation containing lycopene having the objective of improving the bioavailability of the latter.

The amount of carotenoid that can be used according to the invention is of course a function of the desired effect and can therefore vary to a large extent.

To give an order of magnitude, in the composition according to the invention the carotenoid in the pure state is in an amount representing from 10 ^{"11 to 2} % to 20% of the total weight of the composition and preferably in an amount representing from 10 ^{' 10} % to 10% of the total weight of the composition.

Of course the skilled person, if he uses the carotenoid in the form of a solution, a plant extract for example, knows how to adjust the amount of solution he uses in its composition so that the final amount of carotenoid in the composition is in agreement with the usable quantities previously defined.

Vitamin C (or ascorbic acid) is known to stimulate the synthesis of collagen, by preventing, as a co-factor, the auto-inactivation of the enzymes lysine- and proline-hydroxylases and by increasing the synthesis of the rRNA of procollagènes L ^ascorbic acid (or vitamin C) is also known to stimulate the synthesis of elastin of the skin. We can cite in this respect the patents

US 5801 192. US 4983382 and EP 0717983. We can also cite an article entitled "Pola to încorporate vîtamin C in new cosmetics tîne for skin cars du

Japan Economie Journal of June 5, 1,984 (page 1 5). Thus, it has been described that the ascorbic acid used in cosmetic compositions makes it possible to treat in particular your wrinkles (Fragrance Journal, Vol. 8, n ° 6 (45) (1980) pp3δ- ⁴ 3,

"Cosmetîc and vitamin - action and safety to dermatology"),

According to the invention ascorbic acid and / or its analogs can be used alone or in mixtures of any kind and any proportion and can be of natural or synthetic origin. Ascorbic acid is generally in L form, because it is more particularly in this form that it is found in the plant world.

The amount of vitamin C usable according to the invention is of course a function of the desired effect and can therefore vary to a large extent.

To give an order of magnitude, in the composition of the invention, vitamin C in the pure state, perhaps in an amount representing from 10 ^" 10 10% to 20% of the total weight of the composition and preferably in an amount representing 10 ^{" 8} % to 10% of the total weight of the composition.

Of course, if vitamin C is present in the form of a solution, for example a plant extract, the skilled person will be able to adjust the amount of this solution in the composition according to the invention, so as to obtain the ranges of vitamin C concentrations described above.

The composition of the invention can be in any imaginable form, suitable both for topical application to the skin and / or mucous membranes and / or the hair and / or your hair as for administration by the oral route.

Preferably, the composition of ^" the invention is intended for administration by the oral route.

The composition of the invention can be a cosmetic or dermatological composition. Preferably according to the invention, the composition is a cosmetic composition. The composition is a cosmetic composition because it is intended to improve the general cutaneous appearance of the individual who uses it. Very preferably the composition of the invention is a cosmetic composition intended for administration by the oral route,

For administration by the oral route, the composition of the invention can be in any suitable form, particularly in the form of an oral solution, a syrup, a tablet, a dragee, a capsule or a capsule or a nutritional food or ^a nutritional supplement. Said composition can also comprise at least one suitable excipient suitable for oral administration,

For administration by topical application to the skin, hair, body hair and / or mucous membranes, the composition according to the invention obviously comprises a cosmetically acceptable carrier, that is to say a carrier compatible with the skin, the mucous membranes, the nails, the hair, the hairs and can be in all the galenic forms normally used for a topical application, in particular in the form of an aqueous, hydroalcoholic or oily solution, of an oil-in-water or water-in emulsion oil or multiple, of an aqueous or oily gel, of a liquid, pasty or solid anhydrous product, of an oil dispersion in an aqueous phase using spherules, these spherules possibly being polymeric nanoparticles such than nanospheres and nanocapsules or better lipid vesicles of ionic and / or nonionic type.

This composition can be more or less fluid and have the appearance of a white or colored cream, an ointment, a milk, a lotion, a serum, a paste, a foam. . It can optionally be applied to the skin in the form of an aerosol. It can also be in solid form, and for example in the form of a stick. It can be used as a care product, as a cleaning product, as a makeup product or even as a simple deodorant product,

In a known manner, the composition of the invention may contain the adjuvants customary in the cosmetic and dermatological fields, such as hydrophilic or lipophilic gelling agents. hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, perfumes, fillers, filters, pigments, chelating agents, odor absorbers and coloring matters. The amounts of these various adjuvants are those conventionally used in the fields considered and for example from 0.01% to 20% of the total weight of the composition. These adjuvants, depending on their nature, can be introduced into the fatty phase, into the aqueous phase, into the lipid vesicles and / or into the nanoparticles.

When the composition of the invention is an emulsion, the proportion of the fatty phase can range from 5% to 80% by weight, and preferably from 5% to 50% of the total weight of the composition. Oils, emulsifiers and coemulsifiers used in the composition in the form of an emulsion are chosen from those conventionally used in the field under consideration. The emulsifier and the coemulsifier are present in the composition in a proportion ranging from 0.3% to 30% by weight, and preferably from 0.5% to 20% of the total weight of the composition.

As oils which can be used in the invention, mention may be made of mineral oils, oils of vegetable origin (apricot oil, sunflower oil), oils of animal origin, synthetic oils, silicone oils and oils. fluorinated (perfluoropolyethers). Fatty alcohols (cetyl alcohol), fatty acids, waxes (beeswax) can also be used as fats.

As emulsifiers and coemulsifiers which can be used in the invention, mention may, for example, be made of fatty acid and polyethylene glycol esters such as PEG-40 stearate, PEG-100 stearate, fatty acid and polyol esters such as glyceryl stearate and sorbitan tristearate.

As hydrophilic gelling agents, mention may in particular be made of carboxyvinyl polymers (carbomers). acrylic copolymers such as acrylate / alkylacrylate copolymers, polyacrylamides, polysaccharides, natural gums and clays, and, as lipophilic gelling agents, mention may be made of modified clays such as bentones, metal salts of fatty acids, hydrophobic silica and polyethylenes,

The composition can contain other hydrophilic active agents such as proteins or protein hydrolysates, amino acids, polyols, urea, allantoin, sugars and sugar derivatives, plant extracts and hydroxy acids.

As lipophilic active ingredients. retinol (vitamin A) and its derivatives, to opherol (vitamin E) and its derivatives, essential fatty acids, cerainides, essential oils, salicylic acid and its derivatives or even vitamins B 1 can be used, B6 and / or B12.

It is also possible to use in the composition of the invention at least one isoflavonoid, optionally a plant extract rich in isoflavonoids. such as for example the soy extract (Glycîna max) available from Archer Daniels Midland Company under the name Novasoy ©. A preferred composition of the invention comprises, among other ingredients, the combination of at least one carotenoid and vitamin C, a plant extract rich in isoflavonoids and -tocopherol.

Even more preferably, the composition of the invention comprises, among other ingredients, the combination of β-carotene and vitamin C, soy extract (Novasoy® from the company Archer Daniels Midland Company), and acetate d 'α-tocopherol.

It is also possible to use, in addition, in the composition of the invention, compounds chosen from

- trace elements;

- plant hormones;

- calcium antagonists, such as verapamil and Diltiazem;

- OH radical sensors, such as dimethylstilfoxide;

- chlorine channel openers;

- plant extracts, extracts of micro-organisms including in particular bacterial extracts such as those of non-photosynthetic filamentous bacteria,

To the list above, other compounds can also be added, namely for example your potassium channel openers such as diazoxide and mtnoxidil. spiroxazone, phospholipids such as lecithin, linoleic and linolenic acids, salicylic acid and its derivatives described in French patent FR 2 581 542, such as salicylic acid derivatives carrying an alkanoyl group having from 2 to 12 carbon atoms in position 5 of the benzene ring, hydroxycarboxylic or ketocarboxylic acids and their esters, lactones and their corresponding salts, anthralin, carotenoids, eicosatetraenoic and eicosatrienoic acids or their esters and amides, vitamin D and its derivatives.

According to the invention, can be, among others, added to the composition of the invention ^with other active agents intended in particular for pr évention and / or treatment of skin disorders. Among these active agents, mention may be made, by way of example: - agents modulating differentiation and / or proliferation and / or skin pigmentation such as retinoic acid and its isomers, retinol and its esters, vitamin D and its derivatives, estrogens such as estradlol, kojic acid or hydroquinone; - Antibacterials such as clindamycin phosphate, erythromycin or antibiotics of the tetracycline class;

- agents modulating bacterial adhesion to the skin and / or mucous membranes such as honey, in particular acacia honey and certain sugar derivatives; - antiparasitic agents, in particular metronidazole, crotamion or pyrethroids;

antifungals, in particular compounds belonging to the class of imidazoles such as econazole, ketoconazole or miconcon or their salts, polyene compounds, such as amphotericin B, compounds of the allylamine family, such as terbinafine, or even the octoprox;

- antiviral agents such as acyclovir;

- steroidal anti-inflammatory agents, such as hydrocortisone, betamethasone valerate or clobetasol propionate, or non-steroidal anti-inflammatory agents such as ibuprofen and its salts, diclofenac and its salts, acetylsalicylic acid , acetaminophen or giycyrrhetinic acid;

- anesthetic agents such as lidocaine hydrochloride and its derivatives;

- antipruritic agents such as thenaldine, trimeprazine or cyproheptadine;

- keratolytic agents such as alpha- and beta-hydroxycarboxylic or beta-ketocarboxylic acids, their salts, amides or esters and more particularly hydroxy acids such as glycolic acid, lactic acid, salicylic acid, citric acid and generally fruit acids, and n-octanoyl-5-sa cyclic acid;

- anti-free radical agents, such as superoxide clismutases, certain metal chelators or ascorbic acid and its esters,

- anti-seborrheic drugs such as progesterone,

- anti-dandruff agents such as octopirox or zinc pyrithione;

- anti-acne drugs such as retinic acid or benzoyl peroxide.

- substances such as substance P, CGRP or bradykmine antagonists or MO synthase inhibitors or sodium channel inhibitors, compounds described as being active in the treatment of sensitive skin and as having anti-irritant effects, in particular with regard to irritant compounds possibly present in the compositions.

As active agents, moisturizers such as polyols (for example glycerin), vitamins (for example D-panthenol), anti-inflammatory agents, soothing agents (allantoin, blueberry water), filters can be used UVA and UVB, matting agents (for example polydimethylorganosiloxanes partially crosslinked sold under the name KSG® by Shin Etsu) and their mixtures.

Anti-wrinkle active agents can also be added, and in particular tensing products such as vegetable proteins and their hydrolysates, in particular the soy protein extract sold under the name Eleseryl® by the company LSN or the oat derivative sold. under the name Reductine® by the company Silab.

Since the skin is made up of many other components than collagen and fibroblasts, it turns out to be advantageous, when using the combination of the invention, to promote at the same time the synthesis of these other components such as for example lipids and / or promote the proliferation of other cellular components such as, for example, keratinocytes

Thus, the subject of the invention is a cosmetic composition comprising, in a cosmetically acceptable medium, the combination of at least one carotenoid and vitamin C and at least one other product stimulating the synthesis of lipids and / or the proliferation of keratinocytes. Said composition can be intended for use in the cosmetic or dermatological field, preferably in the cosmetic field.

In this composition, vitamin C and the carotenoid can be as described previously in the text

Mention may be made, as product stimulating the synthesis of lipids, of plant hormones, such as auxins, or of compounds of plant origin, such as kinetic acid, and as product stimulating the proliferation of keratinocytes of compounds of plant origin, such as phloroglucinol.

Thus, the compositions according to the invention can comprise, in addition to the combination of the invention, cinnamic acid or its derivatives and / or a plant hormone, particularly an auxin chosen from indolacetic acid (IAA), 4-chloroindole-3-acetic acid (4-CI-IAA), phenylacetic acid (PAA) and indole-3-butyric acid (IBA). 2,4-dichlorophenoxyacetic acid (2,4-D), r /.- naphthaleneacetic acid (-NAA), β-naphthoxyacetic acid, indole ethanol, Idol acetaldehyde and indole acetonitrile and / or a plant compound such as phloroglucinol. A subject of the invention is also the use of the combination of vitamin C and at least one carotenoid and at least one other product stimulating the synthesis of lipids and / or the proliferation of keratinocytes in a composition or for the preparation of a composition, the combination or the composition being intended to treat, preventively and / or curatively, the cutaneous signs of aging, to combat collagen degradations, to inhibit the expression of proteases of the extracellular matrix , to inhibit the expression of metalloproteinases, particularly metalloproteinase type 1, to treat skin disorders linked to menopause, to fight against fine lines and wrinkles, to fight against withered skin, to fight against soft skin, to fight against thinned skin, to fight against dull and / or dull skin, to fight against the lack of elasticity and / or tone of the skin, to fight against degradations of the skin following exposure to ultraviolet radiation.

In this respect, mention may be made, as product stimulating the synthesis of lipids, of plant hormones, such as auxins, or of compounds of vegetable origin, such as cinnalmic acid, and as product stimulating the proliferation of keratinocytes of compounds of vegetable origin, phloroglucinol.

Thus, your compositions according to the invention may comprise, in addition to the combination of the invention, cinnamic acid or its derivatives and / or a plant hormone, particularly an auxin chosen from indolacetic acid (IAA), l 4-chloroindole-3-acetic acid (4-CI-IAA), phenylacetic acid (PAA), Indole-3-butyric acid (IBA). 2,4-dichlorophenoxyacetic acid (2,4-D), u-naphthalene acetic acid (α-NAA), fi-naphthoxyacetic acid, indole ethanol, idol acetaldehyde and indole acetonitrile and / or a plant compound such as phloroglucinol.

The present invention further relates to a method of cosmetic treatment of the skin intended to stimulate the synthesis of collagen and / or fight against skin disorders linked to age and / or menopause and / or fight against thinning. of the dermis and / or combat the appearance of soft and / or wrinkled skin, characterized in that ('it is applied to the skin, on the hair, on the hair and / or on the mucous membranes or which is ingested a cosmetic composition comprising at least the combination of at least one carotenoid and vitamin C.

The cosmetic treatment process of the invention can be implemented in particular by applying the cosmetic compositions as defined above, according to the usual technique for using these compositions. For example: application of creams, gels, serums, lotions, milks, shampoos or sunscreen compositions, on the skin or on the hair or on the hairs or also application of toothpaste on the gums and so preferable by oral administration of an oral solution, a syrup, a tablet, a capsule, a capsule or a nutrient food or a nutrient supplement.

The following examples and compositions illustrate the invention without limiting it in any way. In the compositions the proportions indicated are percentages by weight.

Composition 1 - Soft capsules: Excipients: Soybean Oil 40 mg

Wheat Germ Oil 85 mg

Soy Lecithins 25 mg

Vitamins; Natural Tocopherols 3 mg

Vitamin C 60 mg

Components:

Lycopene 6 mg Soy isoflavones 50 mg

Composition 2: Care cream (oil in water emulsion, '

Lycopene 6% (LycomatoΘ) 0.001%

Vitamin C 5.00% Glycrol stearate 2.00 '%

Polysorbate 60 (Tween 60 'sold by ICI) 1.00%

Stearic acid 1.40%

Triethanolamine 0.70%

Carbomer 0.40% Fraction, shea butter liquid 12.00%

Perhydrosqualene 12.00%

Antioxidant 0.05%

Perfume 0.50% Preservative 0.30% Water qs 100.00%

Composition 3: Lycopene 6% skin gel (Lycomato®) 0.0001%

Vitamin C 5.00%

Soy isoflavones 0.001%

All trans retinoic acid, 0.05%

Hydroxypropylcellulose (Klucel H ^® sold by the company Hercules) 1.00% Antioxidant 0.05%

Isopropanol 40.00%

Preservative 0.30% Water qs 100.00%

Composition 4: Care cream (oil-in-water emulsion)

Lycopene 6% (Lycomato®) 0.01%

Vitamin C 2.00%

Glycerol stearate 2.00% Polysorbate 60 (Tween 60 ^® sold by the company ICI) 1.00%

Stearic acid 1.40%

Glycyrrhetinic acid 2.00%

Triethanolamine 0.70%

Carbomer 0.40% Liquid fraction of shea butter 12.00%

Sunflower oil 1 0.00%

Antioxidant 0.05%

Perfume 0.50%

Preservative 0.30% Water qs 100.00%

Claims

1. Use of the combination of at least one carotenoid and vitamin C in a composition or for the preparation of a composition, the association or the 5 composition being intended to treat, preventively and / or curatively, the skin signs of aging. 2. Use of the combination of at least one carotenoid and vitamin C in a composition or for the preparation of a composition, the association or the 10 composition being intended to fight against degradations of collagen. 3. Use of the association of at least one carotenoid and of vitamin C in a composition or for the preparation of a composition, the association or the composition being intended to inhibit the expression of the proteases of the matrix 15 extracellular. 4 Use according to claim 3, characterized in that the combination or your composition are intended to inhibit the expression of metaltoproteinases, 5, Use according to any one of claims 3 or 4, characterized in that the combination or the composition are intended to inhibit the expression of metalloproteinase type 1, 6 Use of the combination of at least one carotenoid and vitamin C in 25 a composition or for the preparation of a composition, the combination or the composition being intended for treating skin disorders linked to menopause 7 Use of the association of at least one carotenoid and vitamin C in a composition or for the preparation of a composition the association ci "la 30 composition being intended being intended to fight against fine lines and wrinkles 8 Use of the combination of at least one carotenoid and vitamin C in a composition or for the preparation of a composition, the combination or the composition being intended to combat withered skin. D -3 9. Use of the association of at least one carotenoid and vitamin C in a composition or for the preparation of a composition, the association or the composition being intended to combat soft skin, 10. Use of the combination of at least one carotenoid and of vitamin C in a composition or for the preparation of a composition, the association or the composition being intended to fight against thinned skin, 1 1, Use of the combination of at least one carotenoid and vitamin C in a composition or for the preparation of a composition, the combination or the composition being intended to combat dull and flaky skin. 12. Use of the combination of at least one carotenoid and vitamin C in a composition or for the preparation of a composition, the association or the composition being intended to combat the lack of elasticity and / or tone skin. 1 3. Use of the combination of at least one carotenoid and of vitamin C in a composition or for the preparation of a composition, the association or the composition being intended to combat internal degradations of the skin consecutive to a exposure to ultraviolet radiation. 14. Use according to any one of the preceding claims, characterized in that the carotenoid is a carotenoid with provitamin A activity or a carotenoid without provitamin A activity or a mixture of the two. 15, Use according to any one of the preceding claims, characterized in that the carotenoid with provitamin activity A is chosen from β-carotene or u-carotene or a mixture of the two. 16. Use according to claim 15, characterized in that the carotenoid with provitamin activity A is B-carotene. 17. Use according to any one of the preceding claims, characterized in that the carotenoid without provitamin activity A is chosen from zeaxanthin, cryptoxanthine, lutein or lycopene or a mixture of these. 1 8 Use according to claim 17, characterized in that the carotenoid without provitamin activity A is lycopene. 19. Use according to any one of the preceding claims, characterized in that the carotenoid in the pure state is in an amount representing from 10 ^"12 % to 20% of the total weight of the composition. 5 20. Use according to any one of the preceding claims, characterized in that the carotenoid in the pure state is in an amount of 1 0 ^'10% o to 10% of the total weight of the composition. I 0 21. Use according to any one of the preceding claims, characterized in that the vitamin C in the pure state is in an amount representing from 1 0 ^"10 % to 20% of the total weight of the composition. 22. Use according to any one of the preceding claims, characterized in that the vitamin C in the pure state is in an amount representing from 10 ^"8 to 10% of the total weight of the composition. 23. Use according to any one of the preceding claims, characterized in that the composition is intended for administration by 20 orally. 24. Use according to any one of the preceding claims, characterized in that the composition is in the form of an oral solution, a syrup, a tablet, a dragee, a capsule, a 25 capsule or a nutritional food or a nutritional supplement. 25. Cosmetic composition comprising at least the combination of at least one carotenoid and vitamin C and at least one other product stimulating the synthesis of lipids and / or the proliferation of keratinocytes 30 26. Composition according to Claim 25, characterized in that the carotenoid is as described in '' any one of Claims 14 to 20 27. Composition according to any one of claims 31 to 33 characterized in that the vitamin C is as described in any one of claims 21 or 22. 28. Composition according to any one of claims 25 to 27, characterized by the fact that the product stimulating the synthesis of lipids is chosen from plant hormones, such as auxins, or -composites of plant origin, such as cinnamic acid or its derivatives. 29. Composition according to any one of claims 25 to 28, characterized in that the auxin is chosen from indolacetic acid (IAA), 4-chloroindole-3-acetic acid (4-CI-IAA) , phenylacetic acid (PAA). indole-3-butyric acid (IBA), 2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaienacetic acid (α-NAA), β-naphthoxyacetic acid, indole ethanol, the acaidahyde idol and the acetonitrile indole 30. Composition according to any one of claims 25 to 29, characterized in that the product stimulating the proliferation of keratinocytes is phloroglucinol. 31. Use of the combination of at least one carotenoid, vitamin C and at least one other product stimulating the synthesis of lipids and / or the proliferation of keratinocytes in a composition or for the preparation of a composition, the combination or the composition being intended to treat, preventively and / or curatively, the cutaneous signs of aging, to combat collagen degradation, to inhibit the expression of proteases of the extracellular matrix, to inhibit the expression of metalloproteinases, particularly metalloproteinase type 1, to treat skin disorders related to menopause, to fight against fine lines and wrinkles, to fight withered skin, to fight against soft skin, to fight against thinned skin, to fight against dull and / or dull skin, to fight against the lack of elasticity and / or tone of the skin, to fight against consecutive internal degradations of the skin exposure to ultraviolet radiation. 32. Method of cosmetic treatment of the skin intended to combat skin disorders linked to age and / or menopause and / or to combat thinning of the dermis and / or to combat the appearance of soft and wrinkled skin , characterized in that it is applied to the skin, the hair, the hair and / or the mucous membranes, or that a cosmetic composition is ingested comprising the combination of at least one carotenoid and vitamin VS 31. Cosmetic treatment method for the skin intended to fight against age-related and / or menopausal skin damage and / or to fight against thinning the dermis and / or combating the appearance of soft, wrinkled skin, characterized in that it is applied to the skin, to the hair, to the hair and / or to the mucous membranes, or a cosmetic composition is ingested a cosmetic composition as described in any one of claims 25 to 30.