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WO2002033104A3 - Antibiotic-based gene regulation system - Google Patents

Antibiotic-based gene regulation system Download PDF

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Publication number
WO2002033104A3
WO2002033104A3 PCT/IB2001/001963 IB0101963W WO0233104A3 WO 2002033104 A3 WO2002033104 A3 WO 2002033104A3 IB 0101963 W IB0101963 W IB 0101963W WO 0233104 A3 WO0233104 A3 WO 0233104A3
Authority
WO
WIPO (PCT)
Prior art keywords
antibiotic
gene regulation
polypeptides
regulation system
based gene
Prior art date
Application number
PCT/IB2001/001963
Other languages
French (fr)
Other versions
WO2002033104A2 (en
Inventor
Martin Fussenegger
James E Bailey
Original Assignee
Cistronics Cell Technology Gmb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cistronics Cell Technology Gmb filed Critical Cistronics Cell Technology Gmb
Priority to AU2002210802A priority Critical patent/AU2002210802A1/en
Publication of WO2002033104A2 publication Critical patent/WO2002033104A2/en
Publication of WO2002033104A3 publication Critical patent/WO2002033104A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a novel system for gene regulation in eukaryotic cells, and methods of using the same for protein production, tissue engineering and gene therapy. In particular, the invention provides a new system for antibiotic-regulated gene expression in eukaryotic cells based on sequences from Actinomycetes antibiotic resistance promoters, polypeptides that bind to the same in an antibiotic responsive manner, and nucleotides encoding such polypeptides. Further, the invention provides novel and sensitive methods of screening for candidate antibiotics.
PCT/IB2001/001963 2000-10-20 2001-10-19 Antibiotic-based gene regulation system WO2002033104A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002210802A AU2002210802A1 (en) 2000-10-20 2001-10-19 Antibiotic-based gene regulation system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69362400A 2000-10-20 2000-10-20
US09/693,624 2000-10-20

Publications (2)

Publication Number Publication Date
WO2002033104A2 WO2002033104A2 (en) 2002-04-25
WO2002033104A3 true WO2002033104A3 (en) 2003-10-02

Family

ID=24785431

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2001/001963 WO2002033104A2 (en) 2000-10-20 2001-10-19 Antibiotic-based gene regulation system

Country Status (2)

Country Link
AU (1) AU2002210802A1 (en)
WO (1) WO2002033104A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017134137A1 (en) * 2016-02-05 2017-08-10 Polygene Ag Glucocorticoid-based gene regulation system

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000065080A1 (en) * 1999-04-23 2000-11-02 Fussenegger, Martin Antibiotic-based gene regulation system

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000065080A1 (en) * 1999-04-23 2000-11-02 Fussenegger, Martin Antibiotic-based gene regulation system

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AUGUST P R ET AL: "BIOSYNTHESIS OF THE ANSAMYCIN ANTIBIOTIC RIFAMYCIN: DEDUCTIONS FROM THE MOLECULAR ANALYSIS OF THE RIF BIOSYNTHETIC GENE CLUSTER OF AMYCOLATOPSIS MEDITERRANEI S699", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 5, no. 2, 13 February 1998 (1998-02-13), pages 69 - 79, XP001084972, ISSN: 1074-5521 *
BLANC V ET AL: "Molecular characterization and transcriptional analysis of a multdrug resistance gene cloned from the pristinamycin-producing organism, Streptomyces pristinaespiralis", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 17, no. 5, 1995, pages 989 - 999, XP008009341, ISSN: 0950-382X *
FOLCHER MARC ET AL: "A transcriptional regulator of a pristinamycin resistance gene in Streptomyces coelicolor.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 2, 12 January 2001 (2001-01-12), pages 1479 - 1485, XP002184465, ISSN: 0021-9258 *
KIM C-G ET AL: "3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 273, no. 11, 13 March 1998 (1998-03-13), pages 6030 - 6040, XP002145258, ISSN: 0021-9258 *
SALAH-BEY K ET AL: "STRESS-ACTIVATED EXPRESSION OF A STREPTOMYCES PRISTINAESPIRALIS MULTIDRUG RESISTANCE GENE (PTR) IN VARIOUS STREPTOMYCES SPP. AND ESCHERICHIA COLI", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 17, no. 5, 1995, pages 1001 - 1012, XP008009342, ISSN: 0950-382X *
SALAH-BEY K ET AL: "UNUSUAL REGULATORY MECHANISM FOR A STREPTOMYCES MULTIDRUG RESISTANCE GENE, PTR, INVOLVING THREE HOMOLOGOUS PROTEIN-BINDING SITES OVERLAPPING THE PROMOTER REGION", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 17, no. 6, 1995, pages 1109 - 1119, XP008009340, ISSN: 0950-382X *

Also Published As

Publication number Publication date
AU2002210802A1 (en) 2002-04-29
WO2002033104A2 (en) 2002-04-25

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