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WO2002028175A3 - Transgenic mouse for targeted recombination mediated by modified cre-er - Google Patents

Transgenic mouse for targeted recombination mediated by modified cre-er Download PDF

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Publication number
WO2002028175A3
WO2002028175A3 PCT/IB2001/002246 IB0102246W WO0228175A3 WO 2002028175 A3 WO2002028175 A3 WO 2002028175A3 IB 0102246 W IB0102246 W IB 0102246W WO 0228175 A3 WO0228175 A3 WO 0228175A3
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Prior art keywords
organism
interest
recombinase
transgenic mouse
targeted recombination
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PCT/IB2001/002246
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French (fr)
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WO2002028175A9 (en
WO2002028175A2 (en
Inventor
Pierre Chambon
Daniel Metzger
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Ass Pour Le Dev De La Rech
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Publication date
Priority claimed from FR0012570A external-priority patent/FR2814642B1/en
Application filed by Ass Pour Le Dev De La Rech filed Critical Ass Pour Le Dev De La Rech
Priority to EP01986247A priority Critical patent/EP1322152A2/en
Publication of WO2002028175A2 publication Critical patent/WO2002028175A2/en
Publication of WO2002028175A3 publication Critical patent/WO2002028175A3/en
Publication of WO2002028175A9 publication Critical patent/WO2002028175A9/en

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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Abstract

The present invention relates to a metazoan organism, with the exception of humans, and in particular a mouse, characterized in that at least one cell of this organism comprises at least one fusion protein between a recombinase Cre and a modified ligand binding domain of the nuclear estrogen receptor alpha, allowing the inactive fused recombinase to be induced by synthetic antiestrogens, but not by natural estrogens, and one or more DNA sequences of interest belonging to the genome of said organism into which one or more sites of recognition of said recombinase protein are inserted. The invention also covers the methods using said organism for the screening of medicaments, the mutagenesis and the analysis of the biological function of the DNA sequence(s) of interest, in particular of gene(s) of interest, such as RXRα.
PCT/IB2001/002246 2000-10-03 2001-09-28 Transgenic mouse for targeted recombination mediated by modified cre-er WO2002028175A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP01986247A EP1322152A2 (en) 2000-10-03 2001-09-28 Transgenic mouse for targeted recombination mediated by modified cre-er

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR0012570A FR2814642B1 (en) 2000-10-03 2000-10-03 TRANSGENIC MOUSE FOR THE TARGETED RECOMBINATION MEDIATED BY THE MODIFIED CRE-ER
FR00/12570 2000-10-03
US09/853,033 2001-05-11
US09/853,033 US7112715B2 (en) 2000-10-03 2001-05-11 Transgenic mouse for targeted recombination mediated by modified Cre-ER

Publications (3)

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WO2002028175A2 WO2002028175A2 (en) 2002-04-11
WO2002028175A3 true WO2002028175A3 (en) 2003-01-09
WO2002028175A9 WO2002028175A9 (en) 2003-10-30

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Families Citing this family (7)

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Publication number Priority date Publication date Assignee Title
JP2007535906A (en) * 2003-10-24 2007-12-13 イステイチユート・デイ・リチエルケ・デイ・ビオロジア・モレコラーレ・ピ・アンジエレツテイ・エツセ・ピー・アー Orthogonal gene switch
CN100387722C (en) * 2004-02-18 2008-05-14 中国人民解放军军事医学科学院生物工程研究所 Method for preparing transgenic mouse with central nervous system specific expression Cre recombinase
JP2008517600A (en) * 2004-10-22 2008-05-29 セラピューティック ヒューマン ポリクローナルズ， インコーポレイテッド Suppression of endogenous immunoglobulin expression in non-human transgenic animals
EP2067858A1 (en) 2007-12-07 2009-06-10 Universidad de Sevilla Animal models for neurodegenerative diseases
ES2337973B8 (en) 2008-05-16 2011-07-21 Proyecto De Biomedicina Cima, S.L. ADENOVIRUS AUXILIARY SELF-INACTIVANTS FOR THE PRODUCTION OF ADENOVIRUS RECOMBINANTS OF HIGH CAPACITY.
WO2018129203A2 (en) * 2017-01-06 2018-07-12 The Regents Of The University Of California Method for temporal and tissue-specific drug delivery and induced nucleic acid recombination
CN112921052B (en) * 2019-12-06 2023-07-21 中国科学院分子细胞科学卓越创新中心 In vivo cell proliferation labeling and tracing system and its application

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