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WO2002012474A3 - Procede pour la selection concernant des cellules hotes a sequences d'adn eliminees - Google Patents

Procede pour la selection concernant des cellules hotes a sequences d'adn eliminees Download PDF

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Publication number
WO2002012474A3
WO2002012474A3 PCT/DE2001/002511 DE0102511W WO0212474A3 WO 2002012474 A3 WO2002012474 A3 WO 2002012474A3 DE 0102511 W DE0102511 W DE 0102511W WO 0212474 A3 WO0212474 A3 WO 0212474A3
Authority
WO
WIPO (PCT)
Prior art keywords
selection
host cells
dna sequences
eliminated
eliminated dna
Prior art date
Application number
PCT/DE2001/002511
Other languages
German (de)
English (en)
Other versions
WO2002012474A2 (fr
Inventor
Lorenz Buelow
Klaus Duering
Original Assignee
Mpb Cologne Gmbh
Lorenz Buelow
Klaus Duering
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mpb Cologne Gmbh, Lorenz Buelow, Klaus Duering filed Critical Mpb Cologne Gmbh
Priority to AU2001279561A priority Critical patent/AU2001279561A1/en
Publication of WO2002012474A2 publication Critical patent/WO2002012474A2/fr
Publication of WO2002012474A3 publication Critical patent/WO2002012474A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PCT/DE2001/002511 2000-08-03 2001-07-04 Procede pour la selection concernant des cellules hotes a sequences d'adn eliminees WO2002012474A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001279561A AU2001279561A1 (en) 2000-08-03 2001-07-04 Method of selection in host cells with eliminated dna sequences

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2000138573 DE10038573A1 (de) 2000-08-03 2000-08-03 Verfahren zur Selektion auf Wirtszellen mit eliminierten DNA-Sequenzen
DE10038573.7 2000-08-03

Publications (2)

Publication Number Publication Date
WO2002012474A2 WO2002012474A2 (fr) 2002-02-14
WO2002012474A3 true WO2002012474A3 (fr) 2002-04-25

Family

ID=7651654

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/002511 WO2002012474A2 (fr) 2000-08-03 2001-07-04 Procede pour la selection concernant des cellules hotes a sequences d'adn eliminees

Country Status (3)

Country Link
AU (1) AU2001279561A1 (fr)
DE (1) DE10038573A1 (fr)
WO (1) WO2002012474A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12297422B2 (en) 2022-08-26 2025-05-13 Syngulon Sa Controlled growth of microorganisms

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7183097B1 (en) 1998-05-07 2007-02-27 Universite Libre De Bruxelles Cytotoxin-based biological containment
AU2002235676B2 (en) 2001-02-23 2006-10-26 Universite Libre De Bruxelles Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to a toxic molecule
AU2003213889B2 (en) * 2002-03-19 2008-12-04 Universite Libre De Bruxelles Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms
US9309518B2 (en) 2002-09-03 2016-04-12 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
JP4546828B2 (ja) 2002-09-03 2010-09-22 ユニヴェルシテ リブル ドゥ ブリュッセル 可逆的で、平行的で、多数の仕事を行うことができるクローニング方法及びキット
DE10256042B4 (de) * 2002-11-30 2006-06-08 Universität Potsdam Verfahren zur Selektion von Zellen, die spezifisch bindende Moleküle produzieren
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
WO2009150441A1 (fr) * 2008-06-13 2009-12-17 University Of Stavanger Transformation mitochondriale
ES2893454T3 (es) 2013-08-19 2022-02-09 Syngulon Sa Crecimiento controlado de microorganismo
BR112020012543A2 (pt) 2017-12-19 2021-02-23 Syngulon S.A. processo de fermentação

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995000555A1 (fr) * 1993-06-28 1995-01-05 European Molecular Biology Laboratory (Embl) Regulation de la recombinaison a des sites specifiques par des proteines resultant de la fusion d'une recombinase a des sites specifiques et d'un recepteur nucleaire
WO1996004393A2 (fr) * 1994-08-01 1996-02-15 Delta And Pine Land Company Regulation de l'expression d'un gene de plante
WO1997013401A1 (fr) * 1995-10-13 1997-04-17 Purdue Research Foundation Procede de production de plantes hybrides
WO1997022707A1 (fr) * 1995-12-19 1997-06-26 Cerff Ruediger Systeme d'expression pour l'expression genique anaerobie dans des plantes superieures
WO1997041228A2 (fr) * 1996-05-01 1997-11-06 Pioneer Hi-Bred International, Inc. Utilisation de la proteine fluorescente verte comme marqueur de criblage pour la transformation de plantes
WO2001021768A1 (fr) * 1999-09-21 2001-03-29 Rutgers, The State University Of New Jersey Systeme de recombinaison specifique au site permettant de manipuler le genome du plaste de plantes superieures
WO2001088164A1 (fr) * 2000-05-19 2001-11-22 Mpb Cologne Gmbh Elimination inductible de sequences d'adn dans des plantes

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
DE19834430C2 (de) * 1998-07-30 2000-05-31 Harald Von Melchner Selbstdeletierende Vektoren für die Krebstherapie

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995000555A1 (fr) * 1993-06-28 1995-01-05 European Molecular Biology Laboratory (Embl) Regulation de la recombinaison a des sites specifiques par des proteines resultant de la fusion d'une recombinase a des sites specifiques et d'un recepteur nucleaire
WO1996004393A2 (fr) * 1994-08-01 1996-02-15 Delta And Pine Land Company Regulation de l'expression d'un gene de plante
WO1997013401A1 (fr) * 1995-10-13 1997-04-17 Purdue Research Foundation Procede de production de plantes hybrides
WO1997022707A1 (fr) * 1995-12-19 1997-06-26 Cerff Ruediger Systeme d'expression pour l'expression genique anaerobie dans des plantes superieures
WO1997041228A2 (fr) * 1996-05-01 1997-11-06 Pioneer Hi-Bred International, Inc. Utilisation de la proteine fluorescente verte comme marqueur de criblage pour la transformation de plantes
WO2001021768A1 (fr) * 1999-09-21 2001-03-29 Rutgers, The State University Of New Jersey Systeme de recombinaison specifique au site permettant de manipuler le genome du plaste de plantes superieures
WO2001088164A1 (fr) * 2000-05-19 2001-11-22 Mpb Cologne Gmbh Elimination inductible de sequences d'adn dans des plantes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANGRAND P-O ET AL: "INDUCIBLE EXPRESSION BASED ON REGULATED RECOMBINATION: A SINGLE VECTOR STRATEGY FOR STABLE EXPRESSION IN CULTURED CELLS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 13, 1998, pages 3263 - 3269, XP001021788, ISSN: 0305-1048 *
DALE E C ET AL: "GENE TRANSFER WITH SUBSEQUENT REMOVAL OF THE SELECTION GENE FROM THE HOST GENOME", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 88, 1 December 1991 (1991-12-01), pages 10558 - 10562, XP002043803, ISSN: 0027-8424 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12297422B2 (en) 2022-08-26 2025-05-13 Syngulon Sa Controlled growth of microorganisms

Also Published As

Publication number Publication date
WO2002012474A2 (fr) 2002-02-14
AU2001279561A1 (en) 2002-02-18
DE10038573A1 (de) 2002-02-21

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