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WO2002010751A3 - Microdissection de transfert - Google Patents

Microdissection de transfert Download PDF

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Publication number
WO2002010751A3
WO2002010751A3 PCT/US2001/008095 US0108095W WO0210751A3 WO 2002010751 A3 WO2002010751 A3 WO 2002010751A3 US 0108095 W US0108095 W US 0108095W WO 0210751 A3 WO0210751 A3 WO 0210751A3
Authority
WO
WIPO (PCT)
Prior art keywords
biomolecules
transfer layer
transfer
interest
altered
Prior art date
Application number
PCT/US2001/008095
Other languages
English (en)
Other versions
WO2002010751A8 (fr
WO2002010751A2 (fr
Inventor
Michael R Emmert-Buck
Chad R Haldeman-Englert
Robert F Bonner
Lance A Liotta
Original Assignee
Government Of The Us Sec Dep O
Michael R Emmert-Buck
Chad R Haldeman-Englert
Robert F Bonner
Lance A Liotta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Government Of The Us Sec Dep O, Michael R Emmert-Buck, Chad R Haldeman-Englert, Robert F Bonner, Lance A Liotta filed Critical Government Of The Us Sec Dep O
Priority to US10/333,374 priority Critical patent/US20040053326A1/en
Priority to EP01918647A priority patent/EP1305622A2/fr
Priority to AU2001245700A priority patent/AU2001245700A1/en
Priority to CA002415864A priority patent/CA2415864A1/fr
Publication of WO2002010751A2 publication Critical patent/WO2002010751A2/fr
Publication of WO2002010751A3 publication Critical patent/WO2002010751A3/fr
Publication of WO2002010751A8 publication Critical patent/WO2002010751A8/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
    • G01N2001/284—Collecting samples on a sticky, tacky, adhesive surface using local activation of adhesive, i.e. Laser Capture Microdissection

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne des procédés, des systèmes et des dispositifs permettant d'analyser un matériau biologique, tel qu'un spécimen cellulaire ou autre. Selon un mode de réalisation, la méthode consiste à transférer sélectivement et focalement des biomolécules d'une région cible d'intérêt à un échantillon biologique (tel qu'un fragment de tissu). Pour le transfert, on peut, par exemple, modifier sélectivement une couche de transfert adjacente à la région cible de sorte que la biomolécule puisse traverser la zone modifiée de la couche de transfert. Dans des exemples particuliers, on modifie la couche de transfert en en augmentant focalement la perméabilité, par exemple en retirant une partie focale de la couche de transfert et en transportant des biomolécules au travers de la région modifiée de manière à pouvoir séparer par microdissection des molécules d'intérêt de l'échantillon biologique. Selon d'autres modes de réalisation, les biomolécules soumises à microdissection peuvent être appliquées sur un substrat d'analyse renfermant une molécule d'identification, tel qu'un jeu d'acides nucléiques, un champ de balayage d'expression en couche, ou des puits renfermant des anticorps. La microdissection de transfert permet d'analyser sélectivement des biomolécules extraites de régions d'intérêt. Il est par exemple possible de déterminer l'expression différentielle de certaines protéines par l'analyse de nids de cellules fortement atypiques ou métastatiques dans une partie de tumeur.
PCT/US2001/008095 2000-07-26 2001-03-14 Microdissection de transfert WO2002010751A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US10/333,374 US20040053326A1 (en) 2001-03-14 2001-03-14 Transfer microdessection
EP01918647A EP1305622A2 (fr) 2000-07-26 2001-03-14 Microdissection de transfert
AU2001245700A AU2001245700A1 (en) 2000-07-26 2001-03-14 Transfer microdissection
CA002415864A CA2415864A1 (fr) 2000-07-26 2001-03-14 Microdissection de transfert

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22090400P 2000-07-26 2000-07-26
US60/220,904 2000-07-26

Publications (3)

Publication Number Publication Date
WO2002010751A2 WO2002010751A2 (fr) 2002-02-07
WO2002010751A3 true WO2002010751A3 (fr) 2002-04-25
WO2002010751A8 WO2002010751A8 (fr) 2004-03-04

Family

ID=22825494

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/008095 WO2002010751A2 (fr) 2000-07-26 2001-03-14 Microdissection de transfert

Country Status (4)

Country Link
EP (1) EP1305622A2 (fr)
AU (1) AU2001245700A1 (fr)
CA (1) CA2415864A1 (fr)
WO (1) WO2002010751A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1521961A2 (fr) * 2002-07-05 2005-04-13 The University of Chicago Caracterisation de biopuces contenant des monocouches auto-assemblees
US20040077073A1 (en) * 2002-10-18 2004-04-22 Melvin Schindler Methods and apparatus for interactive micromanipulation of biological materials
US7695752B2 (en) 2003-01-24 2010-04-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target activated microtransfer
US7709047B2 (en) 2003-01-24 2010-05-04 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target activated microtransfer
DE10358565B4 (de) * 2003-12-15 2007-06-28 P.A.L.M. Microlaser Technologies Ag Aufnahmeelement zum Aufnehmen eines aus einer biologischen Masse mittels Laserstrahlung herausgelösten Objekts und Verfahren zur Gewinnung und Verarbeitung eines biologischen Objekts
DE102004041941B4 (de) * 2004-08-30 2007-01-11 P.A.L.M. Microlaser Technologies Ag Verfahren zur Gewinnung von biologischen Objekten mit einer Aufnahmeeinheit
FR2883070B1 (fr) * 2005-03-08 2007-05-18 Inst Nat Sante Rech Med Procede et systeme de determination in vitro de la reactivite d'une entite d'interet sur un element fonctionnel tridimensionnel
DE102005061561A1 (de) * 2005-12-22 2007-06-28 P.A.L.M. Microlaser Technologies Ag Laser-Mikrodissektionsverfahren, Steuersystem für eine Laser-Mikrodissektionsvorrichtung und Trägervorrichtung
DE102006000934A1 (de) * 2006-01-05 2007-07-12 P.A.L.M. Microlaser Technologies Ag Mikrodissektionsverfahren und Mikrodissektionssystem
DE102013212811A1 (de) * 2013-07-01 2015-01-08 Leica Microsystems Cms Gmbh Lasermikrodissektionssystem und Untersuchungsverfahren für nukleinsäurehaltige Proben
CA3058904A1 (fr) * 2017-04-07 2018-10-11 Michael R. Emmert-Buck Systeme de micropurification de precision elevee et procedes d'utilisation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997013838A1 (fr) * 1995-10-10 1997-04-17 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Isolation d'un materiau sous visualisation microscopique
WO2001007915A2 (fr) * 1999-07-26 2001-02-01 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Methode et dispositif d'analyse de specimens biologiques

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997013838A1 (fr) * 1995-10-10 1997-04-17 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Isolation d'un materiau sous visualisation microscopique
WO2001007915A2 (fr) * 1999-07-26 2001-02-01 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Methode et dispositif d'analyse de specimens biologiques

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BANKS R E ET AL: "THE POTENTIAL USE OF LASER CAPTURE MICRODISSECTION TO SELECTIVELY OBTAIN DISTINCT POPULATIONS OF CELLS FOR PROTEOMIC ANALYSIS - PRELIMINARY FINDINGS", ELECTROPHORESIS, WEINHEIM, DE, vol. 20, no. 4/5, April 1999 (1999-04-01), pages 689 - 700, XP000925546, ISSN: 0173-0835 *
EMMERT-BUCK M R ET AL: "LASER CAPTURE MICRODISSECTION", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 274, no. 5289, 8 November 1996 (1996-11-08), pages 998 - 1001, XP000644727, ISSN: 0036-8075 *
ENGLERT CHAD R ET AL: "Layered expression scanning: Rapid molecular profiling of tumor samples", CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, BALTIMORE, MD, US, vol. 60, no. 6, 15 March 2000 (2000-03-15), pages 1526 - 1530, XP002156603, ISSN: 0008-5472 *

Also Published As

Publication number Publication date
CA2415864A1 (fr) 2002-02-07
WO2002010751A8 (fr) 2004-03-04
EP1305622A2 (fr) 2003-05-02
AU2001245700A1 (en) 2002-02-13
WO2002010751A2 (fr) 2002-02-07

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