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WO2002004613A3 - Isolated polynucleotides encoding human 5'-nucleotide cn-ia and cn-ib, isolated proteins encoded by the same, and methods utilizing the same - Google Patents

Isolated polynucleotides encoding human 5'-nucleotide cn-ia and cn-ib, isolated proteins encoded by the same, and methods utilizing the same Download PDF

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Publication number
WO2002004613A3
WO2002004613A3 PCT/US2001/021406 US0121406W WO0204613A3 WO 2002004613 A3 WO2002004613 A3 WO 2002004613A3 US 0121406 W US0121406 W US 0121406W WO 0204613 A3 WO0204613 A3 WO 0204613A3
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WO
WIPO (PCT)
Prior art keywords
same
human
isolated
encodes
seq
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Application number
PCT/US2001/021406
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French (fr)
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WO2002004613A2 (en
Inventor
Jozef Spychala
Beverly S Mitchell
Sally Hunsucker
Yanshan Ji
Original Assignee
Univ North Carolina
Jozef Spychala
Beverly S Mitchell
Sally Hunsucker
Yanshan Ji
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Publication date
Application filed by Univ North Carolina, Jozef Spychala, Beverly S Mitchell, Sally Hunsucker, Yanshan Ji filed Critical Univ North Carolina
Priority to AU2001273224A priority Critical patent/AU2001273224A1/en
Publication of WO2002004613A2 publication Critical patent/WO2002004613A2/en
Publication of WO2002004613A3 publication Critical patent/WO2002004613A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The isolated polynucleotide sequence identified herein as SEQ ID NO: 1 encodes an human cytosolic 5'-nucleotidase form I (human cN-IA) and encodes the protein human cN-IA of SEQ ID NO:2. The isolated polynucleotide sequence identified herein as SEQ ID NO: 3 encodes a cytosolic 5'-nucleotidase form I (cN-IB) and encodes the protein cN-IB of SEQ ID NO:4. Expression of human cN-IA decreases the efficacy of antineoplastic drugs and increases the toxicity of AZT. Inhibitors of human cN-IA are useful in methods of treating cancer by increasing the efficacy of antineoplastic drugs, and in methods of treating viral infections such as HIV infection and AIDS by decreasing the side effects of AZT.
PCT/US2001/021406 2000-07-07 2001-07-06 Isolated polynucleotides encoding human 5'-nucleotide cn-ia and cn-ib, isolated proteins encoded by the same, and methods utilizing the same WO2002004613A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001273224A AU2001273224A1 (en) 2000-07-07 2001-07-06 Isolated polynucleotides encoding human 5'-nucleotide cn-ia and cn-ib, isolated proteins encoded by the same, and methods utilizing the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21682700P 2000-07-07 2000-07-07
US60/216,827 2000-07-07

Publications (2)

Publication Number Publication Date
WO2002004613A2 WO2002004613A2 (en) 2002-01-17
WO2002004613A3 true WO2002004613A3 (en) 2003-02-13

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PCT/US2001/021406 WO2002004613A2 (en) 2000-07-07 2001-07-06 Isolated polynucleotides encoding human 5'-nucleotide cn-ia and cn-ib, isolated proteins encoded by the same, and methods utilizing the same

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AU (1) AU2001273224A1 (en)
WO (1) WO2002004613A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012154933A1 (en) * 2011-05-10 2012-11-15 The Brigham And Women's Hospital, Inc. Detecting inclusion body myositis
PL2729810T3 (en) * 2011-07-07 2019-03-29 Stichting Katholieke Universiteit Myositis
DK3221346T3 (en) 2014-11-21 2020-10-12 Bristol Myers Squibb Co ANTIBODIES INCLUDING MODIFIED CONSTANT AREAS OF THE HEAVY CHAIN
HUE050596T2 (en) 2014-11-21 2020-12-28 Bristol Myers Squibb Co Antibodies against cd73 and uses thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989009603A1 (en) * 1988-04-12 1989-10-19 Brown University Research Foundation Treatment of viral infections
WO1996033203A1 (en) * 1995-04-21 1996-10-24 University Of South Florida Immunopotentiating inosine monophosphate 5'-nucleotidase resistant derivatives and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989009603A1 (en) * 1988-04-12 1989-10-19 Brown University Research Foundation Treatment of viral infections
WO1996033203A1 (en) * 1995-04-21 1996-10-24 University Of South Florida Immunopotentiating inosine monophosphate 5'-nucleotidase resistant derivatives and uses thereof

Non-Patent Citations (21)

* Cited by examiner, † Cited by third party
Title
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BHALLA K ET AL: "2' DEOXYCYTIDINE PROTECTS NORMAL HUMAN BONE MARROW PROGENITOR CELLS IN-VITRO AGAINST THE CYTOTOXICITY OF 3' AZIDO-3'-DEOXYTHYMIDINE WITH PRESERVATION OF ANTIRETROVIRAL ACTIVITY", BLOOD, vol. 74, no. 6, 1989, pages 1923 - 1928, XP008005334, ISSN: 0006-4971 *
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GARVEY EDWARD P ET AL: "A specific inhibitor of heart cytosolic 5'-nucleotidase I attenuates hydrolysis of adenosine 5'-monophosphate in primary rat myocytes.", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 364, no. 2, 15 April 1999 (1999-04-15), pages 235 - 240, XP002204514, ISSN: 0003-9861 *
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MOHAMMAD RAMZI M ET AL: "Potentiation of 2-chlorodeoxyadenosine activity by bryostatin 1 in the resistant chronic lymphocytic leukemia cell line (WSU-CLL): Association with increased ratios of dCK/5'-NT and Bax/Bcl-2.", BIOLOGICAL CHEMISTRY, vol. 379, no. 10, October 1998 (1998-10-01), pages 1253 - 1261, XP008005331, ISSN: 1431-6730 *
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RAMILO-TORNO LILIBETH V ET AL: "Intracellular pharmacodynamic studies of the synergistic combination of 6-mercaptopurine and cytosine arabinoside in human leukemia cell lines.", CANCER CHEMOTHERAPY AND PHARMACOLOGY, vol. 35, no. 3, 1995, pages 191 - 199, XP008005333, ISSN: 0344-5704 *
SALA-NEWBY G.B.; ET AL: "The mechanism of adenosine formation in cells. Cloning of cytosolic 5'-nucleotidase-I", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, 1999, pages 17789 - 17793, XP002203597 *
SALA-NEWBY GRACIELA B ET AL: "Distinct roles for recombinant cytosolic 5'-nucleotidase-I and -II in AMP and IMP catabolism in COS-7 and H9c2 rat myoblast cell lines.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 16, 21 April 2000 (2000-04-21), pages 11666 - 11671, XP002203573, ISSN: 0021-9258 *
SOMMADOSSI J P: "Update on the cellular and molecular pharmacology of nucleosides.", PATHOLOGIE BIOLOGIE, vol. 41, no. 8 SUPPL., 1993, pages 781 - 787, XP008005133, ISSN: 0369-8114 *
SPYCHALA JOZEF ET AL: "ATP and phosphate reciprocally affect subunit association of human high Km 5'-nucleotidase: Role for the C-terminal polyglutamic acid tract in subunit association and catalytic activity.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 259, no. 3, February 1999 (1999-02-01), pages 851 - 858, XP002203572, ISSN: 0014-2956 *

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AU2001273224A1 (en) 2002-01-21

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