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WO2002068684A3 - Méthode d'extension d'amorce spécifique d'allèle - Google Patents

Méthode d'extension d'amorce spécifique d'allèle Download PDF

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Publication number
WO2002068684A3
WO2002068684A3 PCT/GB2002/000794 GB0200794W WO02068684A3 WO 2002068684 A3 WO2002068684 A3 WO 2002068684A3 GB 0200794 W GB0200794 W GB 0200794W WO 02068684 A3 WO02068684 A3 WO 02068684A3
Authority
WO
WIPO (PCT)
Prior art keywords
primer extension
base
allele
primer
specific
Prior art date
Application number
PCT/GB2002/000794
Other languages
English (en)
Other versions
WO2002068684A2 (fr
WO2002068684A8 (fr
Inventor
Joakim Lundeberg
Afshin Ahmadian
Paal Nyren
Original Assignee
Pyrosequencing Ab
Dzieglewska Hanna
Joakim Lundeberg
Afshin Ahmadian
Paal Nyren
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/791,190 external-priority patent/US20030104372A1/en
Priority claimed from GB0104560A external-priority patent/GB0104560D0/en
Application filed by Pyrosequencing Ab, Dzieglewska Hanna, Joakim Lundeberg, Afshin Ahmadian, Paal Nyren filed Critical Pyrosequencing Ab
Priority to AU2002233540A priority Critical patent/AU2002233540A1/en
Publication of WO2002068684A2 publication Critical patent/WO2002068684A2/fr
Publication of WO2002068684A8 publication Critical patent/WO2002068684A8/fr
Publication of WO2002068684A3 publication Critical patent/WO2002068684A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes d'extension d'amorce spécifique d'allèle utiles dans la détection de mutations et de variations génétiques. L'invention concerne, plus particulièrement, une méthode de détection d'une base au niveau d'une position prédéfinie dans une molécule d'acide nucléique. La méthode de l'invention consiste: à effectuer des réaction d'extension d'amorce au moyen d'amorces de détection à spécificité de base, chaque amorce étant spécifique pour une base particulière au niveau de la position prédéfinie, et à comparer ces extensions d'amorce afin de déterminer quelle base est présente au niveau de cette position, les réactions d'extension d'amorce étant effectuées au moyen de nucléotides marqués et une enzyme de dégradation de nucléotide étant présente durant la réaction d'extension d'amorce.
PCT/GB2002/000794 2001-02-23 2002-02-22 Méthode d'extension d'amorce spécifique d'allèle WO2002068684A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002233540A AU2002233540A1 (en) 2001-02-23 2002-02-22 Allele-specific primer extension assay

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB0104560.8 2001-02-23
US09/791,190 2001-02-23
US09/791,190 US20030104372A1 (en) 1996-12-23 2001-02-23 Allele specific primer extension
GB0104560A GB0104560D0 (en) 2001-02-23 2001-02-23 Method
US7192602A 2002-02-07 2002-02-07
US10/071,926 2002-02-07

Publications (3)

Publication Number Publication Date
WO2002068684A2 WO2002068684A2 (fr) 2002-09-06
WO2002068684A8 WO2002068684A8 (fr) 2003-12-04
WO2002068684A3 true WO2002068684A3 (fr) 2007-12-27

Family

ID=27256081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/000794 WO2002068684A2 (fr) 2001-02-23 2002-02-22 Méthode d'extension d'amorce spécifique d'allèle

Country Status (2)

Country Link
AU (1) AU2002233540A1 (fr)
WO (1) WO2002068684A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1620568T3 (da) 2003-04-24 2009-03-30 Afshin Ahmadian Analyse til allelspecifik mutationspåvisning
EP2298785B1 (fr) * 2004-02-06 2012-07-25 Canadian Blood Services Détermination simultanée de groupe sanguin et de génotypes d'antigène de plaquette
GB0600116D0 (en) * 2006-01-05 2006-02-15 Univ Cardiff Allele-specific sequencing
WO2011059285A2 (fr) 2009-11-16 2011-05-19 (주)지노믹트리 Méthode de génotypage
US9803236B2 (en) 2010-08-06 2017-10-31 Tsinghua University Microarray-based assay integrated with particles for analyzing molecular interactions
JP5872569B2 (ja) 2010-10-27 2016-03-01 キャピタルバイオ コーポレーションＣａｐｉｔａｌＢｉｏ Ｃｏｒｐｏｒａｔｉｏｎ マイクロアレイに基づくアッセイのための粒子と結合させた発光団で標識された分子
WO2013123031A2 (fr) * 2012-02-15 2013-08-22 Veridex, Llc Procédé extrêmement sensible de détection de mutations à faible fréquence
CN103760355B (zh) 2013-12-05 2015-09-16 博奥生物集团有限公司 微阵列芯片检测中核苷酸序列的颗粒标记方法
US11492666B2 (en) * 2017-02-15 2022-11-08 Pacific Biosciences Of California, Inc. Distinguishing sequences by detecting polymerase dissociation
US11634774B2 (en) * 2017-05-18 2023-04-25 Pharmacogenetics Limited Genome-wide capture of inter-transposable element segments for genomic sequence analysis of human DNA samples with microbial contamination
CN109371115A (zh) * 2018-08-24 2019-02-22 山东德诺生物科技有限公司 用于检测rs5918的引物探针组及其应用
ES2966013T3 (es) * 2018-09-25 2024-04-17 Co Diagnostics Inc Diseño específico de alelo de cebadores cooperativos para genotipado de variantes de ácido nucleico mejorado

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0530794A1 (fr) * 1991-09-06 1993-03-10 Roche Diagnostics GmbH Méthode pour la détection d'acides nucléiques ayant une séquence similaire
EP0593789A1 (fr) * 1992-05-08 1994-04-27 Sumitomo Metal Industries, Ltd. Methode de determination de la mutation pre-c du virus de l'hepatite b
WO1998028440A1 (fr) * 1996-12-23 1998-07-02 Pyrosequencing Ab Methode de sequençage d'adn sur la base de la detection d'une liberation de pyrophosphate
WO2000053812A2 (fr) * 1999-03-12 2000-09-14 President And Fellows Of Harvard College Amplification par replique de reseaux d'acides nucleiques
EP1061135A2 (fr) * 1999-06-17 2000-12-20 Becton Dickinson and Company Procédés et oligonucléotides pour la détection des variations des séquences d'acides nucléiques

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0530794A1 (fr) * 1991-09-06 1993-03-10 Roche Diagnostics GmbH Méthode pour la détection d'acides nucléiques ayant une séquence similaire
EP0593789A1 (fr) * 1992-05-08 1994-04-27 Sumitomo Metal Industries, Ltd. Methode de determination de la mutation pre-c du virus de l'hepatite b
WO1998028440A1 (fr) * 1996-12-23 1998-07-02 Pyrosequencing Ab Methode de sequençage d'adn sur la base de la detection d'une liberation de pyrophosphate
WO2000053812A2 (fr) * 1999-03-12 2000-09-14 President And Fellows Of Harvard College Amplification par replique de reseaux d'acides nucleiques
EP1061135A2 (fr) * 1999-06-17 2000-12-20 Becton Dickinson and Company Procédés et oligonucléotides pour la détection des variations des séquences d'acides nucléiques

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AHMADIAN A ET AL: "Genotyping by apyrase-mediated allele-specific extension.", NUCLEIC ACIDS RESEARCH. ENGLAND 15 DEC 2001, vol. 29, no. 24, 15 December 2001 (2001-12-15), pages E121, XP002236747, ISSN: 1362-4962 *
FAN J-B ET AL: "Parallel genotyping of human SNPs using generic high-density oligonucleotide tag arrays", GENOME RESEARCH 2000 UNITED STATES, vol. 10, no. 6, 2000, pages 853 - 860, XP002236784, ISSN: 1088-9051 *

Also Published As

Publication number Publication date
AU2002233540A8 (en) 2008-02-14
AU2002233540A1 (en) 2002-09-12
WO2002068684A2 (fr) 2002-09-06
WO2002068684A8 (fr) 2003-12-04

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