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WO2002067860A3 - Selection par dispositif d'affichage miroir - Google Patents

Selection par dispositif d'affichage miroir Download PDF

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Publication number
WO2002067860A3
WO2002067860A3 PCT/US2002/005193 US0205193W WO02067860A3 WO 2002067860 A3 WO2002067860 A3 WO 2002067860A3 US 0205193 W US0205193 W US 0205193W WO 02067860 A3 WO02067860 A3 WO 02067860A3
Authority
WO
WIPO (PCT)
Prior art keywords
selection
kdo
sugars
identified
naturally occurring
Prior art date
Application number
PCT/US2002/005193
Other languages
English (en)
Other versions
WO2002067860A2 (fr
Inventor
Chi-Huey Wong
Original Assignee
Scripps Research Inst
Chi-Huey Wong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst, Chi-Huey Wong filed Critical Scripps Research Inst
Priority to AU2002250155A priority Critical patent/AU2002250155A1/en
Publication of WO2002067860A2 publication Critical patent/WO2002067860A2/fr
Publication of WO2002067860A3 publication Critical patent/WO2002067860A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Selon l'invention, on identifie les liants artificiels se trouvant dans les glucides et les sucres des surfaces cellulaires au moyen d'un procédé de criblage qui utilise les énantiomères immobilisés de ces glucides et sucres de surfaces cellulaires. Les liants artificiels préférés comprennent des D-peptides et des L-acides nucléiques et résistent à la dégradation enzymatique et à l'épuration. Par ailleurs, ce procédé à permis d'identifier des séquences Fab à chaîne simple qui se lient à l'acide sialique et au KDO selon une affinité nano-molaire. Des exemples de procédures de criblage utilisent des D-KDO, L-acide sialique et L-sialo-disaccharide fixés sur un support solide pour sélectionner des liants à forte affinité.
PCT/US2002/005193 2001-02-22 2002-02-22 Selection par dispositif d'affichage miroir WO2002067860A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002250155A AU2002250155A1 (en) 2001-02-22 2002-02-22 Selection by mirror image display

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27137701P 2001-02-22 2001-02-22
US60/271,377 2001-02-22

Publications (2)

Publication Number Publication Date
WO2002067860A2 WO2002067860A2 (fr) 2002-09-06
WO2002067860A3 true WO2002067860A3 (fr) 2003-02-20

Family

ID=23035297

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/005193 WO2002067860A2 (fr) 2001-02-22 2002-02-22 Selection par dispositif d'affichage miroir

Country Status (2)

Country Link
AU (1) AU2002250155A1 (fr)
WO (1) WO2002067860A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2045326A1 (fr) * 2007-10-05 2009-04-08 Bayer CropScience AG Procédé d'identification du partenaire d'interaction d'un agent actif

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5162513A (en) * 1991-09-20 1992-11-10 The Scripps Research Institute L-isomeric sugars having formed stereogenic centers of R configuration: methods and compositions
WO1997035194A2 (fr) * 1996-03-21 1997-09-25 President And Fellows Of Harvard College Procede de criblage enantiomere et compositions pour ce procede
US5780221A (en) * 1995-05-03 1998-07-14 Whitehead Institute For Biomedical Research Identification of enantiomeric ligands
US5869461A (en) * 1995-03-16 1999-02-09 Yale University Reducing toxicity of L-nucleosides with D-nucleosides
US6114309A (en) * 1997-11-21 2000-09-05 Incara Research Laboratories Combinatorial library of moenomycin analogs and methods of producing same
US6153383A (en) * 1997-12-09 2000-11-28 Verdine; Gregory L. Synthetic transcriptional modulators and uses thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5162513A (en) * 1991-09-20 1992-11-10 The Scripps Research Institute L-isomeric sugars having formed stereogenic centers of R configuration: methods and compositions
US5869461A (en) * 1995-03-16 1999-02-09 Yale University Reducing toxicity of L-nucleosides with D-nucleosides
US5780221A (en) * 1995-05-03 1998-07-14 Whitehead Institute For Biomedical Research Identification of enantiomeric ligands
WO1997035194A2 (fr) * 1996-03-21 1997-09-25 President And Fellows Of Harvard College Procede de criblage enantiomere et compositions pour ce procede
US6114309A (en) * 1997-11-21 2000-09-05 Incara Research Laboratories Combinatorial library of moenomycin analogs and methods of producing same
US6153383A (en) * 1997-12-09 2000-11-28 Verdine; Gregory L. Synthetic transcriptional modulators and uses thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DOOLEY ET AL.: "An all D-amino acid opioid peptide with central analgesic activity from a combinatorial library", SCIENCE, vol. 266, 23 December 1994 (1994-12-23), pages 2019 - 2022, XP002955242 *
KLUBMANN ET AL.: "Mirror-image RNA that binds D-adenosine", NATURE BIOTECH., vol. 14, September 1996 (1996-09-01), pages 1112 - 1115, XP002955243 *
ROCHE ET AL.: "Binding affinity and site selectivity of daunomycin analogues", BIOCHEMISTRY, vol. 33, no. 4, 1994, pages 936 - 942, XP002955241 *
SCHUMACHER ET AL.: "Identification of D-peptide ligands through mirror-image phage display", SCIENCE, vol. 271, 29 March 1996 (1996-03-29), pages 1854 - 1857, XP000650829 *

Also Published As

Publication number Publication date
AU2002250155A1 (en) 2002-09-12
WO2002067860A2 (fr) 2002-09-06

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