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WO2002066660A3 - Verfahren zur identifizierung von substanzen mit herbizider wirkung - Google Patents

Verfahren zur identifizierung von substanzen mit herbizider wirkung Download PDF

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Publication number
WO2002066660A3
WO2002066660A3 PCT/EP2002/001466 EP0201466W WO02066660A3 WO 2002066660 A3 WO2002066660 A3 WO 2002066660A3 EP 0201466 W EP0201466 W EP 0201466W WO 02066660 A3 WO02066660 A3 WO 02066660A3
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WIPO (PCT)
Prior art keywords
relates
gene products
genes
vectors
nucleic acid
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PCT/EP2002/001466
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English (en)
French (fr)
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WO2002066660A2 (de
Inventor
Gunnar Plesch
Astrid Blau
Klaus Daeschner
Mathieu Klein
Original Assignee
Metanomics Gmbh & Co Kgaa
Gunnar Plesch
Astrid Blau
Klaus Daeschner
Mathieu Klein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10107843A external-priority patent/DE10107843A1/de
Priority claimed from DE10125537A external-priority patent/DE10125537A1/de
Application filed by Metanomics Gmbh & Co Kgaa, Gunnar Plesch, Astrid Blau, Klaus Daeschner, Mathieu Klein filed Critical Metanomics Gmbh & Co Kgaa
Priority to AU2002256619A priority Critical patent/AU2002256619A1/en
Priority to US10/467,962 priority patent/US20050246784A1/en
Priority to EP02726107A priority patent/EP1362059A2/de
Priority to CA002437937A priority patent/CA2437937A1/en
Publication of WO2002066660A2 publication Critical patent/WO2002066660A2/de
Publication of WO2002066660A3 publication Critical patent/WO2002066660A3/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18Testing for antimicrobial activity of a material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

In dieser Erfindung werden Gene und Verfahren zur Isolierung von Genen beschrieben, welche für Genprodukte kodieren, die bei Ausfall zu einem stark verzögertem Wachstum und/oder völligem Wachstumsstopp auf der Keimlingsebene von Arabidopsis thaliana führen. Die Erfindung beschreibt die Verwendung dieser Gene und dadurch kodierter Genprodukte für die Entdeckung neuer Herbizide. Darüber hinaus beschreibt diese Erfindung die Verwendung der hier identifizierten Gene und Genprodukte für die Herstellung von gegenüber Herbiziden resistenten Pflanzen. Die vorliegende Erfindung betrifft auch ein Verfahren zur Identifizierung von Substanzen mit herbizider Wirkung, Substanzen identifiziert nach diesem Verfahren sowie deren Verwendung als Herbizid. Die Erfindung betrifft weiterhin Nukleinsäurekonstrukte, Vektoren, Organismen enthaltend die Nukleinsäurekonstrukte und/oder Vektoren, speziell transgene Pflanzen enthaltend die Nukleinsäurekonstrukte und/oder Vektoren. Ausserdem betrifft die Erfindung ein Verfahren zur Identifizierung von Antagonisten und ihre Verwendung, Antikörper und Antisense-RNA-Moleküle sowie Mittel enthaltend eine herbizide Substanz isoliert nach den erfindungsgemässen Verfahren. Weiterhin betrifft die Erfindung Zusammensetzungen enthaltend den Antikörper, die Antisense-Nukleinsäure und/oder den Antagonisten.
PCT/EP2002/001466 2001-02-16 2002-02-13 Verfahren zur identifizierung von substanzen mit herbizider wirkung WO2002066660A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2002256619A AU2002256619A1 (en) 2001-02-16 2002-02-13 Method for identifying herbicidally active substances
US10/467,962 US20050246784A1 (en) 2001-02-16 2002-02-13 Method for identifying herbicidally active substances
EP02726107A EP1362059A2 (de) 2001-02-16 2002-02-13 Verfahren zur identifizierung von substanzen mit herbizider wirkung
CA002437937A CA2437937A1 (en) 2001-02-16 2002-02-13 Method for identifying herbicidally active substances

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10107843.9 2001-02-16
DE10107843A DE10107843A1 (de) 2001-02-16 2001-02-16 Verfahren zur Identifizierung von Substanzen mit herbizider Wirkung
DE10125537.3 2001-05-23
DE10125537A DE10125537A1 (de) 2001-05-23 2001-05-23 Verfahren zur Identifizierung von Substanzen mit herbizider Wirkung

Publications (2)

Publication Number Publication Date
WO2002066660A2 WO2002066660A2 (de) 2002-08-29
WO2002066660A3 true WO2002066660A3 (de) 2003-08-28

Family

ID=26008557

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/001466 WO2002066660A2 (de) 2001-02-16 2002-02-13 Verfahren zur identifizierung von substanzen mit herbizider wirkung

Country Status (6)

Country Link
US (1) US20050246784A1 (de)
EP (1) EP1362059A2 (de)
AR (1) AR032709A1 (de)
AU (1) AU2002256619A1 (de)
CA (1) CA2437937A1 (de)
WO (1) WO2002066660A2 (de)

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DE10238434A1 (de) * 2002-08-16 2004-04-15 Metanomics Gmbh & Co. Kgaa Verfahren zur Identifizierung von Substanzen mit herbizider Wirkung
WO2006067219A1 (en) * 2004-12-22 2006-06-29 Vib Vzw Methods and means to increase the amounts of carbohydrates in plants
WO2006133970A2 (en) * 2005-06-17 2006-12-21 Københavns Universitet Novel plants
US20090044291A1 (en) * 2006-08-31 2009-02-12 D-Helix Drought-resistant plants and method for producing the plants
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US9260490B2 (en) 2009-02-25 2016-02-16 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
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BR112014005795A2 (pt) * 2011-09-13 2020-12-08 Monsanto Technology Llc métodos de controle de plantas, de redução da expressão de um gene de hppd de uma planta, de preparação de um nucleotídeo, e de identificação de polinucleotídeos úteis na modulação da expressão do gene de hppd no tratamento externo de uma planta, composições e cassete de expressão microbiana
ES2645927T3 (es) 2011-09-13 2017-12-11 Monsanto Technology Llc Procedimientos y composiciones para el control de malezas
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US10240161B2 (en) 2012-05-24 2019-03-26 A.B. Seeds Ltd. Compositions and methods for silencing gene expression
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US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
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UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
WO2015108982A2 (en) 2014-01-15 2015-07-23 Monsanto Technology Llc Methods and compositions for weed control using epsps polynucleotides
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Title
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Also Published As

Publication number Publication date
CA2437937A1 (en) 2002-08-29
US20050246784A1 (en) 2005-11-03
AU2002256619A1 (en) 2002-09-04
WO2002066660A2 (de) 2002-08-29
EP1362059A2 (de) 2003-11-19
AR032709A1 (es) 2003-11-19

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