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WO2002056003A3 - Procede et formulations pour la separation de macromolecules biologiques - Google Patents

Procede et formulations pour la separation de macromolecules biologiques Download PDF

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Publication number
WO2002056003A3
WO2002056003A3 PCT/US2002/001132 US0201132W WO02056003A3 WO 2002056003 A3 WO2002056003 A3 WO 2002056003A3 US 0201132 W US0201132 W US 0201132W WO 02056003 A3 WO02056003 A3 WO 02056003A3
Authority
WO
WIPO (PCT)
Prior art keywords
separation
biological macromolecules
formulations
linear polyacrylamide
denaturant
Prior art date
Application number
PCT/US2002/001132
Other languages
English (en)
Other versions
WO2002056003A2 (fr
Inventor
Richard Hammond
Sam Chen
Marie C Ruiz-Martinez
Kendra Swirsding
Yi-Neng Wu
Jan F Simons
Bruce Taillon
Original Assignee
Curagen Corp
Richard Hammond
Sam Chen
Marie C Ruiz-Martinez
Kendra Swirsding
Yi-Neng Wu
Jan F Simons
Bruce Taillon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Curagen Corp, Richard Hammond, Sam Chen, Marie C Ruiz-Martinez, Kendra Swirsding, Yi-Neng Wu, Jan F Simons, Bruce Taillon filed Critical Curagen Corp
Priority to AU2002234252A priority Critical patent/AU2002234252A1/en
Publication of WO2002056003A2 publication Critical patent/WO2002056003A2/fr
Publication of WO2002056003A3 publication Critical patent/WO2002056003A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne des procédés et des formulations pour la séparation de macromolécules biologiques, telles que des polynucléotides.
PCT/US2002/001132 2001-01-12 2002-01-14 Procede et formulations pour la separation de macromolecules biologiques WO2002056003A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002234252A AU2002234252A1 (en) 2001-01-12 2002-01-14 Method and formulations for the separation of biological macromolecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26148901P 2001-01-12 2001-01-12
US60/261,489 2001-01-12

Publications (2)

Publication Number Publication Date
WO2002056003A2 WO2002056003A2 (fr) 2002-07-18
WO2002056003A3 true WO2002056003A3 (fr) 2003-05-15

Family

ID=22993523

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/001132 WO2002056003A2 (fr) 2001-01-12 2002-01-14 Procede et formulations pour la separation de macromolecules biologiques

Country Status (2)

Country Link
AU (1) AU2002234252A1 (fr)
WO (1) WO2002056003A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002951153A0 (en) * 2002-09-02 2002-09-19 Proteome Systems Intellectual Property Pty Ltd An electrophoresis gel having improved swelling properties
EP2362212B1 (fr) * 2009-03-18 2019-02-20 Agilent Technologies, Inc. PDMA et cellulose dérivatisée en tant que support de séparation pour ADN

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000028314A1 (fr) * 1998-11-10 2000-05-18 Curagen Corporation Procedes et formulations pour separer des macromolecules biologiques

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000028314A1 (fr) * 1998-11-10 2000-05-18 Curagen Corporation Procedes et formulations pour separer des macromolecules biologiques

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CARRILHO E ET AL: "Rapid DNA sequencing of more than 1000 bases per run by capillary electrophoresis using replaceable linear polyacrylamide solutions.", ANALYTICAL CHEMISTRY, vol. 68, no. 19, 1 October 1996 (1996-10-01), ISSN 0003-2700, pages 3305 - 3313, XP001088146 *
DATABASE MEDLINE [online] June 1996 (1996-06-01), WU C ET AL: "Polyacrylamide solutions for DNA sequencing by capillary electrophoresis: mesh sizes, separation and dispersion.", XP002210903, Database accession no. NLM8832178 *
ELECTROPHORESIS, vol. 17, no. 6, June 1996 (1996-06-01), ISSN 0173-0835, pages 1103 - 1109 *
GOETZINGER W ET AL: "Characterization of high molecular mass linear polyacrylamide powder prepared by emulsion polymerization as a replaceable polymer matrix for DNA sequencing by capillary electrophoresis", ELECTROPHORESIS, vol. 19, no. 2, 1998, ISSN 0173-0835, pages 242 - 248, XP000893173 *
SALAS-SOLANO O ET AL: "Optimization of high-performance DNA sequencing on short microfabricated electrophoretic devices.", ANALYTICAL CHEMISTRY, vol. 72, no. 14, 15 July 2000 (2000-07-15), ISSN 0003-2700, pages 3129 - 3137, XP002210902 *
SALAS-SOLANO O ET AL: "Routine DNA sequencing of 1000 bases in less than one hour by capillary electrophoresis with replaceable linear polyacrylamide solutions.", ANALYTICAL CHEMISTRY, vol. 70, no. 19, 1 October 1998 (1998-10-01), ISSN 0003-2700, pages 3996 - 4003, XP001088167 *

Also Published As

Publication number Publication date
AU2002234252A1 (en) 2002-07-24
WO2002056003A2 (fr) 2002-07-18

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