WO2002046457A3 - Procede de codage de sondes d'hybridation - Google Patents
Procede de codage de sondes d'hybridation Download PDFInfo
- Publication number
- WO2002046457A3 WO2002046457A3 PCT/EP2001/014391 EP0114391W WO0246457A3 WO 2002046457 A3 WO2002046457 A3 WO 2002046457A3 EP 0114391 W EP0114391 W EP 0114391W WO 0246457 A3 WO0246457 A3 WO 0246457A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- overhang
- encoders
- dna fragment
- attached
- encoder
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000009396 hybridization Methods 0.000 title 1
- 239000000523 sample Substances 0.000 title 1
- 239000012634 fragment Substances 0.000 abstract 4
- 230000000295 complement effect Effects 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Procédé d'identification d'acides nucléiques, caractérisé en ce qu'il comprend les étapes suivantes : (a) préparation d'au moins un fragment d'ADN ayant une extrémité pendante de longueur connue et de séquence inconnue, (b) fixation d'un codeur déterminé, formé d'un mélange de codeurs ayant une extrémité également pendante à l'extrémité pendante de la séquence inconnue du fragment d'ADN, l'extrémité pendante du codeur fixé et l'extrémité pendante de séquence inconnue du fragment d'ADN étant totalement complémentaires, (c) le cas échéant, séparation des codeurs qui, à l'étape (b), n'ont été fixés à aucun fragment d'ADN, (d) marquage du produit de fixation de l'étape (b), dans la mesure où des codeurs non déjà marqués pour la fixation ont été utilisés, (e) hybridation du produit de fixation marqué avec un ensemble d'oligonucléotides qui sont sensiblement complémentaires de l'un des brins de tous les codeurs possibles du mélange de codeurs ou d'une sélection de ceux-ci.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2002216078A AU2002216078A1 (en) | 2000-12-07 | 2001-12-07 | Method for encoding hybridization probes |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10060827A DE10060827A1 (de) | 2000-12-07 | 2000-12-07 | Verfahren zur Codierung von Hybridisierungssonden |
| DE10060827.2 | 2000-12-07 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2002046457A2 WO2002046457A2 (fr) | 2002-06-13 |
| WO2002046457A3 true WO2002046457A3 (fr) | 2002-12-19 |
Family
ID=7666146
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP2001/014391 WO2002046457A2 (fr) | 2000-12-07 | 2001-12-07 | Procede de codage de sondes d'hybridation |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU2002216078A1 (fr) |
| DE (1) | DE10060827A1 (fr) |
| WO (1) | WO2002046457A2 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115058469A (zh) * | 2022-04-25 | 2022-09-16 | 深圳大学 | 一种短dna片段3’端生物素标记的方法 |
| CN118072835B (zh) * | 2024-04-19 | 2024-09-17 | 宁波甬恒瑶瑶智能科技有限公司 | 基于机器学习的生物信息学数据处理方法、系统及介质 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5710000A (en) * | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
| WO1998010095A1 (fr) * | 1996-09-05 | 1998-03-12 | Brax Genomics Limited | Caracterisation d'un adn |
| WO2000009756A1 (fr) * | 1998-08-17 | 2000-02-24 | The Perkin-Elmer Corporation | Analyse d'expression dirigee a l'aide d'un adaptateur |
| WO2000060124A2 (fr) * | 1999-04-06 | 2000-10-12 | Yale University | Analyse d'adresses fixes de séquences étiquetées |
| WO2001012855A2 (fr) * | 1999-08-13 | 2001-02-22 | Yale University | Etiquette de sequence a codage binaire |
-
2000
- 2000-12-07 DE DE10060827A patent/DE10060827A1/de not_active Withdrawn
-
2001
- 2001-12-07 WO PCT/EP2001/014391 patent/WO2002046457A2/fr not_active Application Discontinuation
- 2001-12-07 AU AU2002216078A patent/AU2002216078A1/en not_active Abandoned
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5710000A (en) * | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
| WO1998010095A1 (fr) * | 1996-09-05 | 1998-03-12 | Brax Genomics Limited | Caracterisation d'un adn |
| WO2000009756A1 (fr) * | 1998-08-17 | 2000-02-24 | The Perkin-Elmer Corporation | Analyse d'expression dirigee a l'aide d'un adaptateur |
| WO2000060124A2 (fr) * | 1999-04-06 | 2000-10-12 | Yale University | Analyse d'adresses fixes de séquences étiquetées |
| WO2001012855A2 (fr) * | 1999-08-13 | 2001-02-22 | Yale University | Etiquette de sequence a codage binaire |
Non-Patent Citations (1)
| Title |
|---|
| BRENNER SYDNEY ET AL: "Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays", NATURE BIOTECHNOLOGY, NATURE PUB. CO, NEW YORK, NY, US, vol. 18, no. 6, June 2000 (2000-06-01), pages 630 - 634, XP002199492, ISSN: 1087-0156 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002216078A1 (en) | 2002-06-18 |
| DE10060827A1 (de) | 2002-06-13 |
| WO2002046457A2 (fr) | 2002-06-13 |
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