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WO2002041999A1 - Systeme de decondensation - Google Patents

Systeme de decondensation Download PDF

Info

Publication number
WO2002041999A1
WO2002041999A1 PCT/DK2001/000762 DK0100762W WO0241999A1 WO 2002041999 A1 WO2002041999 A1 WO 2002041999A1 DK 0100762 W DK0100762 W DK 0100762W WO 0241999 A1 WO0241999 A1 WO 0241999A1
Authority
WO
WIPO (PCT)
Prior art keywords
plate
heating
cooling
wells
cooling plate
Prior art date
Application number
PCT/DK2001/000762
Other languages
English (en)
Inventor
Per Nielsen
Flemming Stig Larsen
Original Assignee
Novo Nordisk A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk A/S filed Critical Novo Nordisk A/S
Priority to AU2002223503A priority Critical patent/AU2002223503A1/en
Publication of WO2002041999A1 publication Critical patent/WO2002041999A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25B—REFRIGERATION MACHINES, PLANTS OR SYSTEMS; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS
    • F25B47/00—Arrangements for preventing or removing deposits or corrosion, not provided for in another subclass
    • F25B47/006—Arrangements for preventing or removing deposits or corrosion, not provided for in another subclass for preventing frost
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25B—REFRIGERATION MACHINES, PLANTS OR SYSTEMS; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS
    • F25B21/00—Machines, plants or systems, using electric or magnetic effects
    • F25B21/02—Machines, plants or systems, using electric or magnetic effects using Peltier effect; using Nernst-Ettinghausen effect

Definitions

  • Method and apparatus for counteracting condense dew on a transparent cover film covering each well in an array of wells which forms a test plate having a lower side formed by the bot- toms of the wells and an upper side defined by the upper edges of the wells onto which edges the cover film is sealingly attached.
  • test plates comprising a large number of juxtaposed wells.
  • a compound to be examined is placed together with a reagent, which react with the compound if this compound has the wanted properties.
  • the reagent are chosen so that the reaction can be visually inspected, although this visual inspection is mostly made by a camera which photographs the content of the wells and produces images which can later be inspected by a human or a machine to select the wells containing useful compounds.
  • the visual inspection is performed by sensors receiving a light signal from the content of the well which signal may be immediately evaluated to decide whether the content of the inspected well is interesting or not.
  • a transparent cover film covering the wells and closing them hermetically is glued to the surface formed by the upper edges of the wells.
  • This cover film prevents evaporation of the liquid in the wells and prevents contamination of the content of the wells. Further it prevents contamination of the inspection apparatus if the plate is tilted sufficiently to make the liquid flow over the edges of the wells.
  • Blowing hot air over the film for a while may make the condense water evaporate to make the film transparent but the effect will only last for a time which is too short to allow the wanted inspection of the wells. If too hot air is blown over the film for more than just a while, the content of the wells or the well itself or its cover film may be damaged before an inspection can be completed. An alternating heating and inspection makes the inspection process slow and cumbersome and is unsuited for an automatic scanning process.
  • An objective of the invention therefore is to provide a method by which condense water on a transparent cover film can be prevented, removed and kept away for a while sufficient to perform an automatized inspection of all the wells on a test plate before disturbing dew formations occur.
  • test plate for a set time is placed with its lower surface contacting a cooling plate which is kept at a set temperature below the room temperature and with its upper surface in a distance from a heating plate kept at a set temperature higher than the room temperature.
  • the cooling plate is maintained in the temperature interval 0 - 10C° and the heating plate is held in the temperature interval 50 - 110C° preferably in the interval 60 - 75C° whereby temperatures, which may harm the samples in the wells, the wells or their cover film, or the gluing between well edge and film, are avoided.
  • the time the test plate stays between the cooling plate and the heating plate may be 1 - 10 minutes which has ap- peared to keep the covering film free from harmful condense for 30 minutes which is sufficient for a careful optical reading of the reactions in the individual wells.
  • the invention further relates to an apparatus by which the method can be performed.
  • Such an apparatus is characterise in that it comprises, a cooling plate on which a test plate can be placed, a heating plate parallel with the cooling plate in a distance from the cooling plate which distance is larger than the thickness of the testplate, cooling means maintaining the cooling plate at a set temperature lower than the room temperature, and heating means maintaining the heating plate at a set temperature over the room temperature.
  • the heating means may appropriately comprise an electric resistive heating element and a heat distributing plate to obtain an even heating all over the heating surface facing the upper side of the test plate.
  • the cooling plate may be cooled by a Peltier element, which provides solid state cooling without use of moving parts or flowing liquid.
  • the apparatus may be provided with means for setting the temperatures of the heating and the cooling plates, which means cooperating with feed back signals from the cooling and the heating plates controls a power supply so that a set temperature for the respective plates is maintained at a set value.
  • the heating and the cooling are provided in a reproducible way.
  • the cooling plate is shaped with a raised platform mating a depression in the bottom of the test plate. This way a safe positioning of the plate on the cooling element is obtained and the matching platform and depression ensure a good heat conductance between the cooling plate and the test plate.
  • the depression is found in common used test plates as it further serves the purpose of making the test plates suited for piling.
  • the space between the heating plate and the cooling plate may be ventilated to avoid dew on the cooling plate as any liquid on the outer side of the covered test plate is unwanted by the succeeding handling of the test plates.
  • Figure 1 shows a photography of a test plate with condense on the film covering the wells of the test plate
  • Figure 2 shows a photography of a test plate without condense on the film covering the wells of the test plate
  • Figure 3 shows schematically a sectional side view of an apparatus according to the invention with a test plate positioned in said apparatus
  • Figure 4 shows schematically and end side view of an apparatus according to the invention.
  • Figure 5 shows schematically a front side view of the apparatus shown in figure 4.
  • Figure 1 shows a test plate comprising an array of wells wherein samples are placed and inspected through a transparent film covering the wells to protect their content against contamination and drying out and further ensuring that the content of the wells are not spilt if the plate is tilted during the handling.
  • the cover film which is glued to the edges of the partitions between the wells, the air between the samples and the cover film will be saturated with vapour which will condense on the cover film as small droplets which reflects the light and makes the inspection window appear as a white matt surface so that no precise visual inspection can be made.
  • the condensed droplets make the covered open end of the wells appear as white fields in figure 1.
  • Figure 2 shows a test plate as the one shown in figure 2 but seen before liquid has con- densed on the cover film.
  • the film over the wells remains transparent so that the well can be looked into. This makes the areas over the wells appear as black.
  • the white markings in figure 2 originate from reflections in the upper edges of the partitions, which separate the wells.
  • the temperature of this film must be raised over the dew point for the vapour in the well. This may be obtained by blowing hot air over the plate and the cover film.
  • the heat capacity of the film is rather low so its temperature quickly returns to the dew point when the test plate is removed from the heating station and inserted in the inspection apparatus.
  • Figure 3 shows schematically an apparatus for treating a test plate in advance of inspection of the wells.
  • the test plate 1 has an array of wells 2 which are covered by a cover film 3, which adheres to the edges of the wells and seals each of the wells 1.
  • the apparatus comprises a heating plate 4 in or on the upper side of which an electric resistance heating element 7 is installed, and a cooling plate 5 which is cooled by a Peltier ele- ment 8 mounted with its cool side in contact with the cooling plate, whereas its warm side is provided with not shown cooling ribs.
  • the cooling plate 5 is provided with a raised platform 6 with oblique edges, which platform 6 mates a depression in the bottom of the test plate so that this test plate is secured against horizontal movements on the cooling plate 5.
  • a control box 9 has a power input line 10 and contains a controlling circuit controlling the power supply through a line 11 to the heating element 7 and through a line 12 to the cooling element 8 on the basis of temperature signals from the heating plate 4 and from the cooling plate 5 through lines 15 and 16, respectively, and of the settings of a pair of temperature setting buttons 13 and 14 for the heating plate and the cooling plate, respectively.
  • the distance between the cooling plate and the heating plate can be made adjustable.
  • Figure 4 and 5 shows schematically an decondensing device with a heating plate 4 placed in a distance over a cooling plate 5 leaving a space so that a test plate may be placed on the cooling plate 5 beneath the heating plate 4.
  • the cooling and heating plates are held in distance of each other by a wall 18 comprising one or more small fans which can blow room temperature air over the cooling plate to keep it free from dew.
  • the heating plate is heated by heaters build into the heating plate whereas the cooling plate 5 is cooled by a Peltier element 8 which are cooled by cooling ribs beneath the cooling plate.
  • Legs 19 support the whole apparatus.
  • test plates made ready for reading may be stored I piles from which they are taken by a robot an carried to the decon- denser apparatus in which they are inserted for a set time where after they are again taken by the robot and inserted in an imager, which automatically performs a reading of the light from the wells.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Mechanical Engineering (AREA)
  • Thermal Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention a pour objet de s'opposer à la condensation de la vapeur d'eau sur un film de couverture transparent (3) recouvrant chaque cupule (2) d'un ensemble de cupules formant une plaque témoin (1). Cette plaque comporte un côté inférieur formé par les fonds des cupules (2) et un côté supérieur formé par les bords supérieurs de ces cupules (2) sur lesquels le film de couverture (3) est fixé avec étanchéité. La plaque témoin (1) est placée de telle sorte que sa surface inférieure est en contact avec une plaque de refroidissement (5) qui est conservée à une température déterminée inférieure à la température ambiante par un élément de refroidissement (8) et sa surface supérieure se trouve à une distance donnée d'une plaque de chauffage (4) qui est maintenue par un élément de chauffage (7) à une température déterminée supérieure à la température ambiante. Un boîtier de commande (9) qui reçoit des signaux de rétroaction provenant de la plaque de refroidissement (5) et de la plaque de chauffage (4) commande l'alimentation électrique des éléments de chauffage et de refroidissement (7, 8) pour maintenir les températures des plaques de chauffage et de refroidissement (4, 5) déterminées par des boutons de réglage de température (13 et 14).
PCT/DK2001/000762 2000-11-24 2001-11-16 Systeme de decondensation WO2002041999A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002223503A AU2002223503A1 (en) 2000-11-24 2001-11-16 Decondenser unit

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200001771 2000-11-24
DKPA200001771 2000-11-24

Publications (1)

Publication Number Publication Date
WO2002041999A1 true WO2002041999A1 (fr) 2002-05-30

Family

ID=8159866

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2001/000762 WO2002041999A1 (fr) 2000-11-24 2001-11-16 Systeme de decondensation

Country Status (2)

Country Link
AU (1) AU2002223503A1 (fr)
WO (1) WO2002041999A1 (fr)

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004009239A1 (fr) * 2002-07-23 2004-01-29 Applera Corporation Couvercle pour ensemble plaque chauffante
GB2404882A (en) * 2002-11-19 2005-02-16 Biogene Ltd Microtitre plate with heating means to prevent condensation
WO2007045644A1 (fr) * 2005-10-17 2007-04-26 Novo Nordisk A/S Unite de reservoir ventilee pour medicaments
EP2255010A1 (fr) * 2008-02-20 2010-12-01 Streck Inc. Thermocycleur et récipient à échantillons pour l'amplification rapide de l'adn
US8062253B2 (en) 2003-10-21 2011-11-22 Novo Nordisk A/S Medical skin mountable device
US8298172B2 (en) 2005-04-13 2012-10-30 Novo Nordisk A/S Medical skin mountable device and system
ITTO20110990A1 (it) * 2011-10-28 2013-04-29 Silicon Biosystems Spa Metodo ed apparato per l'analisi ottica di particelle a basse temperature
US20140213487A1 (en) * 2011-09-30 2014-07-31 Life Technologies Corporation Systems and Methods for Biological Analysis
US8992754B2 (en) 2005-10-26 2015-03-31 Silicon Biosystems S.P.A. Method and apparatus for the characterizing and counting particles, in particular, biological particles
US9581528B2 (en) 2006-03-27 2017-02-28 Menarini Silicon Biosystems S.P.A. Method and apparatus for the processing and/or analysis and/or selection of particles, in particular, biological particles
US9592336B2 (en) 2003-10-27 2017-03-14 Novo Nordisk A/S Medical skin mountable device
US9719960B2 (en) 2005-07-19 2017-08-01 Menarini Silicon Biosystems S.P.A. Method and apparatus for the manipulation and/or the detection of particles
US9737891B2 (en) 2011-06-01 2017-08-22 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
US10006861B2 (en) 2013-06-28 2018-06-26 Streck, Inc. Devices for real-time polymerase chain reaction
US10234447B2 (en) 2008-11-04 2019-03-19 Menarini Silicon Biosystems S.P.A. Method for identification, selection and analysis of tumour cells
US10376878B2 (en) 2011-12-28 2019-08-13 Menarini Silicon Biosystems S.P.A. Devices, apparatus, kit and method for treating a biological sample
US10648897B2 (en) 2007-10-29 2020-05-12 Menarini Silicon Biosystems S.P.A. Method and apparatus for the identification and handling of particles
US10895575B2 (en) 2008-11-04 2021-01-19 Menarini Silicon Biosystems S.P.A. Method for identification, selection and analysis of tumour cells
CN113164958A (zh) * 2018-12-06 2021-07-23 耶拿分析仪器有限公司 用于微量滴定板的盖子

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5552580A (en) * 1989-12-22 1996-09-03 Beckman Instruments, Inc. Heated cover device
WO1998043740A2 (fr) * 1997-03-28 1998-10-08 The Perkin-Elmer Corporation Ameliorations apportees a un cycleur thermique pour pcr
WO2000061797A1 (fr) * 1999-04-08 2000-10-19 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Thermocycleur rapide a enceinte chauffante

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5552580A (en) * 1989-12-22 1996-09-03 Beckman Instruments, Inc. Heated cover device
WO1998043740A2 (fr) * 1997-03-28 1998-10-08 The Perkin-Elmer Corporation Ameliorations apportees a un cycleur thermique pour pcr
WO2000061797A1 (fr) * 1999-04-08 2000-10-19 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Thermocycleur rapide a enceinte chauffante

Cited By (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7560273B2 (en) 2002-07-23 2009-07-14 Applied Biosystems, Llc Slip cover for heated platen assembly
WO2004009239A1 (fr) * 2002-07-23 2004-01-29 Applera Corporation Couvercle pour ensemble plaque chauffante
GB2404882A (en) * 2002-11-19 2005-02-16 Biogene Ltd Microtitre plate with heating means to prevent condensation
US8062253B2 (en) 2003-10-21 2011-11-22 Novo Nordisk A/S Medical skin mountable device
US9592336B2 (en) 2003-10-27 2017-03-14 Novo Nordisk A/S Medical skin mountable device
US8747363B2 (en) 2005-04-13 2014-06-10 Novo Nordisk A/S Medical skin mountable device and system
US8298172B2 (en) 2005-04-13 2012-10-30 Novo Nordisk A/S Medical skin mountable device and system
US9719960B2 (en) 2005-07-19 2017-08-01 Menarini Silicon Biosystems S.P.A. Method and apparatus for the manipulation and/or the detection of particles
WO2007045644A1 (fr) * 2005-10-17 2007-04-26 Novo Nordisk A/S Unite de reservoir ventilee pour medicaments
US8394060B2 (en) 2005-10-17 2013-03-12 Novo Nordisk A/S Vented drug reservoir unit
US8992754B2 (en) 2005-10-26 2015-03-31 Silicon Biosystems S.P.A. Method and apparatus for the characterizing and counting particles, in particular, biological particles
US10092904B2 (en) 2006-03-27 2018-10-09 Menarini Silicon Biosystems S.P.A. Method and apparatus for the processing and/or analysis and/or selection of particles, in particular biological particles
US9581528B2 (en) 2006-03-27 2017-02-28 Menarini Silicon Biosystems S.P.A. Method and apparatus for the processing and/or analysis and/or selection of particles, in particular, biological particles
US10648897B2 (en) 2007-10-29 2020-05-12 Menarini Silicon Biosystems S.P.A. Method and apparatus for the identification and handling of particles
EP2255010A4 (fr) * 2008-02-20 2011-09-21 Streck Inc Thermocycleur et récipient à échantillons pour l'amplification rapide de l'adn
EP2255010A1 (fr) * 2008-02-20 2010-12-01 Streck Inc. Thermocycleur et récipient à échantillons pour l'amplification rapide de l'adn
US10895575B2 (en) 2008-11-04 2021-01-19 Menarini Silicon Biosystems S.P.A. Method for identification, selection and analysis of tumour cells
US12153040B2 (en) 2008-11-04 2024-11-26 Menarini Silicon Biosystems S.P.A. Method for identification, selection and analysis of tumour cells
US10234447B2 (en) 2008-11-04 2019-03-19 Menarini Silicon Biosystems S.P.A. Method for identification, selection and analysis of tumour cells
US9737891B2 (en) 2011-06-01 2017-08-22 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
US10323274B2 (en) * 2011-09-30 2019-06-18 Life Technologies Corporation Systems and methods for biological analysis
US20140213487A1 (en) * 2011-09-30 2014-07-31 Life Technologies Corporation Systems and Methods for Biological Analysis
US11339425B2 (en) 2011-09-30 2022-05-24 Life Technologies Corporation Systems and methods for biological analysis
CN103907009A (zh) * 2011-10-28 2014-07-02 硅生物系统股份公司 用于低温下的粒子的光学分析的方法和设备
KR102000541B1 (ko) 2011-10-28 2019-07-16 메나리니 실리콘 바이오시스템스 에스.피.에이. 저온에서의 입자들의 광학 분석을 위한 방법 및 장치
KR20140103259A (ko) * 2011-10-28 2014-08-26 실리콘 바이오시스템스 에스.피.에이. 저온에서의 입자들의 광학 분석을 위한 방법 및 장치
WO2013061312A1 (fr) * 2011-10-28 2013-05-02 Silicon Biosystems S.P.A. Procédé et dispositif pour analyse optique de particules à basses températures
ITTO20110990A1 (it) * 2011-10-28 2013-04-29 Silicon Biosystems Spa Metodo ed apparato per l'analisi ottica di particelle a basse temperature
US20210080374A1 (en) * 2011-10-28 2021-03-18 Menarini Silicon Biosystems S.P.A. Method and device for optical analysis of particles at low temperatures
US11921028B2 (en) * 2011-10-28 2024-03-05 Menarini Silicon Biosystems S.P.A. Method and device for optical analysis of particles at low temperatures
US10376878B2 (en) 2011-12-28 2019-08-13 Menarini Silicon Biosystems S.P.A. Devices, apparatus, kit and method for treating a biological sample
US11385178B2 (en) 2013-06-28 2022-07-12 Streck, Inc. Devices for real-time polymerase chain reaction
US11953438B2 (en) 2013-06-28 2024-04-09 Streck Llc Devices for real-time polymerase chain reaction
US10006861B2 (en) 2013-06-28 2018-06-26 Streck, Inc. Devices for real-time polymerase chain reaction
CN113164958B (zh) * 2018-12-06 2023-10-13 耶拿分析仪器有限两合公司 用于微量滴定板的盖子
CN113164958A (zh) * 2018-12-06 2021-07-23 耶拿分析仪器有限公司 用于微量滴定板的盖子

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