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WO2002040710A3 - Verfahren zur detektion von methylierungszuständen zur toxikologischen diagnostik - Google Patents

Verfahren zur detektion von methylierungszuständen zur toxikologischen diagnostik Download PDF

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Publication number
WO2002040710A3
WO2002040710A3 PCT/EP2001/012951 EP0112951W WO0240710A3 WO 2002040710 A3 WO2002040710 A3 WO 2002040710A3 EP 0112951 W EP0112951 W EP 0112951W WO 0240710 A3 WO0240710 A3 WO 0240710A3
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WIPO (PCT)
Prior art keywords
toxicological
diagnostic
determined
sample
methylation states
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Application number
PCT/EP2001/012951
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English (en)
French (fr)
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WO2002040710A2 (de
Inventor
Alexander Olek
Christian Piepenbrock
Kurt Berlin
Original Assignee
Epigenomics Ag
Alexander Olek
Christian Piepenbrock
Kurt Berlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag, Alexander Olek, Christian Piepenbrock, Kurt Berlin filed Critical Epigenomics Ag
Priority to AU2002223672A priority Critical patent/AU2002223672A1/en
Priority to EP01996625A priority patent/EP1337668A2/de
Priority to US10/416,905 priority patent/US20040048279A1/en
Priority to JP2002543021A priority patent/JP2004513650A/ja
Publication of WO2002040710A2 publication Critical patent/WO2002040710A2/de
Publication of WO2002040710A3 publication Critical patent/WO2002040710A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/142—Toxicological screening, e.g. expression profiles which identify toxicity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur toxikologischen Diagnostik. Einem Lebewesen oder einer Zellkultur, die zuvor einer bestimmten auf ihre toxikologische Wirkung zu untersuchende Substanz ausgesetzt wurden, wird eine DNA-Probe entnommen. Die in dieser Probe enthaltene DNA wird chemisch vorbehandelt und die Basenabfolge eines Teils der modifizierten DNA bestimmt. Daraus wird auf einen für die Probe charakteristischen Methylierungszustand oder ein charakteristisches Methylierungsmuster geschlossen. Durch den Abgleich mit Daten aus Methylierungszuständen anderer Proben wird auf die Einwirkung einer Substanz auf das Lebewesen oder die Zellkultur geschlossen und/oder mit anderen Substanzen in toxikologischer Hinsicht verglichen.
PCT/EP2001/012951 2000-11-14 2001-11-08 Verfahren zur detektion von methylierungszuständen zur toxikologischen diagnostik WO2002040710A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2002223672A AU2002223672A1 (en) 2000-11-14 2001-11-08 Method for detecting methylation states for a toxicological diagnostic
EP01996625A EP1337668A2 (de) 2000-11-14 2001-11-08 Verfahren zur detektion von methylierungszuständen zur toxikologischen diagnostik
US10/416,905 US20040048279A1 (en) 2000-11-14 2001-11-08 Method for detecting methylation states for a toxicological diagnostic
JP2002543021A JP2004513650A (ja) 2000-11-14 2001-11-08 メチル化状態を検出することによる毒物学的診断方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10056802.5 2000-11-14
DE10056802A DE10056802B4 (de) 2000-11-14 2000-11-14 Verfahren zur Detektion von Methylierungszuständen zur toxikologischen Diagnostik

Publications (2)

Publication Number Publication Date
WO2002040710A2 WO2002040710A2 (de) 2002-05-23
WO2002040710A3 true WO2002040710A3 (de) 2003-05-30

Family

ID=7663517

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/012951 WO2002040710A2 (de) 2000-11-14 2001-11-08 Verfahren zur detektion von methylierungszuständen zur toxikologischen diagnostik

Country Status (6)

Country Link
US (1) US20040048279A1 (de)
EP (1) EP1337668A2 (de)
JP (1) JP2004513650A (de)
AU (1) AU2002223672A1 (de)
DE (1) DE10056802B4 (de)
WO (1) WO2002040710A2 (de)

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GB0215509D0 (en) * 2002-07-04 2002-08-14 Novartis Ag Marker genes
EP1428889A1 (de) * 2002-12-10 2004-06-16 Epigenomics AG Methode der Überwachung des Übergangs einer Zelle von einem Zustand in einen Anderen
US8415100B2 (en) 2003-08-14 2013-04-09 Case Western Reserve University Methods and compositions for detecting gastrointestinal and other cancers
US7485420B2 (en) * 2003-08-14 2009-02-03 Case Western Reserve University Methods and compositions for detecting colon cancers
EP2078098A2 (de) * 2006-11-01 2009-07-15 Epigenomics AG Verfahren, systeme und computerprogrammprodukte zur bestimmung von biomarkern für das ansprechen auf eine behandlung
JP5211790B2 (ja) * 2007-03-26 2013-06-12 住友化学株式会社 Ｄｎａメチル化測定方法
WO2010037001A2 (en) 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
DE102010031354B3 (de) * 2010-07-14 2012-02-02 Technische Universität Dresden Mittel und Verfahren zur Diagnostik, Prognose und Therapiekontrolle von Tumorerkrankungen
WO2013090588A1 (en) 2011-12-13 2013-06-20 Oslo Universitetssykehus Hf Methods and kits for detection of methylation status
US10563248B2 (en) 2012-11-30 2020-02-18 Cambridge Epigenetix Limited Oxidizing agent for modified nucleotides
KR101302173B1 (ko) 2012-12-07 2013-08-30 이화여자대학교 산학협력단 Ｈｍｏｘ１ 유전자의 메틸화 변화를 이용한 알츠하이머 질환의 진단용 조성물 및 이를 이용한 알츠하이머 질환의 진단방법
US9169509B2 (en) 2013-01-15 2015-10-27 Board Of Regents, The University Of Texas System Topoisomerase 2b as a predictor of susceptibility to anthracycline-induced cardiotoxicity
US20160039877A1 (en) 2013-03-15 2016-02-11 Shenzhen Hightide Biopharmaceutical, Ltd. Compositions and methods of using islet neogenesis peptides and analogs thereof
KR102390107B1 (ko) 2013-05-30 2022-04-25 그라함 에이치. 크리시 국부 신경 자극
US11229789B2 (en) 2013-05-30 2022-01-25 Neurostim Oab, Inc. Neuro activator with controller
WO2015069965A1 (en) * 2013-11-08 2015-05-14 Veterinary Diagnostics Institute, Inc. Method and apparatus for detecting vector-borne diseases in mammals
US11077301B2 (en) 2015-02-21 2021-08-03 NeurostimOAB, Inc. Topical nerve stimulator and sensor for bladder control
US11459573B2 (en) 2015-09-30 2022-10-04 Trustees Of Boston University Deadman and passcode microbial kill switches
CN107177679A (zh) * 2017-06-07 2017-09-19 北京呈诺医学科技有限公司 一种检测食管鳞癌相关基因tfpi2和hspb2启动子甲基化程度的试剂盒
EP3706856A4 (de) 2017-11-07 2021-08-18 Neurostim Oab, Inc. Nicht-invasiver nervenaktivator mit adaptiver schaltung
WO2020264214A1 (en) 2019-06-26 2020-12-30 Neurostim Technologies Llc Non-invasive nerve activator with adaptive circuit
WO2021126921A1 (en) 2019-12-16 2021-06-24 Neurostim Solutions, Llc Non-invasive nerve activator with boosted charge delivery
AU2021319150A1 (en) 2020-07-30 2023-03-02 Cambridge Epigenetix Limited Compositions and methods for nucleic acid analysis
CN114878840A (zh) * 2022-07-12 2022-08-09 昆明思安生物科技有限公司 磁微粒化学发光测定三碘甲状腺原氨酸试剂盒及检测方法
CN118685521A (zh) * 2024-08-26 2024-09-24 湖南宏雅基因技术有限公司 一种用于前列腺癌检测的引物探针组合物及其应用

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Also Published As

Publication number Publication date
US20040048279A1 (en) 2004-03-11
EP1337668A2 (de) 2003-08-27
DE10056802A1 (de) 2002-05-29
DE10056802B4 (de) 2005-06-16
AU2002223672A1 (en) 2002-05-27
WO2002040710A2 (de) 2002-05-23
JP2004513650A (ja) 2004-05-13

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