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WO2001029239A3 - Modified resistance genes - Google Patents

Modified resistance genes Download PDF

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Publication number
WO2001029239A3
WO2001029239A3 PCT/GB2000/003930 GB0003930W WO0129239A3 WO 2001029239 A3 WO2001029239 A3 WO 2001029239A3 GB 0003930 W GB0003930 W GB 0003930W WO 0129239 A3 WO0129239 A3 WO 0129239A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
elicitor
auto
processes
activator
Prior art date
Application number
PCT/GB2000/003930
Other languages
French (fr)
Other versions
WO2001029239A2 (en
Inventor
Abdelhafid Bendahmane
David Charles Baulcombe
Original Assignee
Plant Bioscience Ltd
Abdelhafid Bendahmane
David Charles Baulcombe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Bioscience Ltd, Abdelhafid Bendahmane, David Charles Baulcombe filed Critical Plant Bioscience Ltd
Priority to EP00968085A priority Critical patent/EP1228225A2/en
Priority to AU78043/00A priority patent/AU7804300A/en
Publication of WO2001029239A2 publication Critical patent/WO2001029239A2/en
Publication of WO2001029239A3 publication Critical patent/WO2001029239A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Disclosed are processes for modifying the activation characteristics of a first polypeptide having an amino acid sequence which includes a nucleotide binding site (NBS) and a leucine rich repeat (LRR) domain, which first polypeptide mediates a cellular response leading to pathogen resistance and/or cell death or dysfunction in response to an elicitor (e.g. apoptosis regulator or plant resistance polypeptide such as Rx), the process comprising the step of introducing a modification to the amino acid sequence of the first polypeptide such as to produce an auto-activator polypeptide which is capable of activation in the absence of the elicitor. Preferred modifications include those which decreases the net negative charge of the NB-ARC region, particularly in or around GLPL, CFLY or MHD motifs. Other preferred modifications are such that the auto-activator polypeptide is artificially dimerized, or is dimerized under predefined conditions in response to a dimerizing effector agent which is not the elicitor. Also disclosed are processes for producing such auto-activator polypeptides and nucleic acids encoding the same, plus various methods and materials for use in such processes, and methods of using the products e.g. for influencing or affecting a cellular response in a plant.
PCT/GB2000/003930 1999-10-15 2000-10-12 Modified resistance genes WO2001029239A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00968085A EP1228225A2 (en) 1999-10-15 2000-10-12 Modified resistance genes
AU78043/00A AU7804300A (en) 1999-10-15 2000-10-12 Modified resistance genes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9924483.2 1999-10-15
GBGB9924483.2A GB9924483D0 (en) 1999-10-15 1999-10-15 Modified resistance genes

Publications (2)

Publication Number Publication Date
WO2001029239A2 WO2001029239A2 (en) 2001-04-26
WO2001029239A3 true WO2001029239A3 (en) 2002-06-13

Family

ID=10862838

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/003930 WO2001029239A2 (en) 1999-10-15 2000-10-12 Modified resistance genes

Country Status (4)

Country Link
EP (1) EP1228225A2 (en)
AU (1) AU7804300A (en)
GB (1) GB9924483D0 (en)
WO (1) WO2001029239A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1496122B1 (en) * 2002-03-27 2010-12-29 University of Tsukuba Novel meloidogyne-resistance gene and utilization thereof
DE102005026045A1 (en) * 2005-06-03 2007-06-14 Kws Saat Ag Nucleic acid encoding an auto-activated resistance protein for producing resistance to pathogens in plants
WO2014127835A1 (en) 2013-02-22 2014-08-28 Christian-Albrechts-Universität Zu Kiel Plant-derived resistance gene

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999038989A1 (en) * 1998-01-30 1999-08-05 The Regents Of The University Of California Constitutively active plant disease resistance genes and polypeptides
WO1999041258A1 (en) * 1998-02-13 1999-08-19 President And Fellows Of Harvard College Novel dimerizing agents, their production and use
WO1999045118A1 (en) * 1998-03-06 1999-09-10 Commonwealth Scientific And Industrial Research Organisation Genetic sequences conferring pathogen resistance in plants and uses therefor
WO1999045129A1 (en) * 1998-03-06 1999-09-10 Mogen International N.V. Method for the induction of pathogen resistance in plants
WO1999054490A2 (en) * 1998-04-16 1999-10-28 Plant Bioscience Limited Plant-derived resistance gene

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999038989A1 (en) * 1998-01-30 1999-08-05 The Regents Of The University Of California Constitutively active plant disease resistance genes and polypeptides
WO1999041258A1 (en) * 1998-02-13 1999-08-19 President And Fellows Of Harvard College Novel dimerizing agents, their production and use
WO1999045118A1 (en) * 1998-03-06 1999-09-10 Commonwealth Scientific And Industrial Research Organisation Genetic sequences conferring pathogen resistance in plants and uses therefor
WO1999045129A1 (en) * 1998-03-06 1999-09-10 Mogen International N.V. Method for the induction of pathogen resistance in plants
WO1999054490A2 (en) * 1998-04-16 1999-10-28 Plant Bioscience Limited Plant-derived resistance gene

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BENDAHMANE ET AL: "the Rx gene from potato controls separate virus resistance and cell death responses", PLANT CELL,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD,US, vol. 11, May 1999 (1999-05-01), pages 781 - 791, XP002129096, ISSN: 1040-4651 *
BIEZEN VAN DER E A ET AL: "THE NB-ARC DOMAIN: A NOVEL SIGNALLING MOTIF SHARED BY PLANT RESISTANCE GENE PRODUCTS AND REGULATORS OF CELL DEATH IN ANIMALS", CURRENT BIOLOGY,CURRENT SCIENCE,,GB, vol. 8, no. 7, 26 March 1998 (1998-03-26), pages R226 - R227, XP000924862, ISSN: 0960-9822 *
COLLINS NICHOLAS ET AL: "Molecular characterization of the maize Rp1-D rust resistance haplotype and its mutants", PLANT CELL,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD,US, vol. 11, no. 7, July 1999 (1999-07-01), pages 1365 - 1376, XP002164372, ISSN: 1040-4651 *
DATABASE EMBL 13 July 1999 (1999-07-13) *
RATHJEN JOHN P ET AL: "Constitutively active Pto induces a Prf-dependent hypersensitive response in the absence of avrPto.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 18, no. 12, 15 June 1999 (1999-06-15), pages 3232 - 3240, XP002167939, ISSN: 0261-4189 *
VAN DER BIEZEN ERIC ET AL: "Plant disease-resistance proteins and the gene-for-gene concept", TIBS TRENDS IN BIOCHEMICAL SCIENCES,ELSEVIER PUBLICATION, CAMBRIDGE,EN, vol. 23, no. 12, December 1998 (1998-12-01), pages 454 - 456, XP002158209, ISSN: 0968-0004 *

Also Published As

Publication number Publication date
AU7804300A (en) 2001-04-30
EP1228225A2 (en) 2002-08-07
WO2001029239A2 (en) 2001-04-26
GB9924483D0 (en) 1999-12-15

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