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WO2001022052A1 - Ensemble d'appareils destine a la preparation et l'analyse de tissus pour examens microscopiques - Google Patents

Ensemble d'appareils destine a la preparation et l'analyse de tissus pour examens microscopiques Download PDF

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Publication number
WO2001022052A1
WO2001022052A1 PCT/DE2000/003365 DE0003365W WO0122052A1 WO 2001022052 A1 WO2001022052 A1 WO 2001022052A1 DE 0003365 W DE0003365 W DE 0003365W WO 0122052 A1 WO0122052 A1 WO 0122052A1
Authority
WO
WIPO (PCT)
Prior art keywords
containers
reagents
device arrangement
arrangement according
container
Prior art date
Application number
PCT/DE2000/003365
Other languages
German (de)
English (en)
Inventor
Aurel Popa
Original Assignee
Aurel Popa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aurel Popa filed Critical Aurel Popa
Priority to EP00967611A priority Critical patent/EP1218719A1/fr
Publication of WO2001022052A1 publication Critical patent/WO2001022052A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates

Definitions

  • the invention relates to a device arrangement for cells or tissues of living beings which are to be examined cytologically, histologically or immunologically.
  • the device arrangement is particularly suitable for immunohistochemical detection of proteins and of mRNA.
  • the appropriate pretreatment is an essential prerequisite for microscopic evaluation of the tissues. After death, living cells and tissues are first autolytically fermentative, then bacterially decomposed. A treatment that prevents this decomposition is therefore necessary to examine their structures. This fixation should largely preserve living structures so that they can be assessed in real life. Depending on the objective of the investigation, further treatments such as washing, dyeing, incubating with antibodies, etc., are required after the tissue section has been produced. In the subsequent microscopic examination, it is necessary to place the tissue section on a slide. [State of the art]
  • DE OS 3 313 127 proposes a device for coloring biological samples, in which the tissue section is introduced into a microscope carrier m and a container is brought into contact with the liquid. The device is fed and removed by hand; further treatments are not possible with this device.
  • Fully automatic systems are also known (DE PS 3634976). These consist of a housing with several containers with the appropriate reagents and a specimen holder transport device. This dips the object carrier with the tissue sections into the container, the time duration and sequence being predetermined by a control system. All known devices use a slide, this enables the necessary handling of the samples. But the devices are by the transport or gripping elements ⁇ correspondingly expensive. Since the samples are treated one after the other, the guarantee of the same treatment conditions is not completely guaranteed.
  • the object of the invention is to develop a device arrangement without complicated mechanics, which with the treatment of a large number of free-floating tissue sections different reagents and ensures equal treatment.
  • the invention proceeds from the known principle that the tissue sections are fed to the individual reagents.
  • the idea of the invention is to direct the reagents in batches of tissue sections.
  • a device arrangement is proposed which enables free storage of the tissue sections and the arbitrary supply of reagents.
  • combinable containers for different batch sizes and reticular spacers are proposed.
  • the nets not only enable handling and spacing of the tissue sections, but also bring about wetting and winding around the surface.
  • tissue sections can be treated in less time with less effort.
  • the different conditions required in each case are optimally maintained.
  • the same treatment of the tissue sections e batch is ensured, which is particularly important in comparative studies.
  • the device arrangement is shown in principle in the figure.
  • Several cylinder-shaped containers (1) are screwed one above the other to form a washing column.
  • the containers (1) each have an internal or external thread on the circumference and possibly one Poetry. They are made of appropriate plastic, such as polyethylene.
  • the top and bottom container (1) each have a bottom plate and hose connections.
  • the tissue sections (2) are located horizontally in the containers (1), lying on nylon nets (3).
  • the washing column is connected to a pump (4) and storage containers (6) for reagent liquids via hoses, a two-way valve (5) and a three-way valve (5).
  • a sequence control not shown, controls the valves (5) and pump (4) in a predetermined sequence.
  • the arrangement of the device enables rinsing to collect the used washing liquid and circulation of reagent liquids. With additional valves (5) and storage containers (6) this can be expanded according to the requirements. Function in immunochemistry:
  • the tissue sections (2) are placed in the container (1), a nylon mesh (3) with a diameter of 31 mm being placed between the individual tissue sections (2). After each feed a further container (1) is screwed and at the end a container (1) with the conclusion ⁇ ground and hose Connections.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un ensemble d'appareils servant à l'examen cytologique, histologique ou immunologique de cellules ou de tissus d'êtres vivants. Cet ensemble d'appareils est particulièrement approprié pour la détection immunohistochimique de protéines et d'ARNm. L'invention vise à développer un ensemble d'appareils dépourvu de mécanismes compliqués, permettant de traiter un grand nombre d'échantillons de tissus, flottant librement, avec différents réactifs afin d'assurer un traitement homogène. A cet effet, au moins deux contenants (1) servant à recevoir des échantillons de tissus (2) communiquent l'un avec l'autre et peuvent être reliés de manière étanche aux liquides par rapport à l'environnement. De plus, les échantillons de tissus sont maintenus dans les contenants en étant séparés par des moyens d'écartement (3) et peuvent être librement traversés par un fluide. Les contenants (1) possèdent un raccord pour l'arrivée et l'évacuation des réactifs et sont reliés, par l'intermédiaire d'une pompe (4) et de soupapes (5), à des réservoirs de stockage (6) pour les réactifs de sorte que ces derniers puissent circuler en circuit fermé dans les contenants ou bien traversent simplement ces derniers dans un ordre quelconque.
PCT/DE2000/003365 1999-09-23 2000-09-21 Ensemble d'appareils destine a la preparation et l'analyse de tissus pour examens microscopiques WO2001022052A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP00967611A EP1218719A1 (fr) 1999-09-23 2000-09-21 Ensemble d'appareils destine a la preparation et l'analyse de tissus pour examens microscopiques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19945621.6 1999-09-23
DE1999145621 DE19945621A1 (de) 1999-09-23 1999-09-23 Geräteanordnung zur Präparation und Analyse von Gewebe für mirkoskopische Untersuchungen

Publications (1)

Publication Number Publication Date
WO2001022052A1 true WO2001022052A1 (fr) 2001-03-29

Family

ID=7923049

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/003365 WO2001022052A1 (fr) 1999-09-23 2000-09-21 Ensemble d'appareils destine a la preparation et l'analyse de tissus pour examens microscopiques

Country Status (3)

Country Link
EP (1) EP1218719A1 (fr)
DE (1) DE19945621A1 (fr)
WO (1) WO2001022052A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10149345A1 (de) * 2001-10-06 2003-05-08 Leica Mikrosysteme Gmbh Wien Vorrichtung und Verfahren zur schnellen Gewebepräparation für Histologische Untersuchungen
CN110132695A (zh) * 2019-05-27 2019-08-16 福州迈新生物技术开发有限公司 一种病理染色系统降低人为失误所致染色异常方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10163488A1 (de) * 2001-12-21 2003-07-10 Microm Int Gmbh Vorrichtung zur Behandlung von histologischen Proben

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3635013A1 (de) * 1986-10-15 1988-05-11 Graskoetter Karl Rainer Vorrichtung zur untersuchung von in vitro gehaltenen gewebeproben
US4847208A (en) * 1987-07-29 1989-07-11 Bogen Steven A Apparatus for immunohistochemical staining and method of rinsing a plurality of slides
US5231029A (en) * 1989-08-23 1993-07-27 Royal Postgraduate Medical School Apparatus for the in situ hybridization of slide-mounted cell samples
WO1999009390A1 (fr) * 1997-08-20 1999-02-25 The University Of Miami Procede d'impregnation, de suppression de la graisse, de deshydratation et de fixation de tissus, continu et de haute qualite

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3635013A1 (de) * 1986-10-15 1988-05-11 Graskoetter Karl Rainer Vorrichtung zur untersuchung von in vitro gehaltenen gewebeproben
US4847208A (en) * 1987-07-29 1989-07-11 Bogen Steven A Apparatus for immunohistochemical staining and method of rinsing a plurality of slides
US5231029A (en) * 1989-08-23 1993-07-27 Royal Postgraduate Medical School Apparatus for the in situ hybridization of slide-mounted cell samples
WO1999009390A1 (fr) * 1997-08-20 1999-02-25 The University Of Miami Procede d'impregnation, de suppression de la graisse, de deshydratation et de fixation de tissus, continu et de haute qualite

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10149345A1 (de) * 2001-10-06 2003-05-08 Leica Mikrosysteme Gmbh Wien Vorrichtung und Verfahren zur schnellen Gewebepräparation für Histologische Untersuchungen
DE10149345B4 (de) * 2001-10-06 2007-11-15 Leica Mikrosysteme Gmbh Vorrichtung zur schnellen Gewebepräparation für histologische Untersuchungen
US7514042B2 (en) 2001-10-06 2009-04-07 Leica Mikrosysteme Gmbh Device and method for rapid tissue preparations for histological investigations
CN110132695A (zh) * 2019-05-27 2019-08-16 福州迈新生物技术开发有限公司 一种病理染色系统降低人为失误所致染色异常方法
CN110132695B (zh) * 2019-05-27 2022-01-11 福州迈新生物技术开发有限公司 一种病理染色系统降低人为失误所致染色异常方法

Also Published As

Publication number Publication date
DE19945621A1 (de) 2001-04-05
EP1218719A1 (fr) 2002-07-03

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