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WO2001018189A3 - Method for expressing exogenous sequences in mammalian cells - Google Patents

Method for expressing exogenous sequences in mammalian cells Download PDF

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Publication number
WO2001018189A3
WO2001018189A3 PCT/EP2000/008563 EP0008563W WO0118189A3 WO 2001018189 A3 WO2001018189 A3 WO 2001018189A3 EP 0008563 W EP0008563 W EP 0008563W WO 0118189 A3 WO0118189 A3 WO 0118189A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequences
mammalian cells
relates
nucleus
expressing exogenous
Prior art date
Application number
PCT/EP2000/008563
Other languages
German (de)
French (fr)
Other versions
WO2001018189A2 (en
WO2001018189A9 (en
Inventor
Daniele Zink
Original Assignee
Daniele Zink
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daniele Zink filed Critical Daniele Zink
Priority to AU74158/00A priority Critical patent/AU7415800A/en
Publication of WO2001018189A2 publication Critical patent/WO2001018189A2/en
Publication of WO2001018189A3 publication Critical patent/WO2001018189A3/en
Publication of WO2001018189A9 publication Critical patent/WO2001018189A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for expressing exogenous sequences in mammalian cells depending on the functional architecture of the genome in the nucleus of the mammalian cell. The invention specifically relates to a method for expressing transgenic and proviral sequences in mammalian cells, wherein the sequences are specifically localized in the active compartment of the nucleus of the mammalian cell. The invention further relates to a method for expressing exogenous sequences in mammalian cells, wherein the sequences are specifically integrated into the DNA of the inactive compartment of the nucleus of the mammalian cells. The invention also relates to a method for identifying regulatory sequences or other sequences that promote a stable localization or integration of sequences to be expressed in the active compartments of the nucleus of a mammalian cell. Also, the invention relates to the use of the inventive method for producing transgenic animals, for producing tissues and organs for the purpose of transplantation and to the use of the inventive method in gene therapy.
PCT/EP2000/008563 1999-09-03 2000-09-01 Method for expressing exogenous sequences in mammalian cells WO2001018189A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU74158/00A AU7415800A (en) 1999-09-03 2000-09-01 Method for expressing exogenous sequences in mammalian cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1999142149 DE19942149A1 (en) 1999-09-03 1999-09-03 Process for the expression of exogenous sequences in mammalian cells
DE19942149.8 1999-09-03

Publications (3)

Publication Number Publication Date
WO2001018189A2 WO2001018189A2 (en) 2001-03-15
WO2001018189A3 true WO2001018189A3 (en) 2001-09-27
WO2001018189A9 WO2001018189A9 (en) 2002-09-06

Family

ID=7920733

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/008563 WO2001018189A2 (en) 1999-09-03 2000-09-01 Method for expressing exogenous sequences in mammalian cells

Country Status (3)

Country Link
AU (1) AU7415800A (en)
DE (1) DE19942149A1 (en)
WO (1) WO2001018189A2 (en)

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001800A1 (en) * 1990-07-20 1992-02-06 Chiron Corporation Method for integrative transformation of yeast using dispersed repetitive elements
EP0592836A1 (en) * 1992-09-17 1994-04-20 American Cyanamid Company Human adeno-associated virus integration site DNA and uses thereof
WO1994013273A1 (en) * 1992-12-08 1994-06-23 The Trustees Of The University Of Pennsylvania Sequences useful for obtaining high level, copy number-dependent, essentially position-independent gene expression in cellular and transgenic systems
WO1995033841A1 (en) * 1994-06-09 1995-12-14 Medical Research Council Locus control subregions for integration-site independent transgene expression
WO1996019573A1 (en) * 1994-12-19 1996-06-27 Cangene Corporation Recombinant dna molecules and expression vectors for erythropoietin
WO1997040183A2 (en) * 1996-04-10 1997-10-30 The Biological Research Center Of The Hungarian Academy Of Sciences Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
WO1997046687A1 (en) * 1996-06-06 1997-12-11 Novartis Ag Vectors comprising sar elements
WO1997046691A1 (en) * 1996-06-03 1997-12-11 Universite Laval Integration cassette for improvement of transgenesis in eukaryotes
WO1998007876A2 (en) * 1996-08-16 1998-02-26 Medical Research Council Self-replicating episomal expression vectors conferring tissue-specific gene expression
WO2000077176A1 (en) * 1999-06-14 2000-12-21 North Carolina State University Method for obtaining low copy transgenes by direct dna transformation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9622084D0 (en) * 1996-10-23 1996-12-18 Isis Innovation Transinduction
US6245974B1 (en) * 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001800A1 (en) * 1990-07-20 1992-02-06 Chiron Corporation Method for integrative transformation of yeast using dispersed repetitive elements
EP0592836A1 (en) * 1992-09-17 1994-04-20 American Cyanamid Company Human adeno-associated virus integration site DNA and uses thereof
WO1994013273A1 (en) * 1992-12-08 1994-06-23 The Trustees Of The University Of Pennsylvania Sequences useful for obtaining high level, copy number-dependent, essentially position-independent gene expression in cellular and transgenic systems
WO1995033841A1 (en) * 1994-06-09 1995-12-14 Medical Research Council Locus control subregions for integration-site independent transgene expression
WO1996019573A1 (en) * 1994-12-19 1996-06-27 Cangene Corporation Recombinant dna molecules and expression vectors for erythropoietin
WO1997040183A2 (en) * 1996-04-10 1997-10-30 The Biological Research Center Of The Hungarian Academy Of Sciences Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
WO1997046691A1 (en) * 1996-06-03 1997-12-11 Universite Laval Integration cassette for improvement of transgenesis in eukaryotes
WO1997046687A1 (en) * 1996-06-06 1997-12-11 Novartis Ag Vectors comprising sar elements
WO1998007876A2 (en) * 1996-08-16 1998-02-26 Medical Research Council Self-replicating episomal expression vectors conferring tissue-specific gene expression
WO2000077176A1 (en) * 1999-06-14 2000-12-21 North Carolina State University Method for obtaining low copy transgenes by direct dna transformation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BROWN K ET AL: "Association of transcriptionally silent genes with Ikaros complexes at centromeric heterochromatin", CELL, vol. 91, no. 6, 12 December 1997 (1997-12-12), pages 845 - 854, XP002162572 *
MCKNIGHT R ET AL: "Matrix-attachment regions can impart position-independent regulation of a tissue-specific gene in transgenic mice", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 89, no. 15, 1 August 1992 (1992-08-01), pages 6943 - 6947, XP002162570 *
MCKNIGHT R ET AL: "Severe position effects imposed on a 1 kb mouse whey acidic protein gene promoter are overcome by heterologous matrix attachment regions", MOL REPROD DEV., vol. 44, no. 2, June 1996 (1996-06-01), pages 179 - 184, XP002162569 *
SADONI N ET AL: "Nuclear organization of mammalian genomes. Polar chromosome territories build up functionally distinct higher order compartments", THE JOURNAL OF CELL BIOLOGY., vol. 146, no. 6, 20 September 1999 (1999-09-20), pages 1211 - 1226, XP002162571 *

Also Published As

Publication number Publication date
DE19942149A1 (en) 2001-03-15
WO2001018189A2 (en) 2001-03-15
AU7415800A (en) 2001-04-10
WO2001018189A9 (en) 2002-09-06

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