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WO2001012847A3 - Isolation de cellules dans des echantillons judiciaires pour empreintes genetiques - Google Patents

Isolation de cellules dans des echantillons judiciaires pour empreintes genetiques Download PDF

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Publication number
WO2001012847A3
WO2001012847A3 PCT/EP2000/007771 EP0007771W WO0112847A3 WO 2001012847 A3 WO2001012847 A3 WO 2001012847A3 EP 0007771 W EP0007771 W EP 0007771W WO 0112847 A3 WO0112847 A3 WO 0112847A3
Authority
WO
WIPO (PCT)
Prior art keywords
specific
cells
present
relates
dna
Prior art date
Application number
PCT/EP2000/007771
Other languages
English (en)
Other versions
WO2001012847A2 (fr
Inventor
Den Eeckhout Elfride Van
Dieter Deforce
Original Assignee
Univ Gent
Den Eeckhout Elfride Van
Dieter Deforce
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Gent, Den Eeckhout Elfride Van, Dieter Deforce filed Critical Univ Gent
Priority to AU67012/00A priority Critical patent/AU6701200A/en
Publication of WO2001012847A2 publication Critical patent/WO2001012847A2/fr
Publication of WO2001012847A3 publication Critical patent/WO2001012847A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2833—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne une molécule spécifique à l'espèce, à la cellule ou à l'individu, à utiliser dans un procédé destiné à isoler des cellules dans un échantillon judiciaire, ladite molécule étant, de préférence, liée à une phase solide. Plus précisément, ladite molécule est un anticorps monoclonal ou polyclonal. L'invention traite également d'un procédé permettant d'isoler des cellules dans un échantillon judiciaire, lesdites cellules étant capturées à l'aide de ladite molécule. Elle traite en outre de procédés de préparation et d'analyse d'ADN à partir d'un tel échantillon. Elle concerne enfin des procédés pour isoler des cellules spécifiques dans des mélanges de cellules présentes dans un échantillon judiciaire.
PCT/EP2000/007771 1999-08-11 2000-08-10 Isolation de cellules dans des echantillons judiciaires pour empreintes genetiques WO2001012847A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU67012/00A AU6701200A (en) 1999-08-11 2000-08-10 Isolation of cells from forensic samples for dna-typing

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP99870173.4 1999-08-11
EP99870173 1999-08-11
US19929400P 2000-04-24 2000-04-24
US60/199,294 2000-04-24

Publications (2)

Publication Number Publication Date
WO2001012847A2 WO2001012847A2 (fr) 2001-02-22
WO2001012847A3 true WO2001012847A3 (fr) 2001-08-30

Family

ID=26153851

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/007771 WO2001012847A2 (fr) 1999-08-11 2000-08-10 Isolation de cellules dans des echantillons judiciaires pour empreintes genetiques

Country Status (2)

Country Link
AU (1) AU6701200A (fr)
WO (1) WO2001012847A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7320891B2 (en) 2004-09-10 2008-01-22 Promega Corporation Methods and kits for isolating sperm cells
US8993292B2 (en) 2007-01-16 2015-03-31 Applied Biosystems Llc Methods and systems for differential extraction
CN103592432B (zh) * 2013-10-15 2015-06-03 重庆市公安局 一种免疫磁珠分离精子与上皮细胞混合斑中精子的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0323829A2 (fr) * 1988-01-04 1989-07-12 E.I. Du Pont De Nemours And Company Procédé de séparation par affinité en étapes multiples pour isoler des cellules spécifiques à partir d'un mélange de cellules
WO1992013969A1 (fr) * 1991-01-31 1992-08-20 Baylor College Of Medicine Typage de l'adn au moyen des polymorphismes de courts segments a repetitions en tandem et identification de ces courts segments a repetitions en tandem polymorphiques

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0323829A2 (fr) * 1988-01-04 1989-07-12 E.I. Du Pont De Nemours And Company Procédé de séparation par affinité en étapes multiples pour isoler des cellules spécifiques à partir d'un mélange de cellules
WO1992013969A1 (fr) * 1991-01-31 1992-08-20 Baylor College Of Medicine Typage de l'adn au moyen des polymorphismes de courts segments a repetitions en tandem et identification de ces courts segments a repetitions en tandem polymorphiques

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
BISHARA A AND BRAUTBAR C: "A sensitive and specific ELISA using monoclonal capture antibodies for the detection of the HLA antigens in blood stains", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 120, 1989, pages 99 - 103, XP002128643 *
BISHARA A AND BRAUTBAR C: "Detection and identification of HLA antigens in dried spleens by means of enzyme linked immunosorbent assay (ELISA)", FORENSIC SCIENCE INTERNATIONAL, vol. 45, 1990, pages 97 - 105, XP000869791 *
BUTLER J M ET AL: "Forensic applications of mitochondrial DNA", TRENDS IN BIOTECHNOLOGY,GB,ELSEVIER PUBLICATIONS, CAMBRIDGE, vol. 16, no. 4, pages 158-162, XP004112301 *
CATTANEO C ET AL.: "Comparison of three DNA extraction methods on bone and blood stains up to 43 years old and amplification of three different gene sequences", JOURNAL OF FORENSIC SCIENCE, vol. 42, no. 6, 1997, pages 1126 - 1135, XP000869687 *
DIXON S C ET AL.: "Methods for extracting and amplifying genomic DNA isolated from frozen serum", NATURE BIOTECHNOLOGY, vol. 16, 1998, pages 91 - 94, XP002128645 *
GEE A P ET AL.: "T lymphozyte depletion of human micropheral blood and bone marrow using monoclonal antibodies and magnetic microspheres", BONE MARROW TRANSPLANTATION, vol. 2, no. 2, 1987, pages 155 - 163, XP000869902 *
GREENSPOON S A ET AL.: "QIAamp spin columns as a method of DNA isolation for forensic casework", JOURNAL OF FORENSIC SCIENCES, vol. 43, no. 5, 1998, pages 1024 - 1030, XP000974645 *
HANSEN T AND HANNESTAD K: "Direct HLA typing by rosetting with immunomagnetic beads coated with specific antibodies", JOURNAL OF IMMUNOGENETICS, vol. 16, no. 2, 1989, pages 137 - 139, XP000869908 *
MATTSSON L ET AL.: "Isolation of cell populations from arterial tissue, using monoclonal antibodies and magnetic microspheres", ATHEROSCLEROSIS, vol. 89, no. 1, 1991, pages 25 - 34, XP002128644 *
VARTDAL F ET AL.: "HLA class I and II typing using cells positively selected from blood by immunomagnetic isolation - a fast and reliable technique", TISSUE ANTIGENS, vol. 28, 1986, pages 301 - 312, XP000867222 *
WAYE J S ET AL: "A SIMPLE AND SENSITIVE METHOD FOR QUANTIFYING HUMAN GENOMIC DNA IN FORENSIC SPECIMEN EXTRACTS", BIOTECHNIQUES,US,NATICK, MA, vol. 7, no. 8, pages 852-855, XP000652990 *
ZIEGLER B L ET AL.: "Analysis of gene expression in small numbers of purified hemopoietic progenitor cells by RT-PCR", STEM CELLS, vol. 13, no. Sup1, 1995, pages 106 - 116, XP000870268 *

Also Published As

Publication number Publication date
AU6701200A (en) 2001-03-13
WO2001012847A2 (fr) 2001-02-22

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