+

WO2001011341A3 - A system for cell-based screening - Google Patents

A system for cell-based screening Download PDF

Info

Publication number
WO2001011341A3
WO2001011341A3 PCT/US2000/021426 US0021426W WO0111341A3 WO 2001011341 A3 WO2001011341 A3 WO 2001011341A3 US 0021426 W US0021426 W US 0021426W WO 0111341 A3 WO0111341 A3 WO 0111341A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
cell
based screening
kits
screens
Prior art date
Application number
PCT/US2000/021426
Other languages
French (fr)
Other versions
WO2001011341A2 (en
Inventor
Keith Olson
Oleg Lapets
Gary Bright
Randall O Shopoff
Original Assignee
Cellomics Inc
Keith Olson
Oleg Lapets
Gary Bright
Randall O Shopoff
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc, Keith Olson, Oleg Lapets, Gary Bright, Randall O Shopoff filed Critical Cellomics Inc
Priority to AU66228/00A priority Critical patent/AU6622800A/en
Publication of WO2001011341A2 publication Critical patent/WO2001011341A2/en
Publication of WO2001011341A3 publication Critical patent/WO2001011341A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
    • G01N33/5079—Mitochondria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The present invention provides systems, methods, screens, reagents and kits for optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect the mitotic index of cells.
PCT/US2000/021426 1999-08-05 2000-08-04 A system for cell-based screening WO2001011341A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU66228/00A AU6622800A (en) 1999-08-05 2000-08-04 A system for cell-based screening

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US14725499P 1999-08-05 1999-08-05
US60/147,254 1999-08-05
US39896599A 1999-09-17 1999-09-17
US09/398,965 1999-09-17
US17435600P 2000-01-04 2000-01-04
US60/174,356 2000-01-04
US17671500P 2000-01-18 2000-01-18
US60/176,715 2000-01-18

Publications (2)

Publication Number Publication Date
WO2001011341A2 WO2001011341A2 (en) 2001-02-15
WO2001011341A3 true WO2001011341A3 (en) 2001-07-05

Family

ID=27495751

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/021426 WO2001011341A2 (en) 1999-08-05 2000-08-04 A system for cell-based screening

Country Status (2)

Country Link
AU (1) AU6622800A (en)
WO (1) WO2001011341A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8953866B2 (en) 2004-03-16 2015-02-10 Amnis Corporation Method for imaging and differential analysis of cells

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8885913B2 (en) 1999-01-25 2014-11-11 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8131053B2 (en) 1999-01-25 2012-03-06 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8406498B2 (en) 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US7450229B2 (en) 1999-01-25 2008-11-11 Amnis Corporation Methods for analyzing inter-cellular phenomena
US6986993B1 (en) 1999-08-05 2006-01-17 Cellomics, Inc. System for cell-based screening
WO2001042786A2 (en) 1999-12-09 2001-06-14 Cellomics, Inc. System for cell based screening : cell spreading
AU2002230415A1 (en) 2000-11-17 2002-05-27 Universal Imaging Corporation Rapidly changing dichroic beamsplitter
WO2002061423A2 (en) 2000-12-22 2002-08-08 Cellomics, Inc. Identification of individual cells during kinetic assays
US7907765B2 (en) 2001-03-28 2011-03-15 University Of Washington Focal plane tracking for optical microtomography
US6636623B2 (en) * 2001-08-10 2003-10-21 Visiongate, Inc. Optical projection imaging system and method for automatically detecting cells with molecular marker compartmentalization associated with malignancy and disease
US7738945B2 (en) 2002-04-19 2010-06-15 University Of Washington Method and apparatus for pseudo-projection formation for optical tomography
US7260253B2 (en) 2002-04-19 2007-08-21 Visiongate, Inc. Method for correction of relative object-detector motion between successive views
US7369696B2 (en) * 2003-04-02 2008-05-06 Ge Healthcare Uk Limited Classification of cells into subpopulations using cell classifying data
GB0307684D0 (en) * 2003-04-02 2003-05-07 Amersham Biosciences Uk Ltd Determining cell cycle phase data
EP1800124B1 (en) 2004-03-16 2011-12-21 Amnis Corporation Image based quantitation of molecular translocation
EP2027465A2 (en) 2006-05-17 2009-02-25 Cellumen, Inc. Method for automated tissue analysis
JP4855139B2 (en) 2006-05-18 2012-01-18 オリンパス株式会社 Microscope device and cell observation method
US7835561B2 (en) 2007-05-18 2010-11-16 Visiongate, Inc. Method for image processing and reconstruction of images for optical tomography
US7787112B2 (en) * 2007-10-22 2010-08-31 Visiongate, Inc. Depth of field extension for optical tomography
EP2112509A1 (en) * 2008-03-25 2009-10-28 4Sc Ag Centrosome-assay
AU2012229102B2 (en) 2011-03-17 2016-02-04 Cernostics, Inc. Systems and compositions for diagnosing Barrett's esophagus and methods of using the same
US11069054B2 (en) 2015-12-30 2021-07-20 Visiongate, Inc. System and method for automated detection and monitoring of dysplasia and administration of immunotherapy and chemotherapy

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
WO2000003246A2 (en) * 1998-07-13 2000-01-20 Cellomics, Inc. A system for cell-based screening
WO2000017643A2 (en) * 1998-09-18 2000-03-30 Cellomics, Inc. A system for cell-based screening
WO2000050872A2 (en) * 1999-02-26 2000-08-31 Cellomics, Inc. A system for cell-based screening
WO2000070342A2 (en) * 1999-05-14 2000-11-23 Cellomics, Inc. Optical system analysis of cells

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
WO2000003246A2 (en) * 1998-07-13 2000-01-20 Cellomics, Inc. A system for cell-based screening
WO2000017643A2 (en) * 1998-09-18 2000-03-30 Cellomics, Inc. A system for cell-based screening
WO2000050872A2 (en) * 1999-02-26 2000-08-31 Cellomics, Inc. A system for cell-based screening
WO2000070342A2 (en) * 1999-05-14 2000-11-23 Cellomics, Inc. Optical system analysis of cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HENDZEL MICHAEL J ET AL: "Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation.", CHROMOSOMA (BERLIN), vol. 106, no. 6, November 1997 (1997-11-01), pages 348 - 360, XP000980606, ISSN: 0009-5915 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8953866B2 (en) 2004-03-16 2015-02-10 Amnis Corporation Method for imaging and differential analysis of cells

Also Published As

Publication number Publication date
WO2001011341A2 (en) 2001-02-15
AU6622800A (en) 2001-03-05

Similar Documents

Publication Publication Date Title
WO2000026408A3 (en) A system for cell-based screening
WO2000050872A3 (en) A system for cell-based screening
WO2001011341A3 (en) A system for cell-based screening
CA2381344A1 (en) Optical system analysis of cells
WO2000017643A3 (en) A system for cell-based screening
CA2394821A1 (en) Cell arrays and the uses thereof
DE69835537D1 (en) HIGH-FLOW SCREENING SYSTEM USING PLANAR FIXED PHASES
CA2282658A1 (en) A system for cell-based screening
WO2001042786A3 (en) System for cell based screening : cell spreading
EP1002233A4 (en) High-throughput assay
AU8132194A (en) Single nucleotide polymorphisms and their use in genetic analysis
WO2001086296A3 (en) Highly multiplexed reporter carrier systems
DE59814441D1 (en) USE OF CONTROL SURFACES FOR THE DETECTION OF DISTURBANCES IN A PROOF PROCESS
EP0977035A3 (en) A system for determining the electrical potential across a membrane
WO2002035260A3 (en) Light detection device
WO2000007017A3 (en) Method of high-throughput screening
AU7709198A (en) Low background multi-well plates for fluorescence measurements of biological andbiochemical samples
AU2561400A (en) Chemical and biochemical assay method and apparatus
WO2000070342A3 (en) Optical system analysis of cells
WO1999050459A3 (en) High throughput in vitro screening assays for transcription modulators
HUP0003261A2 (en) Multiple functionalities within an array element and uses thereof
WO1997040379A3 (en) Assays and reagents for identifying modulators of cdc25-mediated mitotic activation
ATE325190T1 (en) GENETIC SCREENING METHODS
WO2004013607A3 (en) Methods, devices and kits for multiplex blotting of biological samples from multi-well plates
BR9708256A (en) Process for identifying genes with specific expression to the site or preferred site in specific labeling cells present in a cell environment different or not from that of its origin and use

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载