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WO2001007577A3 - Protease conjugates having sterically protected epitope regions - Google Patents

Protease conjugates having sterically protected epitope regions Download PDF

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Publication number
WO2001007577A3
WO2001007577A3 PCT/US2000/018854 US0018854W WO0107577A3 WO 2001007577 A3 WO2001007577 A3 WO 2001007577A3 US 0018854 W US0018854 W US 0018854W WO 0107577 A3 WO0107577 A3 WO 0107577A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
conjugates
moiety
epitope regions
protease conjugates
Prior art date
Application number
PCT/US2000/018854
Other languages
French (fr)
Other versions
WO2001007577A2 (en
Inventor
Donn Nelton Rubingh
David John Weisgerber
Paul Elliott Correa
Original Assignee
Procter & Gamble
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter & Gamble filed Critical Procter & Gamble
Priority to MXPA02000837A priority Critical patent/MXPA02000837A/en
Priority to AU59283/00A priority patent/AU777550B2/en
Priority to BR0012692-6A priority patent/BR0012692A/en
Priority to EP00945317A priority patent/EP1196547A2/en
Priority to KR1020027000933A priority patent/KR20020021396A/en
Priority to CA002379723A priority patent/CA2379723A1/en
Priority to JP2001512848A priority patent/JP2003505069A/en
Publication of WO2001007577A2 publication Critical patent/WO2001007577A2/en
Publication of WO2001007577A3 publication Critical patent/WO2001007577A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)

Abstract

The present disclosure relates to subtilisin protease conjugate comprising a protease moiety and one or more addition moieties. Each addition moiety is covalently attached to an epitope protection position of the protease moiety. The protease conjugates have decreased immunogenicity relative to a parent protease. The present disclosure further relates to cleaning and personal care compositions comprising the protease conjugates.
PCT/US2000/018854 1999-07-22 2000-07-11 Protease conjugates having sterically protected epitope regions WO2001007577A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
MXPA02000837A MXPA02000837A (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected epitope regions.
AU59283/00A AU777550B2 (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected epitope regions
BR0012692-6A BR0012692A (en) 1999-07-22 2000-07-11 Protease conjugate, cleaning composition and personal treatment composition
EP00945317A EP1196547A2 (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected epitope regions
KR1020027000933A KR20020021396A (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected epitope regions
CA002379723A CA2379723A1 (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected epitope regions
JP2001512848A JP2003505069A (en) 1999-07-22 2000-07-11 Protease conjugates with sterically protected epitope regions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14497999P 1999-07-22 1999-07-22
US60/144,979 1999-07-22

Publications (2)

Publication Number Publication Date
WO2001007577A2 WO2001007577A2 (en) 2001-02-01
WO2001007577A3 true WO2001007577A3 (en) 2001-08-30

Family

ID=22511045

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/018854 WO2001007577A2 (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected epitope regions

Country Status (10)

Country Link
EP (1) EP1196547A2 (en)
JP (1) JP2003505069A (en)
KR (1) KR20020021396A (en)
CN (1) CN1372593A (en)
AU (1) AU777550B2 (en)
BR (1) BR0012692A (en)
CA (1) CA2379723A1 (en)
CZ (1) CZ2002211A3 (en)
MX (1) MXPA02000837A (en)
WO (1) WO2001007577A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835556B2 (en) 2002-12-31 2014-09-16 Nektar Therapeutics Hydrolytically stable maleimide-terminated polymers

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2509153C (en) 2002-12-31 2013-04-16 Nektar Therapeutics Al, Corporation Hydrolytically stable maleimide-terminated polymers
EP2105494B1 (en) 2008-03-25 2019-05-08 Diversey, Inc. A method of lubricating a conveyor belt
EP2105493B1 (en) 2008-03-25 2014-05-14 Diversey, Inc. Dry lubrication method employing oil-based lubricants
JP2012508031A (en) 2008-11-11 2012-04-05 ダニスコ・ユーエス・インク Protease containing one or more combination mutations
CA2743047A1 (en) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising serine protease variants

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0215662A2 (en) * 1985-09-13 1987-03-25 Hiroshi Maeda Anti-tumor protease preparations
EP0471125A1 (en) * 1989-02-20 1992-02-19 Kanebo, Ltd. Modified protease, method of producing the same and cosmetic compositions containing the modified protease
WO1992010755A1 (en) * 1990-12-05 1992-06-25 Novo Nordisk A/S Proteins with changed epitopes and methods for the production thereof
WO1997037007A1 (en) * 1996-03-29 1997-10-09 Genencor International, Inc. Enzyme multimer and process of producing same
WO1998023732A2 (en) * 1996-11-26 1998-06-04 Genencor International, Inc. Chemically modified enzymes
US5856451A (en) * 1994-12-07 1999-01-05 Novo Nordisk A/S Method for reducing respiratory allergenicity
WO1999000489A1 (en) * 1997-06-25 1999-01-07 Novo Nordisk A/S A modified polypeptide
WO1999037324A1 (en) * 1998-01-23 1999-07-29 Genencor International, Inc. Modified enzymes and their use for peptide synthesis
WO1999048918A1 (en) * 1998-03-26 1999-09-30 The Procter & Gamble Company Protease conjugates
WO2000028007A2 (en) * 1998-11-10 2000-05-18 Genencor International, Inc. Chemically modified mutant serine hydrolases
WO2000037658A2 (en) * 1998-12-21 2000-06-29 Genencor International, Inc. Chemically modified enzymes with multiple charged variants

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0215662A2 (en) * 1985-09-13 1987-03-25 Hiroshi Maeda Anti-tumor protease preparations
EP0471125A1 (en) * 1989-02-20 1992-02-19 Kanebo, Ltd. Modified protease, method of producing the same and cosmetic compositions containing the modified protease
WO1992010755A1 (en) * 1990-12-05 1992-06-25 Novo Nordisk A/S Proteins with changed epitopes and methods for the production thereof
US5856451A (en) * 1994-12-07 1999-01-05 Novo Nordisk A/S Method for reducing respiratory allergenicity
WO1997037007A1 (en) * 1996-03-29 1997-10-09 Genencor International, Inc. Enzyme multimer and process of producing same
WO1998023732A2 (en) * 1996-11-26 1998-06-04 Genencor International, Inc. Chemically modified enzymes
WO1999000489A1 (en) * 1997-06-25 1999-01-07 Novo Nordisk A/S A modified polypeptide
WO1999037324A1 (en) * 1998-01-23 1999-07-29 Genencor International, Inc. Modified enzymes and their use for peptide synthesis
WO1999048918A1 (en) * 1998-03-26 1999-09-30 The Procter & Gamble Company Protease conjugates
WO2000028007A2 (en) * 1998-11-10 2000-05-18 Genencor International, Inc. Chemically modified mutant serine hydrolases
WO2000037658A2 (en) * 1998-12-21 2000-06-29 Genencor International, Inc. Chemically modified enzymes with multiple charged variants

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GUNDLACH B R ET AL: "Determination of T cell epitopes with random peptide libraries", JOURNAL OF IMMUNOLOGICAL METHODS,NL,ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, vol. 192, no. 1, 10 June 1996 (1996-06-10), pages 149 - 155, XP004020829, ISSN: 0022-1759 *
SIEZEN ROLAND J ET AL: "Subtilases: The superfamily of subtilisin-like serine proteases.", PROTEIN SCIENCE, vol. 6, no. 3, 1997, pages 501 - 523, XP000990399, ISSN: 0961-8368 *
WALSH B AND HOWDEN M: "A method for detection of IgE binding sequences of allergens based on a modification of epitope mapping", JOURNAL OF IMMUNOLOGICAL METHODS,ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM,NL, vol. 121, 1989, pages 275 - 280-289, XP002112551, ISSN: 0022-1759 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835556B2 (en) 2002-12-31 2014-09-16 Nektar Therapeutics Hydrolytically stable maleimide-terminated polymers

Also Published As

Publication number Publication date
MXPA02000837A (en) 2002-07-30
CA2379723A1 (en) 2001-02-01
AU777550B2 (en) 2004-10-21
KR20020021396A (en) 2002-03-20
BR0012692A (en) 2002-04-09
AU5928300A (en) 2001-02-13
CN1372593A (en) 2002-10-02
WO2001007577A2 (en) 2001-02-01
EP1196547A2 (en) 2002-04-17
CZ2002211A3 (en) 2002-05-15
JP2003505069A (en) 2003-02-12

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