WO2001006008A3 - Multiplexed strand displacement for nucleic acid determinations - Google Patents
Multiplexed strand displacement for nucleic acid determinations Download PDFInfo
- Publication number
- WO2001006008A3 WO2001006008A3 PCT/US2000/018301 US0018301W WO0106008A3 WO 2001006008 A3 WO2001006008 A3 WO 2001006008A3 US 0018301 W US0018301 W US 0018301W WO 0106008 A3 WO0106008 A3 WO 0106008A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- determinations
- multiplexed
- oligonucleotides
- release
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 238000006073 displacement reaction Methods 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000000977 initiatory effect Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Multiplexed determinations of large numbers of events are achieved in an accurate and simple manner by using a combination of different oligonucleotides, which serve for both capture and release from a support and strand displacement oligonucleotides for sequential release of groups of oligonucleotides. Also included, as part of the oligonucleotide reagents may be identifiers, which serve to identify a particular characteristic. The method is illustrated using primers for initiation of chain extension joined to or serving as the capture/release sequence, where the extended primer has an identifier. After extending the primer, the extended primers are captured and independently released and the released extended primers assayed. The subject method finds application for nucleic acid sequence determinations, single nucleotide polymorphism determinations, identification of nucleic acid fragments, and the like.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002379749A CA2379749A1 (en) | 1999-07-16 | 2000-06-30 | Multiplexed strand displacement for nucleic acid determinations |
| JP2001511216A JP2003505036A (en) | 1999-07-16 | 2000-06-30 | Multiple strand displacement for nucleic acid determination |
| EP00948570A EP1200624A2 (en) | 1999-07-16 | 2000-06-30 | Multiplexed strand displacement for nucleic acid determinations |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35462999A | 1999-07-16 | 1999-07-16 | |
| US09/354,629 | 1999-07-16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2001006008A2 WO2001006008A2 (en) | 2001-01-25 |
| WO2001006008A3 true WO2001006008A3 (en) | 2001-07-12 |
Family
ID=23394232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2000/018301 WO2001006008A2 (en) | 1999-07-16 | 2000-06-30 | Multiplexed strand displacement for nucleic acid determinations |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1200624A2 (en) |
| JP (1) | JP2003505036A (en) |
| CA (1) | CA2379749A1 (en) |
| WO (1) | WO2001006008A2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001296668A1 (en) * | 2000-10-05 | 2002-04-15 | Aclara Biosciences, Inc. | Multiplexed differential displacement for nucleic acid determinations |
| US20060234253A1 (en) * | 2004-10-08 | 2006-10-19 | Sysmex Corporation | Method for detecting a target substance by using a nucleic acid probe |
| JP4839051B2 (en) * | 2004-10-08 | 2011-12-14 | シスメックス株式会社 | Method for detecting a test substance using a nucleic acid probe |
| AU2023390001A1 (en) * | 2022-12-07 | 2024-10-03 | Illumina, Inc. | Monoclonal clustering using double stranded dna size exclusion with patterned seeding |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1987003911A1 (en) * | 1985-12-17 | 1987-07-02 | Genetics Institute, Inc. | Displacement polynucleotide method and reagent complex |
| EP0269764A1 (en) * | 1986-12-01 | 1988-06-08 | Molecular Biosystems, Inc. | Method for increasing the sensitivity of nucleic acid hybridization assays |
| US4818680A (en) * | 1985-12-18 | 1989-04-04 | Mary Collins | Method and kit involving displacement and rehybridization of labeled polynucleotide |
| US5200314A (en) * | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| WO1998014610A2 (en) * | 1996-10-04 | 1998-04-09 | The Perkin-Elmer Corporation | Multiplex polynucleotide capture methods and compositions |
| EP0871774A1 (en) * | 1995-07-25 | 1998-10-21 | University Of Strathclyde | Detection of nucleic acids and nucleic acid units |
| WO1999024612A2 (en) * | 1997-11-06 | 1999-05-20 | Mosaic Technologies | Multiple sequential polynucleotide displacement reactions for signal amplification and processing |
-
2000
- 2000-06-30 WO PCT/US2000/018301 patent/WO2001006008A2/en not_active Application Discontinuation
- 2000-06-30 JP JP2001511216A patent/JP2003505036A/en not_active Withdrawn
- 2000-06-30 EP EP00948570A patent/EP1200624A2/en not_active Withdrawn
- 2000-06-30 CA CA002379749A patent/CA2379749A1/en not_active Abandoned
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1987003911A1 (en) * | 1985-12-17 | 1987-07-02 | Genetics Institute, Inc. | Displacement polynucleotide method and reagent complex |
| US4818680A (en) * | 1985-12-18 | 1989-04-04 | Mary Collins | Method and kit involving displacement and rehybridization of labeled polynucleotide |
| EP0269764A1 (en) * | 1986-12-01 | 1988-06-08 | Molecular Biosystems, Inc. | Method for increasing the sensitivity of nucleic acid hybridization assays |
| US5200314A (en) * | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| EP0871774A1 (en) * | 1995-07-25 | 1998-10-21 | University Of Strathclyde | Detection of nucleic acids and nucleic acid units |
| WO1998014610A2 (en) * | 1996-10-04 | 1998-04-09 | The Perkin-Elmer Corporation | Multiplex polynucleotide capture methods and compositions |
| WO1999024612A2 (en) * | 1997-11-06 | 1999-05-20 | Mosaic Technologies | Multiple sequential polynucleotide displacement reactions for signal amplification and processing |
Non-Patent Citations (1)
| Title |
|---|
| WOLCOTT M J: "ADVANCES IN NUCLEIC ACID-BASED DETECTION METHODS", CLINICAL MICROBIOLOGY REVIEWS,US,WASHINGTON, DC, vol. 5, no. 4, 1 October 1992 (1992-10-01), pages 370 - 386, XP000606216, ISSN: 0893-8512 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1200624A2 (en) | 2002-05-02 |
| WO2001006008A2 (en) | 2001-01-25 |
| CA2379749A1 (en) | 2001-01-25 |
| JP2003505036A (en) | 2003-02-12 |
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