+

WO2001004360A3 - Retroviral recombination assays and uses thereof - Google Patents

Retroviral recombination assays and uses thereof Download PDF

Info

Publication number
WO2001004360A3
WO2001004360A3 PCT/US2000/018597 US0018597W WO0104360A3 WO 2001004360 A3 WO2001004360 A3 WO 2001004360A3 US 0018597 W US0018597 W US 0018597W WO 0104360 A3 WO0104360 A3 WO 0104360A3
Authority
WO
WIPO (PCT)
Prior art keywords
retroviral
transfer
retroviral recombination
recombination assays
assays
Prior art date
Application number
PCT/US2000/018597
Other languages
French (fr)
Other versions
WO2001004360A9 (en
WO2001004360A2 (en
Inventor
John C Kappes
Xiaoyun Wu
John Wakeield
Original Assignee
Uab Research Foundation
Tranzyme Inc
John C Kappes
Xiaoyun Wu
John Wakeield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uab Research Foundation, Tranzyme Inc, John C Kappes, Xiaoyun Wu, John Wakeield filed Critical Uab Research Foundation
Priority to CA002379207A priority Critical patent/CA2379207A1/en
Priority to AU60766/00A priority patent/AU6076600A/en
Priority to EP00947102A priority patent/EP1226282A2/en
Publication of WO2001004360A2 publication Critical patent/WO2001004360A2/en
Publication of WO2001004360A3 publication Critical patent/WO2001004360A3/en
Publication of WO2001004360A9 publication Critical patent/WO2001004360A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
    • C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/48—Vector systems having a special element relevant for transcription regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods, systems, and cells are described that can be used to sensitively monitor for retroviral recombination. Using the invention, recombinant proviruses can be detected to provide an evaluation of the risk of creating a replication competent retrovirus (RCR) in vivo. The invention can further be used to improve on existing procedures in the art, including Tat transfer, Gag transfer, and marker rescue and mobilization assays.
PCT/US2000/018597 1999-07-09 2000-07-06 Retroviral recombination assays and uses thereof WO2001004360A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002379207A CA2379207A1 (en) 1999-07-09 2000-07-06 Retroviral recombination assays and uses thereof
AU60766/00A AU6076600A (en) 1999-07-09 2000-07-06 Retroviral recombination assays and uses thereof
EP00947102A EP1226282A2 (en) 1999-07-09 2000-07-06 Retroviral recombination assays and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US14301599P 1999-07-09 1999-07-09
US60/143,015 1999-07-09
US16462699P 1999-11-10 1999-11-10
US60/164,626 1999-11-10

Publications (3)

Publication Number Publication Date
WO2001004360A2 WO2001004360A2 (en) 2001-01-18
WO2001004360A3 true WO2001004360A3 (en) 2002-05-30
WO2001004360A9 WO2001004360A9 (en) 2002-09-12

Family

ID=26840604

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/018597 WO2001004360A2 (en) 1999-07-09 2000-07-06 Retroviral recombination assays and uses thereof

Country Status (4)

Country Link
EP (1) EP1226282A2 (en)
AU (1) AU6076600A (en)
CA (1) CA2379207A1 (en)
WO (1) WO2001004360A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10167509B2 (en) 2011-02-09 2019-01-01 Bio-Rad Laboratories, Inc. Analysis of nucleic acids

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6656706B2 (en) * 1999-12-23 2003-12-02 The United States Of America As Represented By The Department Of Health And Human Services Molecular clones with mutated HIV gag/pol, SIV gag and SIV env genes
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
JP2004533827A (en) * 2001-05-17 2004-11-11 アールフス・ユニバーシティー Improved vector for gene therapy
US9944998B2 (en) 2013-07-25 2018-04-17 Bio-Rad Laboratories, Inc. Genetic assays
US11421287B2 (en) 2016-07-29 2022-08-23 Juno Therapeutics, Inc. Methods for assessing the presence or absence of replication competent virus
IL296319A (en) * 2020-03-09 2022-11-01 Janssen Biotech Inc Compositions and Methods for Quantifying Recombinant Vector Nucleic Acid Incorporation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0463570A1 (en) * 1990-06-22 1992-01-02 R.O.B.I.T. RESEARCH AND DEVELOPMENT COMPANY LTD., c/o EFRATI, GALILI & CO. Detection of HIV infection by bioluminescence
WO1997008330A1 (en) * 1995-08-23 1997-03-06 Cancer Research Campaign Technology Limited Expression systems

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0463570A1 (en) * 1990-06-22 1992-01-02 R.O.B.I.T. RESEARCH AND DEVELOPMENT COMPANY LTD., c/o EFRATI, GALILI & CO. Detection of HIV infection by bioluminescence
WO1997008330A1 (en) * 1995-08-23 1997-03-06 Cancer Research Campaign Technology Limited Expression systems

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHANG L-J ET AL: "Efficacy and safety analyses of a recombinant human immunodeficiency virus type 1 derived vector system.", GENE THERAPY, vol. 6, no. 5, May 1999 (1999-05-01), pages 715 - 728, XP000872478 *
KAFRI T ET AL: "A packaging cell line for lentivirus vectors.", JOURNAL OF VIROLOGY, vol. 73, no. 1, January 1999 (1999-01-01), pages 576 - 584, XP002921432 *
NALDINI L ET AL: "In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector.", SCIENCE, vol. 272, no. 5259, 12 April 1996 (1996-04-12), pages 263 - 267, XP000583652 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10167509B2 (en) 2011-02-09 2019-01-01 Bio-Rad Laboratories, Inc. Analysis of nucleic acids

Also Published As

Publication number Publication date
WO2001004360A9 (en) 2002-09-12
CA2379207A1 (en) 2001-01-18
AU6076600A (en) 2001-01-30
EP1226282A2 (en) 2002-07-31
WO2001004360A2 (en) 2001-01-18

Similar Documents

Publication Publication Date Title
NO20001508D0 (en) Fusion proteins comprising HIV-1 TAT and / or NEF proteins
DE3772087D1 (en) QUICK COUPLING FOR PIPES, PILE AND SIMILAR.
AU2002221771A1 (en) Cation-conducting or proton-conducting ceramic membrane based on a hydroxysilylic acid, method for the production thereof and use of the same
NO913538L (en) ACID RESISTANT CONCRETE STANDS, SPECIAL SULFUR CONCRETE BEARS, AND PROCEDURE FOR MANUFACTURING.
WO2001044286A3 (en) Five-helix protein
EP0933824A3 (en) Separator for gel electrolyte battery
ATE445411T1 (en) MVA-EXPRESSING MODIFIED HIV ENVELOPE, GAG AND POL GENES
NO862545D0 (en) WATER-BASED POLYMER MATERIALS AND PROCEDURES FOR MANUFACTURING, AND USE THEREOF.
WO2001004360A3 (en) Retroviral recombination assays and uses thereof
NO165087C (en) ULTRA AUDIO DEVICE AND PROCEDURE FOR AA DETECTED ERRORS OR CRACKS IN A WORK PIECE.
NO862975L (en) QUATERNALLY OXALKYLED POLYCONDENSED PROCEDURES FOR THE PREPARATION OF THEIR PREPARATION AND THEIR USE.
DE3882349D1 (en) WET-RAILED TRAIN, USED AS A COATING MATERIAL.
IT1207271B (en) AMMINOGLICOSIDES, PROCEDURE FOR THEIR PREPARATION AND USE.
DK0975783T3 (en) Retroviral vectors, their method of preparation and their use for gene transfer to CD4 positive cells
NO900943D0 (en) COPOLYMERS, THE PROCEDURE FOR THEIR PREPARATION AND USE.
PT880594E (en) RETROVIRAL VECTOR AND ITS USE IN GENE THERAPY
DE68904552D1 (en) SUSPENSED MEMBRANE JOINT.
NO893226L (en) PROCEDURE FOR AA PREVENTING MICROBIOLOGICAL PROVISIONS IN PAPER MAKING EQUIPMENT.
AU7289587A (en) Immunosuppressive factor from human immunodeficiency virus (hiv) or hiv infected cells or cell lines, its use, corresponding antibodies and their use
NO894494D0 (en) DIFFICULT ART RESPONSE DISPERSIONS, AND THE PROCEDURE FOR MAKING THEM, AND USING THE DISPERSIONS.
DE69002873D1 (en) Self-centering chuck with balancing jaws.
AUPP700798A0 (en) Recombinant viral constructs and methods relating thereto
JPS54154263A (en) Multi-factor detection load cell
AU622625B2 (en) Aids diagnosis test based on the detection of a diversity of the isotypic response, vaccines and serotherapies
IT1311827B1 (en) SELF-CENTERING TILTING FORK OF THE FORK, PARTICULARLY FOR MEASURING ON FOUR POINTS OF THE

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2379207

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 516643

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 2000947102

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 60766/00

Country of ref document: AU

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWP Wipo information: published in national office

Ref document number: 2000947102

Country of ref document: EP

AK Designated states

Kind code of ref document: C2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 1/30-30/30, DRAWINGS, REPLACED BY NEW PAGES 1/29-29/29; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2000947102

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载