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WO2001098747A3 - Fixation d'agoniste/antagoniste a des recepteurs couples a la proteine g - Google Patents

Fixation d'agoniste/antagoniste a des recepteurs couples a la proteine g Download PDF

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Publication number
WO2001098747A3
WO2001098747A3 PCT/US2001/019871 US0119871W WO0198747A3 WO 2001098747 A3 WO2001098747 A3 WO 2001098747A3 US 0119871 W US0119871 W US 0119871W WO 0198747 A3 WO0198747 A3 WO 0198747A3
Authority
WO
WIPO (PCT)
Prior art keywords
binding
agonist
protein
coupled receptors
molecular
Prior art date
Application number
PCT/US2001/019871
Other languages
English (en)
Other versions
WO2001098747A2 (fr
Inventor
Zdzislaw Salamon
Scott Cowell
Victor J Hruby
Gordon Tollin
Original Assignee
Univ Arizona
Zdzislaw Salamon
Scott Cowell
Victor J Hruby
Gordon Tollin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Arizona, Zdzislaw Salamon, Scott Cowell, Victor J Hruby, Gordon Tollin filed Critical Univ Arizona
Priority to JP2002504459A priority Critical patent/JP2004507716A/ja
Priority to AU2001270046A priority patent/AU2001270046A1/en
Priority to EP01948579A priority patent/EP1295120A2/fr
Priority to CA002413081A priority patent/CA2413081A1/fr
Publication of WO2001098747A2 publication Critical patent/WO2001098747A2/fr
Publication of WO2001098747A3 publication Critical patent/WO2001098747A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Procédé servant à caractériser les propriétés biophysiques de récepteurs couplés à la protéine G en réponse à leur fixation par des ligands. On a effectué des investigations sur le récepteur de δ-opioïdes cloné humain immobilisé dans une bicouche lipidique supportée par un solide au moyen d'un procédé mettant en application la spectroscopie par résonance de guides d'ondes au plasmon couplés (CPWR). L'invention présente un procédé extrêmement sensible permettant de contrôler directement les modifications de densité de masse, de conformation et d'orientation moléculaire apparaissant dans des couches minces anisotropes et de déterminer directement des constantes de fixation. Bien que la fixation à la fois de l'agoniste et de l'antagoniste au récepteur provoque des augmentations de l'ordonnancement moléculaire à l'intérieur de la membrane protéolipidique, seule la fixation de l'agoniste induit une augmentation de l'épaisseur et de la densité du regroupement moléculaire de la membrane (10). Ceci constitue un procédé servant à effectuer une distinction entre la fixation de l'agoniste et celle de l'antagoniste.
PCT/US2001/019871 2000-06-22 2001-06-21 Fixation d'agoniste/antagoniste a des recepteurs couples a la proteine g WO2001098747A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2002504459A JP2004507716A (ja) 2000-06-22 2001-06-21 Ｇ蛋白質−結合レセプタのアゴニスト及びアンタゴニストのリガンドを区別する方法
AU2001270046A AU2001270046A1 (en) 2000-06-22 2001-06-21 Agonist versus antagonist binding to g protein-coupled receptors
EP01948579A EP1295120A2 (fr) 2000-06-22 2001-06-21 Fixation d'agoniste/antagoniste a des recepteurs couples a la proteine g
CA002413081A CA2413081A1 (fr) 2000-06-22 2001-06-21 Fixation d'agoniste/antagoniste a des recepteurs couples a la proteine g

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21357500P 2000-06-22 2000-06-22
US60/213,575 2000-06-22

Publications (2)

Publication Number Publication Date
WO2001098747A2 WO2001098747A2 (fr) 2001-12-27
WO2001098747A3 true WO2001098747A3 (fr) 2002-04-11

Family

ID=22795635

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/019871 WO2001098747A2 (fr) 2000-06-22 2001-06-21 Fixation d'agoniste/antagoniste a des recepteurs couples a la proteine g

Country Status (5)

Country Link
EP (1) EP1295120A2 (fr)
JP (1) JP2004507716A (fr)
AU (1) AU2001270046A1 (fr)
CA (1) CA2413081A1 (fr)
WO (1) WO2001098747A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030006192A1 (en) * 1998-02-23 2003-01-09 Irving Wainer Immobilized G-protein coupled receptors on liquid chromatography stationary phases and use thereof for on-line screening
US7678539B2 (en) * 2000-08-10 2010-03-16 Corning Incorporated Arrays of biological membranes and methods and use thereof
US20160334395A1 (en) * 2014-01-21 2016-11-17 Nanyang Technological University Hydration layer/lipid bilayer structures

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5991448A (en) * 1994-10-28 1999-11-23 Oki Electric Industry Co., Ltd. Image encoding and decoding method and apparatus using edge synthesis and inverse wavelet transform

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5991448A (en) * 1994-10-28 1999-11-23 Oki Electric Industry Co., Ltd. Image encoding and decoding method and apparatus using edge synthesis and inverse wavelet transform

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BEIRI ET AL.: "Micropatterned immobilization of G protein-coupled receptor: and direct detection of G protein activation", NAT. BIOTECHNOL., vol. 17, November 1999 (1999-11-01), pages 1105 - 1108, XP002183971 *
SALAMON ET AL.: "Coupled plasmon-waveguide resonators: A new spectroscopis tool for probing proteolipid film structure and properties", BIOPHYS. J., vol. 75, October 1998 (1998-10-01), pages 1874 - 1885, XP002906545 *
SLEPAK V.Z.: "Application of surface plasmon resonance for analysis of protein-protein interactions in the G protein-mediated signal transduction pathway", J. MOL. RECOGNIT., vol. 13, January 2000 (2000-01-01), pages 20 - 26, XP002906134 *

Also Published As

Publication number Publication date
CA2413081A1 (fr) 2001-12-27
WO2001098747A2 (fr) 2001-12-27
JP2004507716A (ja) 2004-03-11
EP1295120A2 (fr) 2003-03-26
AU2001270046A1 (en) 2002-01-02

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