+

WO2001092477A3 - Modele de peau tridimensionnel - Google Patents

Modele de peau tridimensionnel Download PDF

Info

Publication number
WO2001092477A3
WO2001092477A3 PCT/EP2001/006074 EP0106074W WO0192477A3 WO 2001092477 A3 WO2001092477 A3 WO 2001092477A3 EP 0106074 W EP0106074 W EP 0106074W WO 0192477 A3 WO0192477 A3 WO 0192477A3
Authority
WO
WIPO (PCT)
Prior art keywords
vitro
methods
equivalent
dimensional
skin model
Prior art date
Application number
PCT/EP2001/006074
Other languages
German (de)
English (en)
Other versions
WO2001092477A2 (fr
Inventor
Michaela Noll
Thomas Graeve
Original Assignee
Fraunhofer Ges Forschung
Michaela Noll
Thomas Graeve
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10026789A external-priority patent/DE10026789B4/de
Priority claimed from DE10062623A external-priority patent/DE10062623B4/de
Priority to JP2002500671A priority Critical patent/JP4751005B2/ja
Priority to EP01953961A priority patent/EP1290145B1/fr
Priority to DE50107916T priority patent/DE50107916D1/de
Priority to CA2410956A priority patent/CA2410956C/fr
Application filed by Fraunhofer Ges Forschung, Michaela Noll, Thomas Graeve filed Critical Fraunhofer Ges Forschung
Priority to US10/296,967 priority patent/US7553664B2/en
Priority to AT01953961T priority patent/ATE308610T1/de
Priority to AU76348/01A priority patent/AU7634801A/en
Publication of WO2001092477A2 publication Critical patent/WO2001092477A2/fr
Publication of WO2001092477A3 publication Critical patent/WO2001092477A3/fr
Priority to US12/484,810 priority patent/US8222031B2/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3813—Epithelial cells, e.g. keratinocytes, urothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A61L27/3852—Cartilage, e.g. meniscus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues
    • C12N2503/06—Screening or testing on artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Transplantation (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Dermatology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Rheumatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Vascular Medicine (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)

Abstract

L'invention concerne un procédé de culture de fibroblastes dermiques, un procédé de préparation d'équivalents de derme in vitro, un procédé de préparation d'équivalents de peau in vitro tridimensionnels, un équivalent de derme in vitro, un équivalent de peau in vitro tridimensionnel, ainsi qu'un procédé de détermination de l'action d'une substance chimique ou d'un agent sur des cellules de peau humaines, ledit procédé utilisant l'équivalent de derme in vitro et/ou l'équivalent de peau in vitro.
PCT/EP2001/006074 2000-05-31 2001-05-29 Modele de peau tridimensionnel WO2001092477A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
AU76348/01A AU7634801A (en) 2000-05-31 2001-05-29 Three-dimensional skin model
AT01953961T ATE308610T1 (de) 2000-05-31 2001-05-29 Dreidimensionales hautmodell
EP01953961A EP1290145B1 (fr) 2000-05-31 2001-05-29 Modele de peau tridimensionnel
DE50107916T DE50107916D1 (de) 2000-05-31 2001-05-29 Dreidimensionales hautmodell
CA2410956A CA2410956C (fr) 2000-05-31 2001-05-29 Modele de peau tridimensionnel
JP2002500671A JP4751005B2 (ja) 2000-05-31 2001-05-29 三次元皮膚モデル
US10/296,967 US7553664B2 (en) 2000-05-31 2001-05-29 Three-dimensional skin model
US12/484,810 US8222031B2 (en) 2000-05-31 2009-06-15 Three-dimensional skin model

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10026789.O 2000-05-31
DE10026789A DE10026789B4 (de) 2000-05-31 2000-05-31 Knorpelersatz und Verfahren zu dessen Herstellung
DE10062623.8 2000-12-15
DE10062623A DE10062623B4 (de) 2000-05-31 2000-12-15 Dreidimensionales Hautmodell

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10296967 A-371-Of-International 2001-05-29
US12/484,810 Division US8222031B2 (en) 2000-05-31 2009-06-15 Three-dimensional skin model

Publications (2)

Publication Number Publication Date
WO2001092477A2 WO2001092477A2 (fr) 2001-12-06
WO2001092477A3 true WO2001092477A3 (fr) 2002-05-10

Family

ID=26005890

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/006074 WO2001092477A2 (fr) 2000-05-31 2001-05-29 Modele de peau tridimensionnel

Country Status (10)

Country Link
US (2) US7553664B2 (fr)
EP (1) EP1290145B1 (fr)
JP (1) JP4751005B2 (fr)
AT (1) ATE308610T1 (fr)
AU (1) AU7634801A (fr)
CA (1) CA2410956C (fr)
DE (1) DE50107916D1 (fr)
DK (1) DK1290145T3 (fr)
ES (1) ES2252259T3 (fr)
WO (1) WO2001092477A2 (fr)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7741116B2 (en) * 2002-03-06 2010-06-22 University Of Cincinnati Surgical device for skin therapy or testing
DE102004039537A1 (de) * 2004-08-13 2006-02-23 Phenion Gmbh & Co. Kg Vernetzte Kollagenmatrix zur Herstellung eines Hautäquivalentes
US7247423B2 (en) * 2004-09-10 2007-07-24 Synergy Biosystems Ltd Method to measure the effect of topically applied agents using skin maintained in organ culture
DE102005028899B4 (de) * 2005-06-17 2007-09-20 Gkss-Forschungszentrum Geesthacht Gmbh Hautäquivalent und Verfahren zur Erzeugung desselben
US20070249044A1 (en) * 2006-01-30 2007-10-25 University Of Illinois At Chicago Microstructures in three dimensional gel suspensions for growth of cells
US8591933B2 (en) * 2007-07-18 2013-11-26 The Board Of Trustees Of The University Of Illinois Temporal release of growth factors from 3D micro rod scaffolds for tissue regeneration
KR101739330B1 (ko) * 2010-10-29 2017-05-24 (주)아모레퍼시픽 진피 모사체를 이용한 피부 탄력 증진력 평가법
DE102011121556B4 (de) 2011-12-20 2015-12-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. In vitro 3D-Reporter-Hautmodell
US9878071B2 (en) * 2013-10-16 2018-01-30 Purdue Research Foundation Collagen compositions and methods of use
EP3137594B1 (fr) 2014-04-30 2018-03-28 Fondazione Istituto Italiano di Tecnologia Procédé de production d'un tissu totalement endogène produit par génie biologique et tissu ainsi obtenu
DE102014214944A1 (de) 2014-07-30 2016-02-04 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtung zur Erzeugung reproduzierbarer Schnitte und/oder Ausnehmung in zumindest einer oberflächenelastischen Probe
EP3185922B1 (fr) 2014-08-27 2025-04-02 Purdue Research Foundation Systèmes d'administration thérapeutique à base de collagène
EP3072535B1 (fr) * 2015-03-26 2017-03-01 Université de Bordeaux Procédé de reconstruction de peau
US11919941B2 (en) 2015-04-21 2024-03-05 Purdue Research Foundation Cell-collagen-silica composites and methods of making and using the same
EP3115766A1 (fr) 2015-07-10 2017-01-11 3Scan Inc. Multiplexage spatial de colorations histologiques
KR20170055791A (ko) 2015-11-12 2017-05-22 한국과학기술연구원 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법
US12280176B2 (en) 2017-01-31 2025-04-22 Geniphys, Inc. Methods and compositions for matrix preparation
WO2018200750A1 (fr) 2017-04-25 2018-11-01 Purdue Research Foundation Muscle artificiel tridimensionnel (3d) de restauration tissulaire
US11105795B2 (en) 2018-01-22 2021-08-31 ClearIt, LLC Methods and compositions for simulation of the dermal compartment
FR3083246B1 (fr) * 2018-06-29 2023-12-08 Oreal Modeles cellulaires et tissulaires comprenant des fibroblastes de la jonction dermo-hypodermique et ses applications
US11911090B2 (en) 2018-12-19 2024-02-27 Clear Intradermal Technologies, Inc. Systems and methods for tattoo removal using an applied electric field
CN109602958B (zh) * 2019-01-11 2021-04-06 湖北中部医疗科技有限公司 一种人工皮肤及其制备方法
MX2023008555A (es) 2021-01-22 2023-08-07 Prolacta Bioscience Inc Formulaciones topicas de leche humana.
DE102021105932B4 (de) 2021-03-11 2023-02-16 Leica Biosystems Nussloch Gmbh Verfahren zur Gewebeprozessierung und Gewebeprozessor
CN113667632B (zh) * 2021-09-14 2023-10-17 上海艾济生物科技有限公司 细胞培养基添加剂、细胞培养基及细胞体外扩增的方法
GB202201229D0 (en) * 2022-01-31 2022-03-16 Alcyomics Ltd An innervated cellular composite
LU502391B1 (en) 2022-06-28 2024-01-09 Univerza V Mariboru A complex in vitro model of human skin, a process for preparation and use thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016010A1 (fr) * 1990-04-24 1991-10-31 Mark Eisenberg Equivalents composites de peau vivante
US5478739A (en) * 1992-10-23 1995-12-26 Advanced Tissue Sciences, Inc. Three-dimensional stromal cell and tissue culture system
WO1997041208A1 (fr) * 1996-04-26 1997-11-06 Case Western Reserve University Regeneration de la peau au moyen des cellules souches mesenchymateuses
EP0851227A1 (fr) * 1996-12-24 1998-07-01 L'oreal Procédé d'évaluation des dommages induits dans la peau par UV-A
US5945101A (en) * 1993-03-17 1999-08-31 Berg; Richard A. Skin model system

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6133593B2 (fr) 1978-12-26 1986-08-02 Massachusetts Inst Technology
US4485096A (en) * 1982-02-26 1984-11-27 Massachusetts Institute Of Technology Tissue-equivalent and method for preparation thereof
US4604346A (en) 1984-10-09 1986-08-05 Massachusetts Institute Of Technology Skin-equivalent prepared by the use of punch biopsy
US5902741A (en) 1986-04-18 1999-05-11 Advanced Tissue Sciences, Inc. Three-dimensional cartilage cultures
US4963489A (en) 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
FR2612939B1 (fr) 1987-03-26 1989-06-23 Cird Equivalent de peau
IL95429A (en) 1989-09-15 1997-09-30 Organogenesis Living tissue equivalents comprising hydrated collagen lattice and a collagen gel and their production
US5368224A (en) * 1992-10-23 1994-11-29 Nellcor Incorporated Method for reducing ambient noise effects in electronic monitoring instruments
WO1995010600A1 (fr) 1993-10-08 1995-04-20 Martin Rosdy Test de bronzage epidermique humain in vitro
US5713367A (en) * 1994-01-26 1998-02-03 Cambridge Heart, Inc. Measuring and assessing cardiac electrical stability
US5658190A (en) 1995-12-15 1997-08-19 Micron Technology, Inc. Apparatus for separating wafers from polishing pads used in chemical-mechanical planarization of semiconductor wafers
FR2743817B1 (fr) 1996-01-23 1998-03-13 Oreal Equivalent de peau comprenant des cellules de langerhans
US5886788A (en) * 1996-02-09 1999-03-23 Sony Corporation Apparatus and method for detecting a posture
US5919702A (en) 1996-10-23 1999-07-06 Advanced Tissue Science, Inc. Production of cartilage tissue using cells isolated from Wharton's jelly
DE19721661A1 (de) 1997-05-23 1998-11-26 Zimmer Markus Knochen- und Knorpel Ersatzstrukturen
EP0989867B1 (fr) 1997-06-27 2002-04-24 BADER, Augustinus Transplant biosynthetique et son procede de production
DE69714035T2 (de) 1997-08-14 2003-03-06 Sulzer Innotec Ag, Winterthur Zusammensetzung und Vorrichtung zur Reparatur von Knorpelgewebe in vivo bestehend aus Nanokapseln mit osteoinduktiven und/oder chondroinduktiven Faktoren
US7499750B2 (en) * 2003-04-11 2009-03-03 Cardiac Pacemakers, Inc. Noise canceling cardiac electrodes
US7559901B2 (en) * 2004-07-28 2009-07-14 Cardiac Pacemakers, Inc. Determining a patient's posture from mechanical vibrations of the heart
US7403813B1 (en) * 2004-11-24 2008-07-22 Pacesetter, Inc. Systems and methods for detection of VT and VF from remote sensing electrodes
US7662104B2 (en) * 2005-01-18 2010-02-16 Cardiac Pacemakers, Inc. Method for correction of posture dependence on heart sounds

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016010A1 (fr) * 1990-04-24 1991-10-31 Mark Eisenberg Equivalents composites de peau vivante
US5478739A (en) * 1992-10-23 1995-12-26 Advanced Tissue Sciences, Inc. Three-dimensional stromal cell and tissue culture system
US5945101A (en) * 1993-03-17 1999-08-31 Berg; Richard A. Skin model system
WO1997041208A1 (fr) * 1996-04-26 1997-11-06 Case Western Reserve University Regeneration de la peau au moyen des cellules souches mesenchymateuses
EP0851227A1 (fr) * 1996-12-24 1998-07-01 L'oreal Procédé d'évaluation des dommages induits dans la peau par UV-A

Also Published As

Publication number Publication date
DK1290145T3 (da) 2006-03-13
EP1290145B1 (fr) 2005-11-02
WO2001092477A2 (fr) 2001-12-06
AU7634801A (en) 2001-12-11
CA2410956A1 (fr) 2001-12-06
US20040018149A1 (en) 2004-01-29
ATE308610T1 (de) 2005-11-15
DE50107916D1 (de) 2005-12-08
US8222031B2 (en) 2012-07-17
US7553664B2 (en) 2009-06-30
JP2004505614A (ja) 2004-02-26
ES2252259T3 (es) 2006-05-16
JP4751005B2 (ja) 2011-08-17
CA2410956C (fr) 2012-12-04
EP1290145A2 (fr) 2003-03-12
US20090298042A1 (en) 2009-12-03

Similar Documents

Publication Publication Date Title
WO2001092477A3 (fr) Modele de peau tridimensionnel
AU2002356861A1 (en) Fibrin membrane and methods for its preparation- application to artificial skin
WO2003008647A3 (fr) Evaluation sanguine de maux
EP2977043A3 (fr) Mousse pharmaceutique pénétrante
AU2002230642A1 (en) Proton generating catheters and methods for their use
ES2243801T3 (es) Equivalente de piel/cabello con papilas reconstruidas.
ITRM960799A0 (it) Uso di batteri lattici per accrescere il livello delle ceramidi della pelle e della mucosa, e composizioni dermatologiche e cosmetiche atte
EG23321A (en) Artificial dermis and method of preparation.
AU2001240940A1 (en) Cosmetic and dermatological composition for psoriatic skin, scalp treatment and care
WO2001032840A3 (fr) Transplantation de cheveux
MXPA03010429A (es) Producto topico con indicador visual.
WO2003097072A9 (fr) Composition de traitement de chloasma et composition de traitement de matite
AU2000276877A1 (en) Process for producing gel sheet for application to living body, gel sheet for application to living body obtained by the production process, and method of skin care with the same
EP1172084A3 (fr) Composition cosmétique et dermatologique pour éliminer du sébum
AU2001247880A1 (en) Methods to prepare and use epidermal stem cells
WO2003037288A3 (fr) Composition cosmetique mimant le sebum, et utilisations
WO2001062208A3 (fr) Agents cosmetiques topiques contenant des 2-hydrazino-1,3-heteroazols
EP0993825A3 (fr) Composition contenant de l'urée et ses utilisations dans le domaine cosmétique et/ou dermatologique
WO2006018454A3 (fr) Preparation cosmetique contenant de l'acide hyaluronique
AU2002224471A1 (en) In vivo stimulation of angiogenic activity
CA2280185A1 (fr) Nouveaux derives de l'acide salicylique et leur utilisation dans les compositions cosmetiques ou dermatologiques
AU2002352566A1 (en) Use of corrinoids in the treatment of skin diseases
AU7944700A (en) Bio-artificial substrate for the production of animal and, in particular, human tissues and organs
WO2002030443A3 (fr) Regulation de la cicatrisation de la peau humaine
AU3791201A (en) Method of forming micropores in skin

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2001953961

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2410956

Country of ref document: CA

WWP Wipo information: published in national office

Ref document number: 2001953961

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10296967

Country of ref document: US

WWG Wipo information: grant in national office

Ref document number: 2001953961

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载