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WO2001088114A3 - Gene expression system - Google Patents

Gene expression system Download PDF

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Publication number
WO2001088114A3
WO2001088114A3 PCT/EP2001/005794 EP0105794W WO0188114A3 WO 2001088114 A3 WO2001088114 A3 WO 2001088114A3 EP 0105794 W EP0105794 W EP 0105794W WO 0188114 A3 WO0188114 A3 WO 0188114A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression
gene expression
expression system
recombinant polypeptides
vectors
Prior art date
Application number
PCT/EP2001/005794
Other languages
French (fr)
Other versions
WO2001088114A2 (en
Inventor
Geert Plaetinck
Jean-Pierre Renard
Katherine Mortier
Thierry Bogaert
Original Assignee
Devgen Nv
Geert Plaetinck
Jean-Pierre Renard
Katherine Mortier
Thierry Bogaert
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Devgen Nv, Geert Plaetinck, Jean-Pierre Renard, Katherine Mortier, Thierry Bogaert filed Critical Devgen Nv
Priority to AU75671/01A priority Critical patent/AU7567101A/en
Publication of WO2001088114A2 publication Critical patent/WO2001088114A2/en
Publication of WO2001088114A3 publication Critical patent/WO2001088114A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/75—Vector systems having a special element relevant for transcription from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
    • C12N2840/445—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor for trans-splicing, e.g. polypyrimidine tract, branch point splicing

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

There are provided DNA constructs, including replicable cloning vectors and expression vectors, comprising a bacteriophage promoter operably linked to an outron sequence. The expression vectors provided by the invention are useful in the expression of recombinant polypeptides in host cells or organisms and are particularly useful in expression of recombinant polypeptides in nematode worms such as C. elegans.
PCT/EP2001/005794 2000-05-19 2001-05-18 Gene expression system WO2001088114A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU75671/01A AU7567101A (en) 2000-05-19 2001-05-18 Gene expression system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0012231A GB2362383B (en) 2000-05-19 2000-05-19 Gene expression system
GB0012231.7 2000-05-19

Publications (2)

Publication Number Publication Date
WO2001088114A2 WO2001088114A2 (en) 2001-11-22
WO2001088114A3 true WO2001088114A3 (en) 2002-06-20

Family

ID=9891998

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/005794 WO2001088114A2 (en) 2000-05-19 2001-05-18 Gene expression system

Country Status (3)

Country Link
AU (1) AU7567101A (en)
GB (1) GB2362383B (en)
WO (1) WO2001088114A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7538095B2 (en) 1997-12-23 2009-05-26 The Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
US7888325B2 (en) 1999-01-28 2011-02-15 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
US9051566B2 (en) 2001-01-31 2015-06-09 Alnylam Pharmaceuticals, Inc. Post-transcriptional gene silencing using expressed double stranded RNA

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ295108B6 (en) 1998-03-20 2005-05-18 Benitec Australia Ltd Synthetic gene comprising dispersed or foreign deoxyribonucleic molecule and a gene construct containing such a synthetic gene
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991000905A1 (en) * 1989-07-07 1991-01-24 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Rapid, versatile and simple system for expressing genes in eukaryotic cells
WO1997022250A1 (en) * 1995-12-15 1997-06-26 Intronn Llc Therapeutic molecules generated by trans-splicing
WO1999037770A1 (en) * 1998-01-23 1999-07-29 Cold Spring Harbor Laboratory Phagocytosis genes and uses thereof
WO2000001846A2 (en) * 1998-07-03 2000-01-13 Devgen N.V. Characterisation of gene function using double stranded rna inhibition
WO2000009734A2 (en) * 1998-08-13 2000-02-24 Intronn Holdings, Llc Methods and compositions for use in spliceosome mediated rna trans-splicing
WO2001032896A1 (en) * 1999-11-05 2001-05-10 Jena Bioscience Gmbh Protein expression systems for non-pathogenic <i>kinetoplastidae</i>

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991000905A1 (en) * 1989-07-07 1991-01-24 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Rapid, versatile and simple system for expressing genes in eukaryotic cells
WO1997022250A1 (en) * 1995-12-15 1997-06-26 Intronn Llc Therapeutic molecules generated by trans-splicing
WO1999037770A1 (en) * 1998-01-23 1999-07-29 Cold Spring Harbor Laboratory Phagocytosis genes and uses thereof
WO2000001846A2 (en) * 1998-07-03 2000-01-13 Devgen N.V. Characterisation of gene function using double stranded rna inhibition
WO2000009734A2 (en) * 1998-08-13 2000-02-24 Intronn Holdings, Llc Methods and compositions for use in spliceosome mediated rna trans-splicing
WO2001032896A1 (en) * 1999-11-05 2001-05-10 Jena Bioscience Gmbh Protein expression systems for non-pathogenic <i>kinetoplastidae</i>

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CONRAD R ET AL: "Insertion of part of an intron into the 5' untranslated region of a Caenorhabditis elegans gene converts it into a trans-spliced gene", MOL CELL BIOL, vol. 11, no. 4, April 1991 (1991-04-01), pages 1921 - 1926, XP001073410 *
KRAUSE M: "Transcription and Translation", METHODS CELL BIOL, vol. 48, 1995, pages 483 - 512, XP001073411 *
WIRTZ E ET AL: "Gene expression mediated by bacteriophage T3 and T7 RNA polymerases in transgenic trypanosomes", NUCLEIC ACIDS RESEARCH, vol. 22, no. 19, 1994, pages 3887 - 3894, XP002196041 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7538095B2 (en) 1997-12-23 2009-05-26 The Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
US7888325B2 (en) 1999-01-28 2011-02-15 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
US8148345B2 (en) 1999-01-28 2012-04-03 Georgia Health Sciences University Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
US9051566B2 (en) 2001-01-31 2015-06-09 Alnylam Pharmaceuticals, Inc. Post-transcriptional gene silencing using expressed double stranded RNA

Also Published As

Publication number Publication date
GB0012231D0 (en) 2000-07-12
GB2362383A (en) 2001-11-21
WO2001088114A2 (en) 2001-11-22
AU7567101A (en) 2001-11-26
GB2362383B (en) 2003-12-31

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