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WO2001086303A3 - Rapid tests for herbicide resistance in grasses - Google Patents

Rapid tests for herbicide resistance in grasses Download PDF

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Publication number
WO2001086303A3
WO2001086303A3 PCT/GB2001/002068 GB0102068W WO0186303A3 WO 2001086303 A3 WO2001086303 A3 WO 2001086303A3 GB 0102068 W GB0102068 W GB 0102068W WO 0186303 A3 WO0186303 A3 WO 0186303A3
Authority
WO
WIPO (PCT)
Prior art keywords
antiserum
target polypeptide
grasses
herbicide resistance
weed
Prior art date
Application number
PCT/GB2001/002068
Other languages
French (fr)
Other versions
WO2001086303A2 (en
Inventor
John Reade
Andrew Howard Cobb
Original Assignee
Harper Adams University Colleg
John Reade
Andrew Howard Cobb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harper Adams University Colleg, John Reade, Andrew Howard Cobb filed Critical Harper Adams University Colleg
Priority to AU2001256481A priority Critical patent/AU2001256481A1/en
Publication of WO2001086303A2 publication Critical patent/WO2001086303A2/en
Publication of WO2001086303A3 publication Critical patent/WO2001086303A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/9116—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • G01N2333/91165—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5) general (2.5.1)
    • G01N2333/91171—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5) general (2.5.1) with definite EC number (2.5.1.-)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A method for use in detecting the presence of a target polypeptide in a weed, wherein the target polypeptide is connected with resistance of the weed to herbicides. The method comprises delivering to a sample of the weed an antiserum which is directed against the target polypeptide. The antiserum identifies and binds with the target polypeptide and the bound antiserum is then visualised by an appropriate method. The target polypeptide is preferably a glutathione S-transferase and the antiserum is preferably a monoclonal antiserum raised against polypeptide.
PCT/GB2001/002068 2000-05-11 2001-05-11 Rapid tests for herbicide resistance in grasses WO2001086303A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001256481A AU2001256481A1 (en) 2000-05-11 2001-05-11 Rapid tests for herbicide resistance in grasses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0011268.0 2000-05-11
GB0011268A GB0011268D0 (en) 2000-05-11 2000-05-11 Rapid tests for herbicide resistance in grasses

Publications (2)

Publication Number Publication Date
WO2001086303A2 WO2001086303A2 (en) 2001-11-15
WO2001086303A3 true WO2001086303A3 (en) 2002-04-04

Family

ID=9891323

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/002068 WO2001086303A2 (en) 2000-05-11 2001-05-11 Rapid tests for herbicide resistance in grasses

Country Status (3)

Country Link
AU (1) AU2001256481A1 (en)
GB (1) GB0011268D0 (en)
WO (1) WO2001086303A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2102654T3 (en) 2007-01-07 2015-01-30 Univ Aarhus Method and kit for detecting herbicide resistance in plants
GB0717982D0 (en) * 2007-09-14 2007-10-24 Univ Durham Method and means relating to multiple herbicide resistance in plants

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999014337A2 (en) * 1997-09-16 1999-03-25 Rhone-Poulenc Agriculture Limited New plant genes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999014337A2 (en) * 1997-09-16 1999-03-25 Rhone-Poulenc Agriculture Limited New plant genes

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CUMMINS IAN ET AL: "A role for glutathione transferases functioning as glutathione peroxidases in resistance to multiple herbicides in black-grass.", PLANT JOURNAL, vol. 18, no. 3, May 1999 (1999-05-01), pages 285 - 292, XP002181935, ISSN: 0960-7412 *
CUMMINS IAN ET AL: "Glutathione transferases in herbicide-resistant and herbicide-susceptible black-grass (Alopecurus myosuroides).", PESTICIDE SCIENCE, vol. 51, no. 3, November 1997 (1997-11-01), pages 244 - 250, XP001031131, ISSN: 0031-613X *
DATABASE SWISS PROT 1 May 1999 (1999-05-01), CUMMINS I ET AL.: "GSTs and herbicide resistance", XP002181937 *
DATABASE SWISS PROT 1 May 2000 (2000-05-01), CUMMINS I ET AL.: "GSTs and herbicide resistance", XP002181938 *
READE JOHN P H ET AL: "Glutathione S-transferases in black-grass (Alopecurus myosuroides Huds.): Properties and involvement in herbicide resistance.", PESTICIDE SCIENCE, vol. 55, no. 3, March 1999 (1999-03-01), pages 349 - 351, XP002181936, ISSN: 0031-613X *
READE JOHN P H ET AL: "Purification, characterization and comparison of glutathione S-transferases from black-grass (Alopecurus myosuroides Huds) biotypes.", PESTICIDE SCIENCE, vol. 55, no. 10, October 1999 (1999-10-01), pages 993 - 999, XP002181934, ISSN: 0031-613X *
READE, J. P. H. ET AL: "Rapid tests for herbicide resistance in black-grass based on elevated glutathione S-transferase activity and abundance", BRIGHTON CONF.--WEEDS (1999), (VOL. 1), 185-190, XP001039640 *

Also Published As

Publication number Publication date
GB0011268D0 (en) 2000-06-28
AU2001256481A1 (en) 2001-11-20
WO2001086303A2 (en) 2001-11-15

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