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WO2001077386A3 - Detection a haut rendement de mutations d'adn inconnues par electrophorese capillaire multiplex - Google Patents

Detection a haut rendement de mutations d'adn inconnues par electrophorese capillaire multiplex Download PDF

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Publication number
WO2001077386A3
WO2001077386A3 PCT/US2001/010893 US0110893W WO0177386A3 WO 2001077386 A3 WO2001077386 A3 WO 2001077386A3 US 0110893 W US0110893 W US 0110893W WO 0177386 A3 WO0177386 A3 WO 0177386A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
dna samples
mutations
electrophoresis
multiplexed
Prior art date
Application number
PCT/US2001/010893
Other languages
English (en)
Other versions
WO2001077386A2 (fr
Inventor
Edward S Yeung
Quifeng Gao
Original Assignee
Univ Iowa State Res Found Inc
Edward S Yeung
Quifeng Gao
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Iowa State Res Found Inc, Edward S Yeung, Quifeng Gao filed Critical Univ Iowa State Res Found Inc
Priority to AU2001249829A priority Critical patent/AU2001249829A1/en
Publication of WO2001077386A2 publication Critical patent/WO2001077386A2/fr
Publication of WO2001077386A3 publication Critical patent/WO2001077386A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne une méthode permettant de détecter par électrophorèse capillaire la présence de mutations dans des échantillons d'ADN. Cette méthode consiste: (a) à introduire séparément chaque échantillon d'ADN, qui contiennent tous une solution tampon comprenant une matrice et dont au moins un contient un ADN hétéroduplex, dans une pluralité de récipients dans un système d'électrophorèse ; (b) à chauffer les échantillons d'ADN indépendamment du champ électrique dans le système d'électrophorèse dans au moins une gamme spatiale ou temporelle prédéterminée de température choisie pour dénaturer l'échantillon d'ADN ; et (c) à détecter la présence de mutations dans les échantillons d'ADN en comparant un motif électrophorétique pour chaque échantillon d'ADN avec un motif électrophorétique pour un échantillon d'ADN témoin, ce qui permet de détecter par électrophorèse multiplex la présence de mutations dans des échantillons d'ADN. Ce procédé peut également consister au point (b) à introduire séparément au moins un échantillon d'ADN témoin, qui contient un ADN hétéroduplex, dans une solution tampon comprenant une matrice, dans un récipient de la pluralité de récipients du système d'électrophorèse.
PCT/US2001/010893 2000-04-05 2001-04-04 Detection a haut rendement de mutations d'adn inconnues par electrophorese capillaire multiplex WO2001077386A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001249829A AU2001249829A1 (en) 2000-04-05 2001-04-04 High-throughput detection of unknown dna mutations by using multiplexed capillary electrophoresis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19459700P 2000-04-05 2000-04-05
US60/194,597 2000-04-05

Publications (2)

Publication Number Publication Date
WO2001077386A2 WO2001077386A2 (fr) 2001-10-18
WO2001077386A3 true WO2001077386A3 (fr) 2003-01-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/010893 WO2001077386A2 (fr) 2000-04-05 2001-04-04 Detection a haut rendement de mutations d'adn inconnues par electrophorese capillaire multiplex

Country Status (2)

Country Link
AU (1) AU2001249829A1 (fr)
WO (1) WO2001077386A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1320745A4 (fr) 2000-09-01 2008-10-01 Applera Corp Systeme et procede d'electrophorese capillaire a gradient de temperature
US6872530B2 (en) 2002-04-24 2005-03-29 Spectrumedix, Llc Method for determining the presence of DNA variants using peptide nucleic acid probes
US7303879B2 (en) 2003-07-31 2007-12-04 Applera Corporation Determination of SNP allelic frequencies using temperature gradient electrophoresis
US20090294287A1 (en) * 2005-05-24 2009-12-03 Ebara Corporation Microchip electrophoresis method and device

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991002815A1 (fr) * 1989-08-19 1991-03-07 Diagen Institut Für Molekularbiologische Diagnostik Gmbh Procede et dispositif permettant de separer et de detecter les elements d'un melange de substances par electrophorese a gel a gradient de temperature
US5066377A (en) * 1986-07-04 1991-11-19 Diagen Institute fur molekularbiologische Diagnostik GmbH Method and device for producing a controllable and reproducible temperature gradient and use thereof
US5410412A (en) * 1990-05-14 1995-04-25 Labintellegence, Inc. Gel electrophoresis system
WO1996024687A1 (fr) * 1995-02-10 1996-08-15 University College London Detection de mutations ou de modifications dans l'adn
US5741411A (en) * 1995-05-19 1998-04-21 Iowa State University Research Foundation Multiplexed capillary electrophoresis system

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5066377A (en) * 1986-07-04 1991-11-19 Diagen Institute fur molekularbiologische Diagnostik GmbH Method and device for producing a controllable and reproducible temperature gradient and use thereof
WO1991002815A1 (fr) * 1989-08-19 1991-03-07 Diagen Institut Für Molekularbiologische Diagnostik Gmbh Procede et dispositif permettant de separer et de detecter les elements d'un melange de substances par electrophorese a gel a gradient de temperature
US5410412A (en) * 1990-05-14 1995-04-25 Labintellegence, Inc. Gel electrophoresis system
WO1996024687A1 (fr) * 1995-02-10 1996-08-15 University College London Detection de mutations ou de modifications dans l'adn
US5741411A (en) * 1995-05-19 1998-04-21 Iowa State University Research Foundation Multiplexed capillary electrophoresis system

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BARTA C ET AL: "Simultaneous analysis of various mutations on the 21-hydroxylase gene by multi-allele specific amplification and capillary gel electrophoresis", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER SCIENCE, NL, vol. 817, no. 1-2, 21 August 1998 (1998-08-21), pages 281 - 286, XP004139738, ISSN: 0021-9673 *
GONG X ET AL: "Genetic typing and HIV-1 diagnosis by using 96 capillary array electrophoresis and ultraviolet absorption detection", JOURNAL OF CHROMATOGRAPHY. BIOMEDICAL APPLICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 741, no. 1, April 2000 (2000-04-01), pages 15 - 21, XP004197021, ISSN: 0378-4347 *
NATARAJ ARUN J ET AL: "Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection", ELECTROPHORESIS, WEINHEIM, DE, vol. 20, no. 6, June 1999 (1999-06-01), pages 1177 - 1185, XP002192855, ISSN: 0173-0835 *

Also Published As

Publication number Publication date
WO2001077386A2 (fr) 2001-10-18
AU2001249829A1 (en) 2001-10-23

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