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WO2001077350A3 - Compositions and methods for inhibiting gene expression - Google Patents

Compositions and methods for inhibiting gene expression Download PDF

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Publication number
WO2001077350A3
WO2001077350A3 PCT/US2001/011436 US0111436W WO0177350A3 WO 2001077350 A3 WO2001077350 A3 WO 2001077350A3 US 0111436 W US0111436 W US 0111436W WO 0177350 A3 WO0177350 A3 WO 0177350A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
present
eukaryotic cell
endogenous gene
compositions
Prior art date
Application number
PCT/US2001/011436
Other languages
French (fr)
Other versions
WO2001077350A2 (en
Inventor
Kenneth E Palmer
Gregory P Pogue
Original Assignee
Large Scale Biology Corp
Kenneth E Palmer
Gregory P Pogue
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Large Scale Biology Corp, Kenneth E Palmer, Gregory P Pogue filed Critical Large Scale Biology Corp
Priority to AU2001253255A priority Critical patent/AU2001253255A1/en
Publication of WO2001077350A2 publication Critical patent/WO2001077350A2/en
Publication of WO2001077350A3 publication Critical patent/WO2001077350A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides an eukaryotic recombinant vector suited for bi-directional transcription of a transgene to yield both sense and antisense RNA transcripts of the transgene in an eukaryotic cell. The invention vectors are particularly suited for mediating gene silencing in a variety of biological systems. The present invention also provides host cells and transgenic plants comprising the invention vectors. Further provided by the invention are methods of inhibiting expression of an endogenous gene present in an eukaryotic cell. Also included is a method of identifying a biological function(s) of an endogenous gene of interest in an eukaryotic cell by selectively inhibiting the expression of the endogenous gene.
PCT/US2001/011436 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression WO2001077350A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001253255A AU2001253255A1 (en) 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54557400A 2000-04-07 2000-04-07
US09/545,574 2000-04-07

Publications (2)

Publication Number Publication Date
WO2001077350A2 WO2001077350A2 (en) 2001-10-18
WO2001077350A3 true WO2001077350A3 (en) 2002-08-08

Family

ID=24176765

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/011436 WO2001077350A2 (en) 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression

Country Status (2)

Country Link
AU (1) AU2001253255A1 (en)
WO (1) WO2001077350A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6911541B2 (en) 2000-08-30 2005-06-28 North Carolina State University Promoter fragment that is recognized by the product of the tobacco Nic gene
US7189570B2 (en) 2000-11-07 2007-03-13 North Carolina State University Putrescine-n-methyltransferase promoter
US7304220B2 (en) 1997-06-12 2007-12-04 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression

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AU2001297906B2 (en) * 2000-10-31 2007-07-19 Commonwealth Scientific And Industrial Research Organisation Method and means for producing barley yellow dwarf virus resistant cereal plants
SG132542A1 (en) 2001-06-08 2007-06-28 Vector Tobacco Ltd Modifying nicotine and nitrosamine levels in tobacco
CA2465860A1 (en) 2001-11-02 2004-04-22 Insert Therapeutics, Inc. Methods and compositions for therapeutic use of rna interference
AU2003283951A1 (en) 2002-05-23 2004-03-03 Ceptyr, Inc. Modulation of biological signal transduction by rna interference
US7148342B2 (en) 2002-07-24 2006-12-12 The Trustees Of The University Of Pennyslvania Compositions and methods for sirna inhibition of angiogenesis
AU2002951347A0 (en) * 2002-09-09 2002-09-26 Benitec Australia Ltd Genetic silencing
DE602004014485D1 (en) 2003-04-07 2008-07-31 Prospect Therapeutics Inc COMPOSITION AND APPLICATIONS OF GALECTIN ANTAGONISTS
WO2005097207A2 (en) 2004-03-26 2005-10-20 Curis, Inc. Rna interference modulators of hedgehog signaling and uses thereof
KR100912994B1 (en) 2004-04-05 2009-08-21 더 리전트 오브 더 유니버시티 오브 캘리포니아 Modulation of nkg2d
AU2005275062A1 (en) 2004-07-14 2006-02-23 University Of Utah Research Foundation Netrin-related compositions and uses
US20060063208A1 (en) 2004-08-02 2006-03-23 Woolf Clifford J DRG11-responsive (DRAGON) gene and uses thereof
US7517870B2 (en) 2004-12-03 2009-04-14 Fondazione Telethon Use of compounds that interfere with the hedgehog signaling pathway for the manufacture of a medicament for preventing, inhibiting, and/or reversing ocular diseases related with ocular neovascularization
JP2008537551A (en) 2005-03-31 2008-09-18 カランド ファーマシューティカルズ， インコーポレイテッド Inhibitors of ribonucleotide reductase subunit 2 and uses thereof
US8715665B2 (en) 2007-04-13 2014-05-06 The General Hospital Corporation Methods for treating cancer resistant to ErbB therapeutics
AU2008256644B2 (en) 2007-05-24 2014-07-03 The United States Government As Represented By The Department Of Veterans Affairs Intranuclear protein transduction through a nucleoside salvage pathway
KR20100127880A (en) 2008-04-04 2010-12-06 카란도 파마슈티칼즈, 인코포레이티드 Compositions and Uses of EPAS1 Inhibitors
WO2010065834A1 (en) 2008-12-04 2010-06-10 Opko Ophthalmics, Llc Compositions and methods for selective inhibition of pro-angiogenic vegf isoforms
IT1398977B1 (en) 2009-06-25 2013-03-28 Icgeb ANTI-PANT AGENT SPECIFIC FOR CEREBRAL CANCERS WITH RECQ1 SUPPRESSION MECHANISM
GB201103167D0 (en) 2011-02-24 2011-04-06 Isis Innovation Gene silencing
US9636370B2 (en) 2012-09-28 2017-05-02 The University Of North Carolina At Chapel Hill AAV vectors targeted to oligodendrocytes
JP6930913B2 (en) 2014-10-14 2021-09-01 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ Usage of CDK9 and BRD4 inhibitors to inhibit inflammation
CN107532173A (en) 2014-11-21 2018-01-02 北卡罗来纳-查佩尔山大学 Target the AAV carriers of central nervous system
CA2969857A1 (en) 2014-12-05 2016-06-09 Case Western Reserve University Compositions and methods of modulating s-nitrosylation
WO2016127057A1 (en) 2015-02-06 2016-08-11 The University Of North Carolina At Chapel Hill Optimized human clotting factor viii gene expression cassettes and their use
US10500273B2 (en) 2015-03-02 2019-12-10 180 Therapeutics Lp Method of treating a localized fibrotic disorder using an IL-33 antagonist
CA3031818A1 (en) 2016-07-27 2018-02-01 Case Western Reserve University Compounds and methods of promoting myelination
CN110249051A (en) 2016-12-08 2019-09-17 凯斯西储大学 Enhance the method and composition that functional myelin generates
CN119242659A (en) 2018-06-12 2025-01-03 北卡罗来纳大学教堂山分校 Synthetic hepatotropic adeno-associated virus capsid and its use
CA3128230A1 (en) 2019-01-31 2020-08-06 Oregon Health & Science University Methods for using transcription-dependent directed evolution of aav capsids

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ROGERS S A ET AL: "ANALYSIS OF CHLOROPLAST PROMOTERS USING BIDIRECTIONAL TRANSCRIPTION VECTORS", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 15, no. 3, 1990, pages 421 - 435, XP002159238, ISSN: 0167-4412 *
SPICER D B ET AL: "AN ANTISENSE PROMOTER OF THE MURINE C-MYC GENE IS LOCALIZED WITHIN INTRON 2", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 12, no. 3, March 1992 (1992-03-01), pages 1324 - 1329, XP002910389, ISSN: 0270-7306 *
SUREAU ALAIN ET AL: "Characterization of multiple alternative RNAs resulting from antisense transcription of the PR264/SC35 splicing factor gene.", NUCLEIC ACIDS RESEARCH, vol. 25, no. 22, 15 November 1997 (1997-11-15), pages 4513 - 4522, XP002197926, ISSN: 0305-1048 *
VELHAGEN IRIS ET AL: "An antisense promoter within the hepatitis B virus X gene.", INTERVIROLOGY, vol. 38, no. 3-4, 1995, pages 127 - 133, XP008002810, ISSN: 0300-5526 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7304220B2 (en) 1997-06-12 2007-12-04 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US6911541B2 (en) 2000-08-30 2005-06-28 North Carolina State University Promoter fragment that is recognized by the product of the tobacco Nic gene
US7192771B2 (en) 2000-08-30 2007-03-20 North Carolina State University Plant promoter sequence
US7189570B2 (en) 2000-11-07 2007-03-13 North Carolina State University Putrescine-n-methyltransferase promoter

Also Published As

Publication number Publication date
WO2001077350A2 (en) 2001-10-18
AU2001253255A1 (en) 2001-10-23

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