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WO2001073038A3 - Method for the biotechnological production of l-alaninol - Google Patents

Method for the biotechnological production of l-alaninol Download PDF

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Publication number
WO2001073038A3
WO2001073038A3 PCT/EP2001/003651 EP0103651W WO0173038A3 WO 2001073038 A3 WO2001073038 A3 WO 2001073038A3 EP 0103651 W EP0103651 W EP 0103651W WO 0173038 A3 WO0173038 A3 WO 0173038A3
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WO
WIPO (PCT)
Prior art keywords
alaninol
biotechnological production
organisms
production
novel micro
Prior art date
Application number
PCT/EP2001/003651
Other languages
German (de)
French (fr)
Other versions
WO2001073038A2 (en
Inventor
Thomas Leisinger
Der Ploeg Jan Van
Andreas M Kiener
Azevedo Waesch Susana Ivone De
Tere Maire
Original Assignee
Lonza Ag
Thomas Leisinger
Der Ploeg Jan Van
Andreas M Kiener
Azevedo Waesch Susana Ivone De
Tere Maire
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Ag, Thomas Leisinger, Der Ploeg Jan Van, Andreas M Kiener, Azevedo Waesch Susana Ivone De, Tere Maire filed Critical Lonza Ag
Priority to AU46521/01A priority Critical patent/AU4652101A/en
Publication of WO2001073038A2 publication Critical patent/WO2001073038A2/en
Publication of WO2001073038A3 publication Critical patent/WO2001073038A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Disclosed are novel micro-organisms which are capable of transforming isopropylamine into L-alaninol and wherein the genes ipuH and ipul coding for enzymes involved in the metabolization of L-alaninol are deactivated. The invention also relates to a method for the production of L-alaninol or theanine using said novel micro-organisms.
PCT/EP2001/003651 2000-03-31 2001-03-30 Method for the biotechnological production of l-alaninol WO2001073038A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU46521/01A AU4652101A (en) 2000-03-31 2001-04-03 Method for the biotechnological production of l-alaninol

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00106888 2000-03-31
EP00106888.1 2000-03-31

Publications (2)

Publication Number Publication Date
WO2001073038A2 WO2001073038A2 (en) 2001-10-04
WO2001073038A3 true WO2001073038A3 (en) 2002-10-24

Family

ID=8168302

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/003651 WO2001073038A2 (en) 2000-03-31 2001-03-30 Method for the biotechnological production of l-alaninol

Country Status (2)

Country Link
AU (1) AU4652101A (en)
WO (1) WO2001073038A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2706463A1 (en) 2007-12-20 2009-07-02 Basf Se Process for the preparation of (s)-2-amino-1-propanol (l-alaninol) from (s)-1-methoxy-2-propylamine

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0652291A1 (en) * 1992-07-07 1995-05-10 Fuso Pharmaceutical Industries Ltd. Probe for diagnosing infectious disease
WO1999007199A2 (en) * 1997-08-08 1999-02-18 Lonza Ag Method for producing l-alaninol and gamma-glutamyl isopropylamide and a microbial strain of the pseudomonas

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0652291A1 (en) * 1992-07-07 1995-05-10 Fuso Pharmaceutical Industries Ltd. Probe for diagnosing infectious disease
WO1999007199A2 (en) * 1997-08-08 1999-02-18 Lonza Ag Method for producing l-alaninol and gamma-glutamyl isopropylamide and a microbial strain of the pseudomonas

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
DATABASE EM_HTG EMBL; 16 March 1998 (1998-03-16), ROE B A: "Pseudomonas sp. chromosome genomic clone cos3b, WORKING DRAFT SEQUENCE, 2 unordered pieces", XP002189827 *
DATABASE EM_PRO EMBL; 14 March 2000 (2000-03-14), KURUSU Y: "Pseudomonas putida icd, mutS, fdxA genes for isocitrate dehydrogenase, mismatch repair system component MutS, ferredoxin, partial and complete cds", XP002189826 *
DATABASE EM_PRO EMBL; 4 January 1999 (1999-01-04), KIM S ET AL.: "Pseudomonas sp. DJ77 4-hydroxy-2-ketovalerate aldolase (phnJ) gene, partial cds; and 4-oxalocrotonate decarboxylase (phnK), 4-oxalocrotonate isomerase (phnL), plant-type 2Fe-2S ferredoxin (phnM), and benzaldehyde dehydrogenase (phnN) genes, complete cds", XP002189828 *
DATABASE SWALL EMBL; 1 January 1998 (1998-01-01), EATON R W: "p-Cumic aldehyde dehydrogenase", XP002189831 *
DATABASE SWALL EMBL; 1 November 1996 (1996-11-01), MADHUSUDHAN K T ET AL.: "Hypothetical protein in bkdA1 5' region (fragment)", XP002189829 *
DATABASE SWALL EMBL; 21 July 1986 (1986-07-21), UNGER B P ET AL.: "Cytochrome P450-cam (EC 1.14.15.1) (camphor 5-monooxygenase) (P450cam)", XP002189830 *
IURESCIA S ET AL.: "Identification and sequencing of beta-myrcene catabolism genes from Pseudomonas sp. strain M1.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 7, July 1999 (1999-07-01), pages 2871 - 2876, XP002180238, ISSN: 0099-2240 *
JONES A ET AL.: "Microbial metabolism of amino alcohols - 1-aminopropan-2-ol and ethanolamine metabolism via propionaldehyde and acetaldehyde in a Pseudomonas sp.", BIOCHEMICAL JOURNAL, vol. 134, no. 1, 1973, pages 167 - 182, XP001030697, ISSN: 0264-6021 *
PEROZICH J ET AL.: "Relationships within the aldehyde dehydrogenase extended family.", PROTEIN SCIENCE, vol. 8, no. 1, January 1999 (1999-01-01), pages 137 - 146, XP001030674, ISSN: 0961-8368 *
TACHIKI T ET AL.: "gamma-Glutamyl transfer reactions by glutaminase from Pseudomonas nitroreducens IFO 12694 and their application for the syntheses of theanine and gamma-glutamylmethylamide.", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 62, no. 7, July 1998 (1998-07-01), pages 1279 - 1283, XP002180239, ISSN: 0916-8451 *
TACHIKI T ET AL.: "Production of gamma-glutamylethylamide and gamma-glutamylmethylamide by coupling of baker's yeast preparations and bacterial glutamine synthetase", JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, vol. 32, no. 6, December 1986 (1986-12-01), pages 545 - 548, XP001030636, ISSN: 0022-1260 *
TACHIKI T ET AL: "Purification and some properties of glutaminase from Pseudomonas nitroreducens IFO 12694.", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 60, no. 7, 1996, pages 1160 - 1164, XP002189825, ISSN: 0916-8451 *

Also Published As

Publication number Publication date
AU4652101A (en) 2001-10-08
WO2001073038A2 (en) 2001-10-04

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