WO2001072813A1 - Nouveau polypeptide, facteur humain 6-14 de couplage de l'atpase mitochondriale, et polynucleotide codant pour ce polypeptide - Google Patents
Nouveau polypeptide, facteur humain 6-14 de couplage de l'atpase mitochondriale, et polynucleotide codant pour ce polypeptide Download PDFInfo
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- WO2001072813A1 WO2001072813A1 PCT/CN2001/000480 CN0100480W WO0172813A1 WO 2001072813 A1 WO2001072813 A1 WO 2001072813A1 CN 0100480 W CN0100480 W CN 0100480W WO 0172813 A1 WO0172813 A1 WO 0172813A1
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- Prior art keywords
- polypeptide
- polynucleotide
- coupling factor
- human mitochondrial
- mitochondrial atpase
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
Definitions
- the present invention provides a new polypeptide, human mitochondrial ATPase coupling factor 6-14, which is basically composed of the amino acid sequence shown in SEQ ID NO: 2.
- the polypeptide of the present invention may be a recombinant polypeptide, a natural polypeptide, or a synthetic polypeptide, and preferably a recombinant polypeptide.
- the polypeptides of the present invention can be naturally purified products or chemically synthesized products, or can be produced from prokaryotic or eukaryotic hosts (eg, bacteria, yeast, higher plants, insects, and mammalian cells) using recombinant techniques.
- the invention provides an isolated nucleic acid (polynucleotide), which basically consists of a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 2.
- the polynucleotide sequence of the present invention includes the nucleotide sequence of SEQ ID NO: 1.
- the polynucleotide of the present invention is found from a cDNA library of human fetal brain tissue. It contains a polynucleotide sequence that is 897 bases in length and its open reading frame 157-552 encodes 1 31 amino acids.
- Methods known to those skilled in the art can be used to construct expression vectors containing DNA sequences encoding human mitochondrial ATPase coupling factor 6-14 and appropriate transcriptional / translational regulatory elements. These methods include in vitro recombinant DM technology, DNA synthesis technology, in vivo recombination technology, etc. (Sambroook, et'al. Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory. New York, 1989).
- the DNA sequence can be operably linked to an appropriate promoter in the expression vector to guide mRNA synthesis. Representative examples of these promoters are: the lac or trp promoter of E.
- Dye terminate cycle reaction ion sequencing kit Perkin-Elraer
- ABI 377 automatic sequencer Perkin-Elmer
- the determined cDNA sequence was compared with the existing public DNA sequence database (Genebank), and it was found that the cDNA sequence of one of the clones 0065f05 was new DNA.
- a series of primers were synthesized to determine the inserted cDNA fragments of the clone in both directions.
- Primerl 5, — GGATGTGTAGCTTTGGAAATCTGT —3, (SEQ ID NO: 3)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention concerne un nouveau polypeptide, un facteur humain 6-14 de couplage de l'ATPase mitochondriale, et un polynucléotide codant pour ce polypeptide ainsi qu'un procédé d'obtention de ce polypeptide par des techniques recombinantes d'ADN. L'invention concerne en outre les applications de ce polypeptide dans le traitement de maladies, notamment des tumeurs malignes, de l'hémopathie, de l'infection par VIH, de maladies immunitaires et de diverses inflammations. L'invention concerne aussi l'antagoniste agissant contre le polypeptide et son action thérapeutique ainsi que les applications de ce polynucléotide codant pour le facteur humain 6-14 de couplage de l'ATPase mitochondriale.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU63718/01A AU6371801A (en) | 2000-03-28 | 2001-03-26 | A novel polypeptide-human mitochondrial atpase coupling factor 6-14 and a polynucleotide sequence encoding the same |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00115195 CN1315396A (zh) | 2000-03-28 | 2000-03-28 | 一种新的多肽——人线粒体atp酶耦合因子6－14和编码这种多肽的多核苷酸 |
| CN00115195.9 | 2000-03-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2001072813A1 true WO2001072813A1 (fr) | 2001-10-04 |
Family
ID=4584663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2001/000480 WO2001072813A1 (fr) | 2000-03-28 | 2001-03-26 | Nouveau polypeptide, facteur humain 6-14 de couplage de l'atpase mitochondriale, et polynucleotide codant pour ce polypeptide |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN1315396A (fr) |
| AU (1) | AU6371801A (fr) |
| WO (1) | WO2001072813A1 (fr) |
-
2000
- 2000-03-28 CN CN 00115195 patent/CN1315396A/zh active Pending
-
2001
- 2001-03-26 WO PCT/CN2001/000480 patent/WO2001072813A1/fr active Application Filing
- 2001-03-26 AU AU63718/01A patent/AU6371801A/en not_active Abandoned
Non-Patent Citations (4)
| Title |
|---|
| BIOCHIM. BIOPHYS. ACTA, vol. 1089, no. 3, 1991, pages 393 - 395 * |
| GENE, vol. 116, no. 2, 1992, pages 291 - 292 * |
| GENE, vol. 97, no. 2, 1991, pages 307 - 310 * |
| J. BIOL. CHEM., vol. 262, no. 7, 1987, pages 3007 - 3010 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU6371801A (en) | 2001-10-08 |
| CN1315396A (zh) | 2001-10-03 |
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