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WO2001072392A3 - New methods for separation and isolation of subpopulations of cells in biological samples - Google Patents

New methods for separation and isolation of subpopulations of cells in biological samples Download PDF

Info

Publication number
WO2001072392A3
WO2001072392A3 PCT/IB2001/001461 IB0101461W WO0172392A3 WO 2001072392 A3 WO2001072392 A3 WO 2001072392A3 IB 0101461 W IB0101461 W IB 0101461W WO 0172392 A3 WO0172392 A3 WO 0172392A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
isolation
separation
biological samples
subpopulations
Prior art date
Application number
PCT/IB2001/001461
Other languages
French (fr)
Other versions
WO2001072392A2 (en
Inventor
Eva Irene Stenbaek Jauho
Mogens Havsteen Jakobsen
Original Assignee
Exiqon As
Eva Irene Stenbaek Jauho
Mogens Havsteen Jakobsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exiqon As, Eva Irene Stenbaek Jauho, Mogens Havsteen Jakobsen filed Critical Exiqon As
Priority to AU2001277635A priority Critical patent/AU2001277635A1/en
Publication of WO2001072392A2 publication Critical patent/WO2001072392A2/en
Publication of WO2001072392A3 publication Critical patent/WO2001072392A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The present invention relates to novel methods for separation, isolation and recovery of sub-populations of cells (D) in biological samples. Ligands (B, C) suitable for the detection of the desired cell type are covalently attached to the surface of a non-porous body by the use of a method comprising a step of photochemical coupling of a thermochemically active molecule (A), e.g. anthraquinone, to the surface. In a presently preferred embodiment, the invention provides to methods for separation, isolation and recovery of foetal cells from maternal blood by use of specific cell markers for specific subpopulations of foetal cells. The invention further relates to a kit for use in the methods described.
PCT/IB2001/001461 2000-03-14 2001-03-13 New methods for separation and isolation of subpopulations of cells in biological samples WO2001072392A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001277635A AU2001277635A1 (en) 2000-03-14 2001-03-13 New methods for separation and isolation of subpopulations of cells in biological samples

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200000416 2000-03-14
DKPA200000416 2000-03-14
US18958900P 2000-03-15 2000-03-15
US60/189,589 2000-03-15

Publications (2)

Publication Number Publication Date
WO2001072392A2 WO2001072392A2 (en) 2001-10-04
WO2001072392A3 true WO2001072392A3 (en) 2002-05-23

Family

ID=26068793

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2001/001461 WO2001072392A2 (en) 2000-03-14 2001-03-13 New methods for separation and isolation of subpopulations of cells in biological samples

Country Status (2)

Country Link
AU (1) AU2001277635A1 (en)
WO (1) WO2001072392A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5447842A (en) * 1990-03-27 1995-09-05 Genetype A.G. Fetal cell recovery method
WO1996031557A1 (en) * 1995-04-07 1996-10-10 Mogens Havsteen Jacobsen Method of photochemical immobilization of ligands using quinones
WO1999015628A1 (en) * 1997-09-25 1999-04-01 Glycotech Corporation Methods and compositions for binding hematopoietic stem cells
WO2000036419A1 (en) * 1998-12-15 2000-06-22 Exiqon A/S Coupling of lipopolysaccharide-derived carbohydrates onto solid surfaces

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5447842A (en) * 1990-03-27 1995-09-05 Genetype A.G. Fetal cell recovery method
WO1996031557A1 (en) * 1995-04-07 1996-10-10 Mogens Havsteen Jacobsen Method of photochemical immobilization of ligands using quinones
WO1999015628A1 (en) * 1997-09-25 1999-04-01 Glycotech Corporation Methods and compositions for binding hematopoietic stem cells
WO2000036419A1 (en) * 1998-12-15 2000-06-22 Exiqon A/S Coupling of lipopolysaccharide-derived carbohydrates onto solid surfaces

Also Published As

Publication number Publication date
AU2001277635A1 (en) 2001-10-08
WO2001072392A2 (en) 2001-10-04

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