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WO2001058591A2 - Plaque d'analyse multipuits fermee servant a realiser des mesures optiques analytiques - Google Patents

Plaque d'analyse multipuits fermee servant a realiser des mesures optiques analytiques Download PDF

Info

Publication number
WO2001058591A2
WO2001058591A2 PCT/EP2000/001003 EP0001003W WO0158591A2 WO 2001058591 A2 WO2001058591 A2 WO 2001058591A2 EP 0001003 W EP0001003 W EP 0001003W WO 0158591 A2 WO0158591 A2 WO 0158591A2
Authority
WO
WIPO (PCT)
Prior art keywords
sealing material
analysis plate
plate according
base body
openings
Prior art date
Application number
PCT/EP2000/001003
Other languages
German (de)
English (en)
Other versions
WO2001058591A3 (fr
Inventor
Anton Horn
Stefan Kreusch
Thomas Moore
Original Assignee
Cybio Instruments Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cybio Instruments Gmbh filed Critical Cybio Instruments Gmbh
Priority to AU2000229083A priority Critical patent/AU2000229083A1/en
Priority to PCT/EP2000/001003 priority patent/WO2001058591A2/fr
Publication of WO2001058591A2 publication Critical patent/WO2001058591A2/fr
Publication of WO2001058591A3 publication Critical patent/WO2001058591A3/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids

Definitions

  • the invention relates to a closed multiwell analysis plate for analytical optical measurements.
  • multiwell technology has become widely used in recent years (e.g. Matthews PD, Wurtzel ET: high-throughput microplate format for producing and screening riboprobes from bacterial cells, Biotechniques. 1995 Jun, 18 (6), 1000-1002, 1004 or Wu P., Daniel-Issakani S., LaMarco K., Strulovici B .: an automated high throughput filtration assay: application to polymerase inhibitor identification ,; anal- Biochem.
  • Gerasimova NS Steklova IV, Tuuminen T .: Fluorometric method for phenylalanine microplate assay adapted for phenylketonuria screening, Clin-Chem. 1989, Oct, 35 (10), 21 12-21 15 or Collins L, et al. Microplate alamar blue assay versus BACTEC 460 System for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrob Agents Chemother. 1997 May; 41 (5): 1004-9. Herrero ME, et al. Fluorometric microassay to quantify microsomal epoxide hydrolase in 96-well plates. Anal Biochem.
  • the radiation or the evaluated optical signals are completely or partially suppressed by the sealing material; at least there is inhomogeneous signal interference via the large number of individual collecting vessel openings in the multiwell analysis plate.
  • the sealing means are usually removed before the optical analysis and, if necessary, subsequently reapplied for transport or further treatment, which, however, is cumbersome and time-consuming, especially when removing films.
  • evaporation and contamination effects during the measuring process cannot be excluded.
  • the invention is based on the object of creating a multiwell analysis plate which can be handled easily and with little effort and which can also be used for fractions in the Microliter scale and analytical optical measurements, especially at elevated temperatures and over a longer period of time, largely falsified analysis results.
  • the collecting vessel openings of the multiwell analysis plate are closed with an optically permeable sealing material, for example a transparent sealing film made of plastic or a glass plate, the adhesives for fastening the sealing material covering the collecting vessel openings being exclusively at the contact points of the sealing material with the multiwell analysis plate, but not in the area of the collecting vessel openings.
  • an optically permeable sealing material for example a transparent sealing film made of plastic or a glass plate
  • the adhesives for fastening the sealing material covering the collecting vessel openings being exclusively at the contact points of the sealing material with the multiwell analysis plate, but not in the area of the collecting vessel openings.
  • Fig. 1 Multiwell analysis plate made of base body and sealing film in a perspective view
  • Fig. 2 Basic body and sealing film in several views 1 shows a perspective view of a multiwell analysis plate with a base body 1, which has openings 2 in collecting vessels for fractionating, analyzing, storing and transporting substances, etc. So that the openings 2 are sealed against evaporation and contamination protection, a flexible, translucent sealing film 3 is provided, which is placed on the base body 1 and fixed by annular adhesive surfaces 4. The adhesive surfaces 4 are arranged around the openings 2 or the boundary edges of the base body 1. There is no adhesive layer itself on the translucent sealing film 3.
  • the openings 2 of the receptacles are reliably closed, and when the sealing film 3 is applied, it is located exclusively on the contact surface between the sealing film 3 and the base body 1, ie on the edges of the openings 2 adhesive, but not in the area of the optical one Analysis analysis provided openings 2 of the collecting vessels.
  • FIG. 2 shows the base body 1 and the sealing film 3 separately in different views and together in a side view, the base body 1 in cross section (FIG. 2a) and in a top view (FIG. 2b) and the sealing film 3 likewise in cross section (FIG. 2c ) and are shown in plan view (Fig. 2d).
  • 2e shows the base body 1 closed with the sealing film 3 in cross section.
  • the sealing film 3 consists, as in FIG. 1, of translucent material, with the difference that it is not the base body 1 but the sealing film 3 which has annular adhesive surfaces 5 or also has adhesive surfaces on the edges which correspond to adhesive contact surfaces 6 of the basic body 1 , These adhesive contact surfaces 6, like the adhesive surfaces 4 in FIG.
  • the sealing film 3 In order to be able to apply the sealing film 3 with respect to the adhesive surfaces 5 and the position-assigned adhesive contact surfaces 6 on the base body 1 in an exact position, there are solitary structural elements 7 in the edge region of the base body 1 which engage in corresponding holes 8 in the sealing film 3.
  • the adhesive on the adhesive surfaces 4 and 5 is preferably heat-soluble or hardenable by UV radiation.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

L'invention concerne une plaque d'analyse multipuits fermée, servant à réaliser des mesures optiques analytiques, notamment dans le domaine de la biochimie, la protection de l'environnement et le diagnostic médical. L'invention vise à créer une plaque d'analyse multipuits pouvant être manipulée facilement et de façon économique, permettant d'obtenir des résultats d'analyse dans une large mesure non falsifiés, même pour des fractions de l'ordre du microlitre, lors de mesures optiques analytiques, notamment à des températures élevées et sur une longue période. A cet effet, les ouvertures du récipient collecteur de la plaque d'analyse multipuits sont fermées par un matériau d'étanchéité optiquement transparent, par exemple par un film transparent constitué de plastique ou d'une plaque de verre. Les adhésifs servant à fixer le matériau d'étanchéité qui recouvre les ouvertures du récipient collecteur se trouvent exclusivement au niveau des points de contact entre le matériau d'étanchéité et la plaque d'analyse multipuits, mais non dans la zone des ouvertures du récipient collecteur. Ni le matériau d'étanchéité ni ses moyens de fixation sur la plaque d'analyse multipuits, notamment des adhésifs pouvant être retirés sous l'effet de la chaleur, n'entravent l'entrée du rayonnement lumineux dans les échantillons ni la sortie dudit rayonnement de ces derniers, lors de la mesure optique analytique.
PCT/EP2000/001003 2000-02-08 2000-02-08 Plaque d'analyse multipuits fermee servant a realiser des mesures optiques analytiques WO2001058591A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2000229083A AU2000229083A1 (en) 2000-02-08 2000-02-08 Closed multi-well analytical plate with annular adhesive patches for analytical optical measurements
PCT/EP2000/001003 WO2001058591A2 (fr) 2000-02-08 2000-02-08 Plaque d'analyse multipuits fermee servant a realiser des mesures optiques analytiques

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2000/001003 WO2001058591A2 (fr) 2000-02-08 2000-02-08 Plaque d'analyse multipuits fermee servant a realiser des mesures optiques analytiques

Publications (2)

Publication Number Publication Date
WO2001058591A2 true WO2001058591A2 (fr) 2001-08-16
WO2001058591A3 WO2001058591A3 (fr) 2002-10-31

Family

ID=8163825

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/001003 WO2001058591A2 (fr) 2000-02-08 2000-02-08 Plaque d'analyse multipuits fermee servant a realiser des mesures optiques analytiques

Country Status (2)

Country Link
AU (1) AU2000229083A1 (fr)
WO (1) WO2001058591A2 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004038152A1 (de) * 2004-08-06 2006-02-23 Eppendorf Ag Abdeckfolie für eine Mikrotiterplatte
EP1937410A1 (fr) * 2005-10-18 2008-07-02 GE Healthcare Bio-Sciences AB Plaque multipuits
EP2040983A2 (fr) * 2006-06-26 2009-04-01 Applera Corporation Scellement transparent compressible pour microplaques ouvertes
JP2011047754A (ja) * 2009-08-26 2011-03-10 Shimadzu Corp 反応容器
WO2012010412A1 (fr) * 2010-07-19 2012-01-26 Boehringer Ingelheim Microparts Gmbh Dispositif microfluidique et son procédé de fabrication
EP2446967A1 (fr) * 2010-11-02 2012-05-02 F. Hoffmann-La Roche AG Système de traitement et/ou d'analyse d'échantillons liquides, agencement de fermeture et procédé pour thermosceller une plaque

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4444310A (en) * 1982-07-26 1984-04-24 Becton, Dickinson And Company Segmented multi-product package assembly
WO1992020448A1 (fr) * 1991-05-15 1992-11-26 Packard Instrument Company, Inc. Microplaquette pour le stockage d'echantillons radioactifs
GB9225307D0 (en) * 1992-12-03 1993-01-27 Norton David R Sealing films for multiwell plates,strips & microtubes
US5763263A (en) * 1995-11-27 1998-06-09 Dehlinger; Peter J. Method and apparatus for producing position addressable combinatorial libraries
DE19852946A1 (de) * 1998-11-12 2000-05-18 Univ Schiller Jena Verschlossene Multiwellanalysenplatte für analytische optische Messungen

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004038152A1 (de) * 2004-08-06 2006-02-23 Eppendorf Ag Abdeckfolie für eine Mikrotiterplatte
DE102004038152B4 (de) * 2004-08-06 2006-06-08 Eppendorf Ag Abdeckfolie für eine Mikrotiterplatte
EP1937410A1 (fr) * 2005-10-18 2008-07-02 GE Healthcare Bio-Sciences AB Plaque multipuits
EP1937410A4 (fr) * 2005-10-18 2014-05-21 Ge Healthcare Bio Sciences Ab Plaque multipuits
EP2040983A2 (fr) * 2006-06-26 2009-04-01 Applera Corporation Scellement transparent compressible pour microplaques ouvertes
EP2040983A4 (fr) * 2006-06-26 2011-08-03 Life Technologies Corp Scellement transparent compressible pour microplaques ouvertes
JP2011047754A (ja) * 2009-08-26 2011-03-10 Shimadzu Corp 反応容器
WO2012010412A1 (fr) * 2010-07-19 2012-01-26 Boehringer Ingelheim Microparts Gmbh Dispositif microfluidique et son procédé de fabrication
EP2446967A1 (fr) * 2010-11-02 2012-05-02 F. Hoffmann-La Roche AG Système de traitement et/ou d'analyse d'échantillons liquides, agencement de fermeture et procédé pour thermosceller une plaque

Also Published As

Publication number Publication date
WO2001058591A3 (fr) 2002-10-31
AU2000229083A1 (en) 2001-08-20
AU2000229083A8 (en) 2001-11-01

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