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WO2001053479A3 - Sequences activatrices ii - Google Patents

Sequences activatrices ii Download PDF

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Publication number
WO2001053479A3
WO2001053479A3 PCT/GB2001/000187 GB0100187W WO0153479A3 WO 2001053479 A3 WO2001053479 A3 WO 2001053479A3 GB 0100187 W GB0100187 W GB 0100187W WO 0153479 A3 WO0153479 A3 WO 0153479A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecule
ligand
candidate
binding
molecular switches
Prior art date
Application number
PCT/GB2001/000187
Other languages
English (en)
Other versions
WO2001053479A2 (fr
Inventor
Yen Choo
Christopher Graeme Ullman
Michael Moore
Original Assignee
Sangamo Biosciences Inc
Yen Choo
Christopher Graeme Ullman
Michael Moore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0001578A external-priority patent/GB0001578D0/en
Priority claimed from GB0001582A external-priority patent/GB0001582D0/en
Priority claimed from PCT/GB2000/002080 external-priority patent/WO2001000815A1/fr
Priority claimed from PCT/GB2000/002071 external-priority patent/WO2000073434A1/fr
Priority claimed from GBGB0029901.6A external-priority patent/GB0029901D0/en
Application filed by Sangamo Biosciences Inc, Yen Choo, Christopher Graeme Ullman, Michael Moore filed Critical Sangamo Biosciences Inc
Priority to AU2001226921A priority Critical patent/AU2001226921A1/en
Publication of WO2001053479A2 publication Critical patent/WO2001053479A2/fr
Publication of WO2001053479A3 publication Critical patent/WO2001053479A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4703—Inhibitors; Suppressors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé de sélection d'un ou plusieurs composants d'un système activateur composé (I) d'une première molécule et (II) d'une deuxième molécule, la première molécule se liant à la deuxième de manière modulable par un ligand, et (III) d'un ligand. Ledit procédé consiste (a) à déterminer le degré de liaison entre une première molécule candidate et une deuxième molécule candidate en présence d'un ligand candidat, (b) à déterminer le degré de liaison entre la première molécule candidate et la deuxième molécule candidate en l'absence du ligand candidat, (c) à identifier un couple première molécule/deuxième molécule dans laquelle la liaison de la première molécule à la deuxième diffère selon la présence ou l'absence d'un ligand, et (d) à isoler et/ou identifier éventuellement la première molécule, la deuxième molécule, ou le ligand.
PCT/GB2001/000187 2000-01-24 2001-01-18 Sequences activatrices ii WO2001053479A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001226921A AU2001226921A1 (en) 2000-01-24 2001-01-18 Molecular switches ii

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
GB0001578.4 2000-01-24
GB0001578A GB0001578D0 (en) 2000-01-24 2000-01-24 Gene switches
GB0001582A GB0001582D0 (en) 2000-01-24 2000-01-24 Gene switches
GB0001582.6 2000-01-24
GBPCT/GB00/02071 2000-05-30
GBPCT/GB00/02080 2000-05-30
PCT/GB2000/002080 WO2001000815A1 (fr) 1999-05-28 2000-05-30 Commutateurs moleculaires
PCT/GB2000/002071 WO2000073434A1 (fr) 1999-05-28 2000-05-30 Commutateurs genetiques
GBGB0029901.6A GB0029901D0 (en) 2000-01-24 2000-12-07 Molecular switches II
GB0029901.6 2000-12-07

Publications (2)

Publication Number Publication Date
WO2001053479A2 WO2001053479A2 (fr) 2001-07-26
WO2001053479A3 true WO2001053479A3 (fr) 2002-01-31

Family

ID=27447759

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/000187 WO2001053479A2 (fr) 2000-01-24 2001-01-18 Sequences activatrices ii

Country Status (1)

Country Link
WO (1) WO2001053479A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE520028T1 (de) * 2003-06-20 2011-08-15 Discoverx Corp Test zum nachweis von proteinbindung
US8329889B2 (en) 2008-02-15 2012-12-11 Trustees Of Boston University In vivo gene sensors

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016429A1 (fr) * 1990-04-19 1991-10-31 The General Hospital Corporation Techniques de triage de partenaires de proteines et leurs utilisations
WO1992000388A1 (fr) * 1990-07-02 1992-01-09 The Regents Of The University Of California Detection d'analytes par transfert fluorescent d'energie
WO1993023431A1 (fr) * 1992-05-14 1993-11-25 Baylor College Of Medicine Recepteurs d'hormones steroides mutees, procede d'utilisation et commutateur moleculaire pour therapie genique
WO1995029195A1 (fr) * 1994-04-22 1995-11-02 California Institute Of Technology Detecteur de proteines morcelees a base d'ubiquitine
WO1996040892A1 (fr) * 1995-06-07 1996-12-19 Basf Ag Modulateurs de transcription regules par la tetracycline et presentant des specificites de fixation de l'adn modifiees
WO1998034120A1 (fr) * 1997-01-31 1998-08-06 Universite De Montreal Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires
WO1998044350A1 (fr) * 1997-04-02 1998-10-08 The Board Of Trustees Of The Leland Stanford Junior University Detection d'interactions moleculaires par complementation de sous-unites de marquage
WO1999000488A1 (fr) * 1997-06-30 1999-01-07 The Regents Of The University Of California Procede de criblage des facteurs de transcription nucleaire en fonction de leur aptitude a moduler une reponse des oestrogenes
WO1999028746A1 (fr) * 1997-12-04 1999-06-10 Institut Pasteur Systeme bacterien comportant des hybrides multiples et ses mises en application
WO2000009704A1 (fr) * 1998-08-13 2000-02-24 Syngenta Limited Commutateur de gene
WO2000052179A2 (fr) * 1999-03-03 2000-09-08 Genelabs Technologies, Inc. Syteme de sequence activatrice a mediation assuree par compose de liaison a l'adn
WO2000073434A1 (fr) * 1999-05-28 2000-12-07 Sangamo Biosciences, Inc. Commutateurs genetiques
WO2001000815A1 (fr) * 1999-05-28 2001-01-04 Sangamo Biosciences, Inc. Commutateurs moleculaires

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016429A1 (fr) * 1990-04-19 1991-10-31 The General Hospital Corporation Techniques de triage de partenaires de proteines et leurs utilisations
WO1992000388A1 (fr) * 1990-07-02 1992-01-09 The Regents Of The University Of California Detection d'analytes par transfert fluorescent d'energie
WO1993023431A1 (fr) * 1992-05-14 1993-11-25 Baylor College Of Medicine Recepteurs d'hormones steroides mutees, procede d'utilisation et commutateur moleculaire pour therapie genique
WO1995029195A1 (fr) * 1994-04-22 1995-11-02 California Institute Of Technology Detecteur de proteines morcelees a base d'ubiquitine
WO1996040892A1 (fr) * 1995-06-07 1996-12-19 Basf Ag Modulateurs de transcription regules par la tetracycline et presentant des specificites de fixation de l'adn modifiees
WO1998034120A1 (fr) * 1997-01-31 1998-08-06 Universite De Montreal Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires
WO1998044350A1 (fr) * 1997-04-02 1998-10-08 The Board Of Trustees Of The Leland Stanford Junior University Detection d'interactions moleculaires par complementation de sous-unites de marquage
WO1999000488A1 (fr) * 1997-06-30 1999-01-07 The Regents Of The University Of California Procede de criblage des facteurs de transcription nucleaire en fonction de leur aptitude a moduler une reponse des oestrogenes
WO1999028746A1 (fr) * 1997-12-04 1999-06-10 Institut Pasteur Systeme bacterien comportant des hybrides multiples et ses mises en application
WO2000009704A1 (fr) * 1998-08-13 2000-02-24 Syngenta Limited Commutateur de gene
WO2000052179A2 (fr) * 1999-03-03 2000-09-08 Genelabs Technologies, Inc. Syteme de sequence activatrice a mediation assuree par compose de liaison a l'adn
WO2000073434A1 (fr) * 1999-05-28 2000-12-07 Sangamo Biosciences, Inc. Commutateurs genetiques
WO2001000815A1 (fr) * 1999-05-28 2001-01-04 Sangamo Biosciences, Inc. Commutateurs moleculaires

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BULYK MARTHA L ET AL: "Exploring the DNA-binding specificities of zinc fingers with DNA microarrays.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 98, no. 13, 19 June 2001 (2001-06-19), June 19, 2001, pages 7158 - 7163, XP002174591, ISSN: 0027-8424 *
DICKINSON L A ET AL: "INHIBITION OF ETS-1 DNA BINDING AND TERNARY COMPLEX FORMATION BETWEEN ETS-1, NF-KB, AND DNA BY A DESIGNED DNA-BINDING LIGAND", JOURNAL OF BIOLOGICAL CHEMISTRY,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD,US, vol. 274, no. 18, 1999, pages 12765 - 12773, XP000901235, ISSN: 0021-9258 *
M.L. BULYK ET AL.: "Quantifying DNA-protein interactions by double-stranded DNA arrays", NATURE BIOTECHNOLOGY, vol. 17, no. 6, June 1999 (1999-06-01), NATURE PUBL. CO.,NEW YORK, US, pages 573 - 577, XP002174590 *

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