+

WO2000017365A3 - Binary viral expression system in plants - Google Patents

Binary viral expression system in plants Download PDF

Info

Publication number
WO2000017365A3
WO2000017365A3 PCT/US1999/021989 US9921989W WO0017365A3 WO 2000017365 A3 WO2000017365 A3 WO 2000017365A3 US 9921989 W US9921989 W US 9921989W WO 0017365 A3 WO0017365 A3 WO 0017365A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
expression system
viral expression
binary
binary viral
Prior art date
Application number
PCT/US1999/021989
Other languages
French (fr)
Other versions
WO2000017365A2 (en
WO2000017365A8 (en
Inventor
Narendra S Yadav
Original Assignee
Du Pont
Narendra S Yadav
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Narendra S Yadav filed Critical Du Pont
Priority to AU14410/00A priority Critical patent/AU1441000A/en
Priority to EP99969445A priority patent/EP1115870A2/en
Priority to CA002339595A priority patent/CA2339595A1/en
Priority to NZ510594A priority patent/NZ510594A/en
Priority to HU0104697A priority patent/HUP0104697A3/en
Publication of WO2000017365A2 publication Critical patent/WO2000017365A2/en
Publication of WO2000017365A3 publication Critical patent/WO2000017365A3/en
Publication of WO2000017365A8 publication Critical patent/WO2000017365A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/12011—Geminiviridae
    • C12N2750/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to plant transgene expression systems comprising chromosomally-integrated components that are individually heritable. The systems are useful for the regulated expression of foreign genes through gene amplification in plant tissue.
PCT/US1999/021989 1998-09-23 1999-09-22 Binary viral expression system in plants WO2000017365A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU14410/00A AU1441000A (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
EP99969445A EP1115870A2 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
CA002339595A CA2339595A1 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
NZ510594A NZ510594A (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
HU0104697A HUP0104697A3 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US10155898P 1998-09-23 1998-09-23
US60/101,558 1998-09-23
US13008699P 1999-04-20 1999-04-20
US69/130,086 1999-04-20
US15025599P 1999-08-23 1999-08-23
US60/150,255 1999-08-23

Publications (3)

Publication Number Publication Date
WO2000017365A2 WO2000017365A2 (en) 2000-03-30
WO2000017365A3 true WO2000017365A3 (en) 2000-08-24
WO2000017365A8 WO2000017365A8 (en) 2000-10-12

Family

ID=27379204

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/021989 WO2000017365A2 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants

Country Status (5)

Country Link
EP (1) EP1115870A2 (en)
CA (1) CA2339595A1 (en)
HU (1) HUP0104697A3 (en)
NZ (1) NZ510594A (en)
WO (1) WO2000017365A2 (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6632980B1 (en) 1997-10-24 2003-10-14 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
DE60131075T2 (en) 2000-03-28 2008-07-31 Queensland University Of Technology, Brisbane CONSTRUCTED BY RELEASE IN CLOSED, CIRCULAR FORM FROM A LARGER NUCLEOTIDE SEQUENCE WHICH ALLOWS THE SITE-SPECIFIC AND / OR DEVELOPMENT-SPECIFIC, REGULATED EXPRESSION OF SELECTED GENETIC SEQUENCES
AU2001243939B2 (en) * 2000-03-28 2006-06-15 Queensland University Of Technology A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences
US7468475B2 (en) 2000-06-16 2008-12-23 Schmuelling Thomas Method for modifying plant morphology, biochemistry and physiology
DE10049587A1 (en) 2000-10-06 2002-05-02 Icon Genetics Ag Vector system for plants
US7560622B2 (en) 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
DE10061150A1 (en) 2000-12-08 2002-06-13 Icon Genetics Ag Methods and vectors for the production of transgenic plants
DE10102389A1 (en) 2001-01-19 2002-08-01 Icon Genetics Ag Methods and vectors for plastid transformation of higher plants
DE10109354A1 (en) * 2001-02-27 2002-09-05 Icon Genetics Ag Recombinant viral switch systems
DE10114209A1 (en) 2001-03-23 2002-12-05 Icon Genetics Ag Site-directed transformation using amplification vectors
DE10115507A1 (en) 2001-03-29 2002-10-10 Icon Genetics Ag Method for coding information in nucleic acids of a genetically modified organism
DE10121283B4 (en) * 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Methods and vectors for amplification or expression of desired nucleic acid sequences in plants
DE10132780A1 (en) 2001-07-06 2003-01-16 Icon Genetics Ag Plastid gene expression via autonomously replicating vectors
DE10143237A1 (en) 2001-09-04 2003-03-20 Icon Genetics Ag Manufacture of artificial internal ribosomal entry point elements (Ires elements)
DE10143238A1 (en) 2001-09-04 2003-03-20 Icon Genetics Ag Identification of eukaryotic internal ribosome entry sites (IRES) elements
US7238854B2 (en) 2002-04-11 2007-07-03 E. I. Du Pont De Nemours And Company Method of controlling site-specific recombination
WO2004003180A1 (en) 2002-07-01 2004-01-08 E.I. Du Pont De Nemours And Company Method of controlling gene silencing using site-specific recombination
PL2121935T3 (en) 2006-09-25 2011-12-30 Schmuelling Thomas Transcriptional repressors of cytokinin signaling and their use
ES2385451T3 (en) 2009-07-10 2012-07-25 Freie Universität Berlin Rock2 and Rock3, two new variants of function gain of cytokinin receptors AHK2 and AHK3
EP2418283A1 (en) 2010-08-07 2012-02-15 Nomad Bioscience GmbH Process of transfecting plants
EP2584042A1 (en) 2011-10-17 2013-04-24 Nomad Bioscience GmbH Production, storage and use of cell wall-degrading enzymes
EP2647715A1 (en) 2012-04-03 2013-10-09 Nomad Bioscience GmbH Agrobacterium for transient transfection of whole plants
EP3097783B1 (en) 2015-05-26 2019-11-13 Nomad Bioscience GmbH Colicin m for the control of ehec
EP3372091A1 (en) 2017-03-07 2018-09-12 Nomad Bioscience GmbH Method of reducing contamination of an object with clostridium
EP3378485A1 (en) 2017-03-24 2018-09-26 Nomad Bioscience GmbH Bacteriocins for control of salmonella enterica
JP2021514391A (en) 2018-02-15 2021-06-10 アイコン ジェネティクス ゲーエムベーハー Immunogenic compositions and vaccines to generate an immune response against norovirus
JP7536031B2 (en) 2019-03-12 2024-08-19 アイコン ジェネティクス ゲーエムベーハー Norovirus-like particles with improved stability
BR112021024197A2 (en) 2019-06-06 2022-04-26 Nomad Bioscience Gmbh Klebicins for the control of klebsiella
BR112022027078A2 (en) 2020-07-16 2023-01-31 Nomad Bioscience Gmbh PRODUCTS FOR ORAL CONSUMPTION WITH REDUCED SUGAR CONTENT
EP4248987A1 (en) 2022-03-21 2023-09-27 Nomad Bioscience GmbH Chimeric bacteriocins and method for the control of pseudomonas

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0221044A1 (en) * 1985-10-25 1987-05-06 Monsanto Company Novel plant vectors
EP0425004A2 (en) * 1989-10-03 1991-05-02 Aveve N.V. Genetic manipulations with recombinant DNA comprising sequences derived from RNA virus
WO1994019477A1 (en) * 1993-02-26 1994-09-01 Calgene Inc. Geminivirus-based gene expression system
WO1995025801A2 (en) * 1994-03-23 1995-09-28 University Of Leicester Viral replicon
WO1998028431A1 (en) * 1996-12-24 1998-07-02 Plant Bioscience Limited Transcriptional regulation in plants
WO1998036083A1 (en) * 1997-02-14 1998-08-20 Plant Bioscience Limited Methods and means for gene silencing in transgenic plants
WO1999025855A1 (en) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from t-dna using site-specific recombination systems

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0221044A1 (en) * 1985-10-25 1987-05-06 Monsanto Company Novel plant vectors
EP0425004A2 (en) * 1989-10-03 1991-05-02 Aveve N.V. Genetic manipulations with recombinant DNA comprising sequences derived from RNA virus
WO1994019477A1 (en) * 1993-02-26 1994-09-01 Calgene Inc. Geminivirus-based gene expression system
WO1995025801A2 (en) * 1994-03-23 1995-09-28 University Of Leicester Viral replicon
WO1998028431A1 (en) * 1996-12-24 1998-07-02 Plant Bioscience Limited Transcriptional regulation in plants
WO1998036083A1 (en) * 1997-02-14 1998-08-20 Plant Bioscience Limited Methods and means for gene silencing in transgenic plants
WO1999025855A1 (en) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from t-dna using site-specific recombination systems

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANGELL S M ET AL: "Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA.", EMBO JOURNAL, (1997 JUN) 16 (12) 3675-84., XP002135935 *
HAYES R J ET AL: "Replication of tomato golden mosaic virus DNA B in transgenic plants expressing open reading frames (ORFs) of DNA A: requirement of ORF AL2 for production of single-stranded DNA", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 17, no. 24, 25 December 1989 (1989-12-25), pages 10213 - 10222, XP002093974, ISSN: 0305-1048 *
HONG Y ET AL: "Transactivation of dianthin transgene expression by African cassava mosaic virus AC2", VIROLOGY,US,ACADEMIC PRESS,ORLANDO, vol. 228, no. 2, 17 February 1997 (1997-02-17), pages 383 - 387, XP002093972, ISSN: 0042-6822 *
PRUSS G. ET AL.: "Plant viral synergism: The potyviral genome encodes a broad-range pathogenicity enhancer that transactivates replication of heterologous viruses", PLANT CELL, vol. 9, 1997, pages 859 - 868, XP002078748 *

Also Published As

Publication number Publication date
WO2000017365A2 (en) 2000-03-30
NZ510594A (en) 2002-09-27
HUP0104697A3 (en) 2003-12-29
EP1115870A2 (en) 2001-07-18
HUP0104697A2 (en) 2002-03-28
WO2000017365A8 (en) 2000-10-12
CA2339595A1 (en) 2000-03-30

Similar Documents

Publication Publication Date Title
WO2000017365A3 (en) Binary viral expression system in plants
ES2180641T3 (en) CYTOPLASMIC INHIBITION OF GENETIC EXPRESSION.
WO2000007430A3 (en) Plant fatty acid desaturase promoters
HK1034999A1 (en) Dsrna-mediated regulation of gene expression in plants.
AU6871398A (en) Method for enhancing expression of a foreign or endogenous gene product in pla nts
WO1995031564A3 (en) Method of introducing pathogen resistance in plants
WO2000053724A3 (en) Compositions and methods for the modification of gene transcription
HUP9902317A2 (en) Nucleic acid molecules encoding debranching enzymes from potatoes
HUP0002578A2 (en) Male tissue-preferred regulatory region and method of using same
WO2000031283A3 (en) Regulatory sequences useful for gene expression in plant embryo tissue
DE60042178D1 (en) Genes encoding flavone synthases
WO2000026389A3 (en) Dna comprising rice anther-specific gene and transgenic plant transformed therewith
WO2000063397A3 (en) Methods for delivering inhibitory rna to plants and applications thereof
ATE280224T1 (en) PROMOTER ELEMENTS WHICH PROVIDE ROOT PREFERRED GENE EXPRESSION
AU7532998A (en) Recombinant construct for enhancement of gene expression in plants
AU5559301A (en) Nucleic acids that control plant development
UA70915C2 (en) A method for inducing the viral resistance in plants
WO2001012798A3 (en) Male sterile plants
AU5539500A (en) Promoter enabling transgene expression in the whole plant except in the seed
WO1999055891A8 (en) Plant gene that regulates dna methylation
WO2001012799A3 (en) Regulatory sequences for pollen specific or pollen abundant gene expression in plants
MXPA01009366A (en) Plants having modified reproductive capacity.
TR200002555T2 (en) Methods for plant transformation and regeneration.
AR008231A1 (en) AN ISOLATED NUCLEIC ACID SEQUENCE, A VECTOR UNDERSTANDING IT, A TRANSGENIC PLANT CELL, A SEED OF SUCH A PLANT AND A METHOD TO REDUCE THE TRANSCRIPTION OF A DNA SEQUENCE IN A TRANSGENIC PLANT
WO2002014521A3 (en) Plants having modified growth properties

Legal Events

Date Code Title Description
ENP Entry into the national phase

Ref country code: AU

Ref document number: 2000 14410

Kind code of ref document: A

Format of ref document f/p: F

AK Designated states

Kind code of ref document: A2

Designated state(s): AU BR CA HU IL JP KR MX NZ PL US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AU BR CA HU IL JP KR MX NZ PL US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

AK Designated states

Kind code of ref document: C1

Designated state(s): AU BR CA HU IL JP KR MX NZ PL US

AL Designated countries for regional patents

Kind code of ref document: C1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: PAT. BUL. 13/2000 UNDER (30) REPLACE "NOT FURNISHED" BY "60/150255"

ENP Entry into the national phase

Ref document number: 2339595

Country of ref document: CA

Ref country code: CA

Ref document number: 2339595

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 1999969445

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 14410/00

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: PA/a/2001/002374

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 09786676

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 510594

Country of ref document: NZ

WWP Wipo information: published in national office

Ref document number: 1999969445

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999969445

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载